##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545273_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2075682
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.268251109755735	31.0	31.0	33.0	30.0	34.0
2	31.600036036348534	31.0	31.0	34.0	30.0	34.0
3	31.710303408710967	31.0	31.0	34.0	30.0	34.0
4	30.342374699014588	35.0	33.0	37.0	0.0	37.0
5	32.90571725341358	35.0	33.0	37.0	19.0	37.0
6	34.5437831999314	35.0	33.0	37.0	30.0	37.0
7	35.465860377456664	36.0	35.0	37.0	33.0	37.0
8	35.63089240066638	37.0	35.0	37.0	35.0	37.0
9	37.66487014870293	39.0	37.0	39.0	35.0	39.0
10	37.15568425221204	39.0	37.0	39.0	33.0	39.0
11	36.728342299061225	39.0	35.0	39.0	32.0	39.0
12	35.646462223018744	37.0	35.0	39.0	31.0	39.0
13	35.20498371137776	37.0	35.0	39.0	30.0	39.0
14	35.972741489303274	38.0	35.0	40.0	30.0	41.0
15	36.2756626496737	38.0	35.0	40.0	31.0	41.0
16	36.453598865336794	38.0	35.0	40.0	31.0	41.0
17	36.421426307112554	38.0	35.0	40.0	31.0	41.0
18	36.40783752039089	37.0	35.0	40.0	31.0	41.0
19	36.381192783865735	37.0	35.0	40.0	31.0	41.0
20	36.24564600935982	37.0	35.0	40.0	31.0	41.0
21	36.08224188483592	37.0	34.0	40.0	31.0	41.0
22	35.98755686082936	37.0	34.0	40.0	30.0	41.0
23	35.94589970910766	37.0	34.0	40.0	30.0	41.0
24	35.85033979193344	36.0	34.0	40.0	30.0	41.0
25	35.71219917116398	36.0	34.0	40.0	30.0	41.0
26	35.555952694102466	36.0	34.0	40.0	30.0	41.0
27	35.56359500154648	36.0	34.0	40.0	30.0	41.0
28	35.567949714840715	36.0	34.0	40.0	30.0	41.0
29	35.593872279087066	36.0	34.0	40.0	30.0	41.0
30	35.55240349918726	36.0	34.0	40.0	30.0	41.0
31	35.406027031115556	36.0	34.0	40.0	29.0	41.0
32	35.29129413850484	36.0	34.0	40.0	29.0	41.0
33	35.16284864444554	36.0	34.0	40.0	29.0	41.0
34	35.06081230169168	36.0	34.0	40.0	28.0	41.0
35	34.9044622442166	35.0	34.0	40.0	27.0	41.0
36	34.71567610067438	35.0	33.0	40.0	26.0	41.0
37	34.656466163892155	35.0	33.0	40.0	27.0	41.0
38	34.61604908651711	35.0	33.0	40.0	27.0	41.0
39	34.546350067110474	35.0	33.0	40.0	26.0	41.0
40	34.37964341358647	35.0	33.0	40.0	25.0	41.0
41	34.33106949908512	35.0	33.0	39.0	26.0	41.0
42	34.263488819578335	35.0	33.0	39.0	26.0	41.0
43	34.17958145804608	35.0	33.0	39.0	26.0	41.0
44	34.09312890895619	35.0	33.0	39.0	26.0	41.0
45	34.02809004462148	35.0	33.0	39.0	26.0	41.0
46	33.97473553270684	35.0	33.0	39.0	26.0	41.0
47	33.88078327990511	35.0	33.0	39.0	25.0	41.0
48	33.76286781886628	35.0	33.0	38.0	25.0	41.0
49	33.70823228220893	35.0	33.0	38.0	24.0	40.0
50	33.560180220284224	35.0	33.0	38.0	24.0	40.0
51	33.46954350425547	35.0	32.0	38.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	6.0
13	5.0
14	18.0
15	51.0
16	157.0
17	424.0
18	990.0
19	1916.0
20	3484.0
21	5458.0
22	8471.0
23	12337.0
24	17628.0
25	23608.0
26	30743.0
27	37331.0
28	45173.0
29	53856.0
30	67100.0
31	83770.0
32	106529.0
33	154823.0
34	231890.0
35	308986.0
36	160093.0
37	200998.0
38	246286.0
39	273405.0
40	143.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.91463624967601	25.34203216099576	28.940078489865016	11.803253099463213
2	29.77749000087682	26.019833481236525	31.271215918430666	12.931460599455985
3	29.238052842391077	26.29410478098283	29.819837528099196	14.648004848526893
4	23.11278895322116	39.165825979123966	25.1876250793715	12.53375998828337
5	23.7691033597632	32.96025113673482	28.775698782376107	14.494946721125876
6	22.67712491605169	41.12792807376081	25.428702469838825	10.766244540348666
7	89.78841653008504	4.238269638605528	4.143457427486484	1.8298564038229364
8	91.86893753474762	2.8538090131339966	3.436701768382633	1.8405516837357552
9	88.36787137914189	4.192983318253953	4.966126795915752	2.4730185066884043
10	60.62677230905312	20.267989027220935	8.658840805094423	10.446397858631524
11	51.14420224292546	19.84494734742605	15.056978862850862	13.95387154679763
12	45.55967628952797	20.26582106507644	18.883913817241755	15.290588828153831
13	23.55852197012837	38.56212078728823	20.816531626713534	17.062825615869865
14	17.440918213868983	39.07852937010583	25.399796307912293	18.0807561081129
15	17.082963575345357	21.80478512604532	44.832204547710106	16.28004675089922
16	20.41121905956693	17.479989709406354	41.9780582960203	20.13073293500642
