##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1545274_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 3170271 Sequences flagged as poor quality 0 Sequence length 51 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.242877343924228 31.0 31.0 33.0 30.0 34.0 2 31.55490808199047 31.0 31.0 34.0 30.0 34.0 3 31.659105798841804 31.0 31.0 34.0 30.0 34.0 4 30.301599453169775 35.0 33.0 37.0 0.0 37.0 5 32.84743102403549 35.0 33.0 37.0 19.0 37.0 6 34.4611097915604 35.0 33.0 37.0 30.0 37.0 7 35.47520606282554 36.0 35.0 37.0 33.0 37.0 8 35.65075509317658 37.0 35.0 37.0 35.0 37.0 9 37.64503097684709 39.0 37.0 39.0 35.0 39.0 10 37.10148627672524 39.0 37.0 39.0 33.0 39.0 11 36.666490341046554 39.0 35.0 39.0 32.0 39.0 12 35.575796832510534 37.0 35.0 39.0 31.0 39.0 13 35.12870066943804 37.0 35.0 39.0 30.0 39.0 14 35.9590893018294 38.0 35.0 40.0 30.0 41.0 15 36.28339438489643 38.0 35.0 40.0 31.0 41.0 16 36.46244027718766 38.0 35.0 40.0 32.0 41.0 17 36.398322414708396 37.0 35.0 40.0 31.0 41.0 18 36.37490517372174 37.0 35.0 40.0 31.0 41.0 19 36.35714328522704 37.0 35.0 40.0 31.0 41.0 20 36.25609419510193 37.0 35.0 40.0 31.0 41.0 21 36.08265949504001 37.0 34.0 40.0 31.0 41.0 22 35.94385685009263 37.0 34.0 40.0 30.0 41.0 23 35.90521788200441 36.0 34.0 40.0 30.0 41.0 24 35.83766718996578 36.0 34.0 40.0 30.0 41.0 25 35.720991044614166 36.0 34.0 40.0 30.0 41.0 26 35.55431601904064 36.0 34.0 40.0 30.0 41.0 27 35.55614109960946 36.0 34.0 40.0 30.0 41.0 28 35.59462266790442 36.0 34.0 40.0 30.0 41.0 29 35.62155443493632 36.0 34.0 40.0 30.0 41.0 30 35.57985358349491 36.0 34.0 40.0 30.0 41.0 31 35.48115602735539 36.0 34.0 40.0 30.0 41.0 32 35.36769254111084 36.0 34.0 40.0 29.0 41.0 33 35.221278559466995 36.0 34.0 40.0 29.0 41.0 34 35.13098407044698 36.0 34.0 40.0 29.0 41.0 35 35.01727581017522 35.0 34.0 40.0 28.0 41.0 36 34.84415527883894 35.0 33.0 40.0 27.0 41.0 37 34.79825163211599 35.0 33.0 40.0 27.0 41.0 38 34.78982206883891 35.0 33.0 40.0 27.0 41.0 39 34.726956780666384 35.0 33.0 40.0 27.0 41.0 40 34.566595095498144 35.0 33.0 40.0 26.0 41.0 41 34.54024245876772 35.0 33.0 40.0 26.0 41.0 42 34.49812776257929 35.0 33.0 40.0 26.0 41.0 43 34.43199082980603 35.0 33.0 40.0 27.0 41.0 44 34.36752567840415 35.0 33.0 39.0 27.0 41.0 45 34.280867156151636 35.0 33.0 39.0 26.0 41.0 46 34.20415226332386 35.0 33.0 39.0 26.0 41.0 47 34.139688373643764 35.0 33.0 39.0 26.0 41.0 48 34.03873422808334 35.0 33.0 39.0 26.0 41.0 49 33.997886931432674 35.0 33.0 39.0 26.0 41.0 50 33.85879062073873 35.0 33.0 39.0 25.0 40.0 51 33.77415085334976 35.0 33.0 39.0 25.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 1.0 11 1.0 12 5.0 13 5.0 14 28.0 15 65.0 16 229.0 17 668.0 18 1464.0 19 2952.0 20 5089.0 21 8075.0 22 12335.0 23 18335.0 24 25658.0 25 34653.0 26 44772.0 27 56100.0 28 67595.0 29 81965.0 30 100709.0 31 127188.0 32 162052.0 33 235447.0 34 353567.0 35 474195.0 36 235058.0 37 291931.0 38 375251.0 39 454570.0 40 307.