FastQCFastQC Report
Thu 26 May 2016
SRR1545273_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545273_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2075682
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24490.11798531759681877No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTACG253.8918955E-545.0000041
TCGATCT253.8918955E-545.00000415
ATCGTAC253.8918955E-545.00000416
CACCGTA253.8918955E-545.00000434
TACGTCA253.8918955E-545.00000432
CGCTTAT253.8918955E-545.00000433
ATCGTTC207.0346025E-445.016
CATTACG207.0346025E-445.012
CCGTACT406.8193913E-945.033
TATAGCG1250.044.9999961
ATACGAC302.166351E-644.99999622
ACGTTAG650.041.538461
CGGGTAT1850.041.3513536
TATCGTG556.184564E-1140.9090921
GTACTAT1050.040.71428739
CCGCAAT501.0822987E-940.50000433
TAATACG451.929584E-840.01
CTTACCG451.929584E-840.01
CGTATTT3950.039.8734171
ATAGGGA17250.039.7826084