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.5486054662204 25.21279726559654 30.280629006163828 11.957968262019241 2 28.18065080240774 26.725980208001143 31.633541738229948 13.459827251361162 3 29.082308736382473 26.537037369991403 30.75702361091528 13.623630282710847 4 23.610789109196027 38.285654444052255 25.746978728316915 12.356577718434798 5 23.7892281133064 32.53586207614428 28.99862503867966 14.67628477186966 6 22.463631657987598 41.57565709682232 25.09116728506806 10.869543960122021 7 89.48544146541416 4.326033957349387 4.306256468295612 1.8822681089408444 8 91.14328081100953 2.998671091524983 3.7434970070382 2.1145510904272853 9 87.2586917648365 4.66720352928819 5.203309117737884 2.870795588137418 10 58.55761226721627 22.83609193031132 9.08086406493325 9.525431737539156 11 51.08881858995651 19.909086636442122 15.786473774639456 13.215620998961919 12 47.63800949508733 20.27810871688887 18.348526040833733 13.735355747190066 13 23.058060336166843 40.52940584574631 18.687992288356423 17.724541529730423 14 15.196051063142551 43.01364142055994 23.652520557390837 18.13778695890667 15 15.250242014010789 23.551803615526875 46.00572001573367 15.192234354728665 16 16.98072499164898 18.232384550090515 44.86666912702415 19.920221331236352 17 17.72372141056711 19.528614430753713 25.68345103620479 37.064213122474385 18 22.133344436485082 25.32316637915181 30.30160513091783 22.241884053445276 19 33.80985411026376 25.694648817088506 20.317285178459507 20.178211894188223 20 36.94447572463048 23.737119003391193 20.838880966327483 18.47952430565084 21 25.22185011943774 29.34332743162966 23.70093913107113 21.73388331786147 22 23.79250858995966 26.07505793668743 21.3364409541014 28.795992519251506 23 23.70961346837542 31.76185884424391 21.369624237170893 23.158903450209777 24 23.6162145128918 21.696599438975404 35.375114619538834 19.31207142859396 25 20.636879307794192 24.631080434448663 31.555441159446623 23.176599098310525 26 19.43559399180701 36.798526056605255 22.47287377009726 21.29300618149048 27 20.471530667252104 34.792514583138164 25.465173166584183 19.27078158302555 28 17.79504023473072 29.560501294684272 34.877207658272745 17.76725081231226 29 18.097758835127976 26.223026359576203 33.63403317886704 22.045181626428782 30 21.461067523880452 31.01469874341973 26.188928328209165 21.335305404490658 31 31.528534942280963 27.235589638866838 20.30211297393819 20.933762444914013 32 33.79184303171559 27.890297075549693 20.944802510574018 17.373057382160706 33 30.590255533359766 27.924615908229928 20.734978176944495 20.75015038146581 34 20.731855415514953 28.053153815557092 25.34988333804902 25.865107430878936 35 22.660838773719973 24.399049797320167 28.688178392320403 24.251933036639453 36 31.313348291045152 25.826530287158416 22.201572042263894 20.658549379532538 37 22.331466300515004 33.80376630262838 25.124697541629722 18.74006985522689 38 22.941887302378884 32.523371030426105 20.91433192935241 23.6204097378426 39 23.433864171233314 31.38327922124008 23.090234241804563 22.092622365722047 40 27.36359131443337 24.286693471946087 24.559572352016595 23.790142861603943 41 17.843774238858444 24.323472662116266 26.908835238375524 30.92391786064977 42 23.035854032667867 26.261729675475692 21.43671629333896 29.265699998517476 43 23.24618305501328 26.439380103467496 23.43651378699171 26.877923054527514 44 21.034132413285807 30.15616015160849 26.193785957099568 22.61592147800614 45 19.63601849810316 35.450060893847876 20.19095528426434 24.722965323784624 46 21.343411966989574 34.00772362993574 22.188229334337663 22.460635068737027 47 23.021438861220382 26.21741169761197 24.84778115183213 25.91336828933552 48 24.37551868594199 21.82315013448377 30.472316089066204 23.329015090508033 49 21.38015961411501 23.690466840216498 30.235743253494736 24.69363029217376 50 18.803912977786442 32.90866932196017 24.56294745780408 23.724470242449307 51 17.887524441916796 33.93227266691081 22.583684486278933 25.59651840489346 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 576.0 1 1243.0 2 1910.0 3 4798.0 4 7686.0 5 5882.5 6 4079.0 7 4406.5 8 4734.0 9 5086.5 10 5439.0 11 5802.5 12 6166.0 13 6127.5 14 6089.0 15 5793.0 16 5497.0 17 5406.5 18 5316.0 19 5435.5 20 5555.0 21 7175.0 22 8795.0 23 9613.5 24 10432.0 25 11068.0 26 14845.5 27 17987.0 28 23393.5 29 28800.0 30 37115.5 31 45431.0 32 50841.5 33 56252.0 34 58997.0 35 61742.0 36 66329.5 37 70917.0 38 92309.0 39 113701.0 40 168342.5 41 222984.0 42 268767.0 43 314550.0 44 305702.0 45 296854.0 46 284427.0 47 272000.0 48 258561.5 49 245123.0 50 223531.0 51 201939.0 52 184800.5 53 167662.0 54 160156.5 55 152651.0 56 152619.0 57 152587.0 58 158637.0 59 164687.0 60 161851.0 61 159015.0 62 140625.5 63 122236.0 64 97027.0 65 71818.0 66 58710.5 67 45603.0 68 40560.0 69 35517.0 70 31928.5 71 28340.0 72 24059.5 73 19779.0 74 13964.5 75 6769.0 76 5388.0 77 4115.5 78 2843.0 79 1912.0 80 981.0 81 688.0 82 395.0 83 291.0 84 187.0 85 154.0 86 121.0 87 80.5 88 40.0 89 24.5 90 9.0 91 6.5 92 4.0 93 2.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 3170271.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 15.212916651663102 #Duplication Level Percentage of deduplicated Percentage of total 1 77.51690642128041 11.79258236481707 2 9.653004796718497 2.937007148211652 3 3.3923399965362013 1.5482215686422496 4 1.6933018608020698 1.030402402979517 5 0.9828922300097038 0.747632878635245 6 0.687407640370313 0.6274485083201985 7 0.42064515291459803 0.4479467755851102 8 0.32483432634304177 0.3953342025804661 9 0.22934099107807132 0.3140050843872459 >10 2.107167221678657 7.573195808196337 >50 0.9860215201793446 11.027131375259527 >100 1.9766990885202163 57.81958464082775 >500 0.024230358706668488 2.372498914882634 >1k 0.005208394862181077 1.3670083266750566 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG 3889 0.12267090100499295 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3435 0.10835035869173329 No Hit GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT 3283 0.10355581589081818 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 6.308608948572535E-5 0.0 0.0 0.07371609556407008 0.0 2 6.308608948572535E-5 0.0 0.0 0.24934776869232947 0.0 3 6.308608948572535E-5 0.0 0.0 0.3576665843393199 0.0 4 6.308608948572535E-5 0.0 0.0 0.5919998637340467 0.0 5 6.308608948572535E-5 0.0 0.0 0.985877863438173 0.0 6 6.308608948572535E-5 0.0 0.0 1.379787406187042 0.0 7 9.462913422858803E-5 0.0 0.0 1.5691087607337038 0.0 8 9.462913422858803E-5 0.0 0.0 2.0399202465656723 0.0 9 9.462913422858803E-5 0.0 0.0 2.172937266246324 0.0 10 9.462913422858803E-5 0.0 0.0 2.392129884164477 0.0 11 9.462913422858803E-5 0.0 0.0 2.736958449293452 0.0 12 9.462913422858803E-5 0.0 0.0 3.0064306805317274 0.0 13 1.261721789714507E-4 0.0 0.0 3.1224459990959765 0.0 14 1.261721789714507E-4 0.0 0.0 3.166637804780727 0.0 15 1.261721789714507E-4 0.0 0.0 3.242656542611026 0.0 16 1.261721789714507E-4 0.0 0.0 3.4099293088824267 0.0 17 1.261721789714507E-4 0.0 0.0 3.5997868951897174 0.0 18 1.261721789714507E-4 0.0 0.0 3.8227331354322707 0.0 19 1.261721789714507E-4 0.0 0.0 3.957768909976466 0.0 20 1.261721789714507E-4 0.0 0.0 4.090628214433403 0.0 21 1.261721789714507E-4 0.0 0.0 4.274650337463264 0.0 22 1.261721789714507E-4 0.0 0.0 4.467031367349984 0.0 23 1.261721789714507E-4 0.0 0.0 4.679158343245735 0.0 24 1.261721789714507E-4 0.0 0.0 4.839302381405249 0.0 25 1.261721789714507E-4 0.0 0.0 4.968881209208929 0.0 26 1.261721789714507E-4 0.0 0.0 5.091741368482379 0.0 27 1.261721789714507E-4 0.0 0.0 5.2138760377267435 0.0 28 1.261721789714507E-4 0.0 0.0 5.3422246867854515 0.0 29 1.5771522371431338E-4 0.0 0.0 5.488584414392334 0.0 30 1.5771522371431338E-4 0.0 0.0 5.658096736840478 0.0 31 1.8925826845717607E-4 0.0 0.0 5.836031052234967 0.0 32 1.8925826845717607E-4 0.0 0.0 5.997752242631623 0.0 33 2.2080131320003873E-4 0.0 0.0 6.1485910825919925 0.0 34 2.523443579429014E-4 0.0 0.0 6.306085504993106 0.0 35 2.523443579429014E-4 0.0 0.0 6.509380428360856 0.0 36 2.523443579429014E-4 0.0 0.0 6.694064955330317 0.0 37 2.523443579429014E-4 0.0 0.0 6.885531236919493 0.0 38 2.523443579429014E-4 0.0 0.0 7.056336824202095 0.0 39 2.523443579429014E-4 0.0 0.0 7.239759629381841 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTATAA 20 7.0351665E-4 45.0 18 GTCGACG 25 3.8923645E-5 45.0 11 CGCATCG 20 7.0351665E-4 45.0 21 ATTACGC 25 3.8923645E-5 45.0 31 ATAGCCG 20 7.0351665E-4 45.0 10 TCGATCG 20 7.0351665E-4 45.0 1 ATCCGAT 45 3.8562575E-10 45.0 45 AATGCGT 20 7.0351665E-4 45.0 37 GTCGTAC 25 3.8923645E-5 45.0 32 GAACGTA 45 3.8562575E-10 45.0 11 TTGCGAA 20 7.0351665E-4 45.0 28 AACGACC 25 3.8923645E-5 45.0 20 ACGTCGA 20 7.0351665E-4 45.0 43 ACCCGTA 20 7.0351665E-4 45.0 33 GTACGTA 40 6.8212103E-9 45.0 35 AGTCGAC 25 3.8923645E-5 45.0 10 GTACGAC 25 3.8923645E-5 45.0 19 CTTACGC 20 7.0351665E-4 45.0 11 TCTACGT 30 2.1667147E-6 44.999996 20 GTTCGGA 30 2.1667147E-6 44.999996 42 >>END_MODULE