17	21.582207679210978	18.517865453378697	23.910839907076326	35.989086960334
18	25.86822066193184	22.869447246736254	29.538484218680892	21.723847872651014
19	34.01903567116736	24.52803464114445	20.251753399605526	21.201176288082664
20	36.46642404761423	23.07169402634893	21.31641552029646	19.14546640574038
21	27.47410248776065	27.175501835059514	23.91980081727355	21.430594859906286
22	26.63876258502025	23.63093190575435	22.834518967741687	26.895786541483712
23	25.207088561735368	29.22726120860517	21.930478753489215	23.635171476170242
24	24.87881091612299	22.741103887782423	33.42944632173907	18.950638874355512
25	22.748812197629505	23.45701316482968	31.13439341864505	22.659781218895766
26	20.93278257459476	33.827773233086766	23.118762893352642	22.120681298965835
27	22.224358066409017	31.54399373314409	26.030673291958976	20.200974908487908
28	18.48154004322435	27.398416520449665	34.33767792947089	19.782365506855097
29	19.20616934578611	24.996266287417825	33.62620093058571	22.171363436210363
30	23.180622079875434	28.60659773510586	27.06724825864463	21.14553192637408
31	33.03531080387073	24.400702997858055	21.282498956969324	21.281487241301893
32	34.342495623125316	25.440553996228708	21.410505077367343	18.806445303278633
33	32.07341008882864	26.158004935245376	21.301095254475396	20.467489721450587
34	22.349858986106735	26.72644460953075	25.275307103882	25.648389300480517
35	24.10634191557281	24.504283411428148	27.826083186152793	23.563291486846254
36	33.57132739986183	23.92408856462599	22.477094275520045	20.027489759992136
37	23.83982710261013	31.107751572736092	25.339767844978176	19.712653479675595
38	24.73167855191691	30.93147216192076	21.01453883591032	23.32231045025201
39	24.410145677420722	30.187572084741305	23.42487914815468	21.977403089683296
40	28.175895922400446	23.545321489515253	23.399538079532416	24.87924450855189
41	20.80034417603467	22.296334409606093	24.943560718838434	31.9597606955208
42	24.99168947844612	24.249668301791893	22.00606836692711	28.75257385283487
43	24.41501154801169	25.017030547068387	23.39708105576866	27.170876849151266
44	21.928118083598548	28.67423815401396	26.369212625055283	23.028431137332213
45	20.980814980329356	33.287372535870134	20.565626141191185	25.166186342609322
46	23.497048199097936	30.666595364800582	23.65555032032845	22.180806115773034
47	23.92057164825826	25.401097085198987	25.681775917505668	24.996555349037088
48	26.131652150955688	21.236489982569584	28.97095990618987	23.66089796028486
49	23.437212443910003	22.036997960188508	29.672897871639297	24.852891724262193
50	20.684430466709255	31.636156212753207	24.169116463889942	23.510296856647596
51	20.3455057181206	32.87223187366851	21.93124958447392	24.85101282373697
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	305.0
1	775.0
2	1245.0
3	3343.5
4	5442.0
5	4119.5
6	2797.0
7	2932.0
8	3067.0
9	3358.5
10	3650.0
11	3660.5
12	3671.0
13	3530.0
14	3389.0
15	3352.5
16	3316.0
17	3416.0
18	3516.0
19	3389.0
20	3262.0
21	3182.5
22	3103.0
23	3633.5
24	4164.0
25	4922.5
26	7143.0
27	8605.0
28	11632.0
29	14659.0
30	17661.5
31	20664.0
32	23792.5
33	26921.0
34	31380.5
35	35840.0
36	37392.5
37	38945.0
38	51976.0
39	65007.0
40	96418.0
41	127829.0
42	156725.5
43	185622.0
44	189839.5
45	194057.0
46	174111.0
47	154165.0
48	149411.0
49	144657.0
50	139602.0
51	134547.0
52	128483.5
53	122420.0
54	117809.5
55	113199.0
56	111454.0
57	109709.0
58	114684.0
59	119659.0
60	114487.0
61	109315.0
62	100187.0
63	91059.0
64	79598.5
65	68138.0
66	57553.0
67	46968.0
68	40173.0
69	33378.0
70	29617.0
71	25856.0
72	20482.0
73	15108.0
74	12465.0
75	8142.0
76	6462.0
77	4835.5
78	3209.0
79	2693.0
80	2177.0
81	1397.0
82	617.0
83	441.0
84	265.0
85	156.0
86	47.0
87	39.5
88	32.0
89	70.5
90	109.0
91	57.0
92	5.0
93	3.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2075682.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.528079569958894
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.20202735052604	13.09057410151555
2	8.079024100940751	2.6706150637592874
3	2.638194357106383	1.3081285876581255
4	1.2576553558876582	0.8314651117478474
5	0.7377612358266046	0.6096888204686665
6	0.48091386457059254	0.4769149571951513
7	0.37190427630016587	0.43028044297679846
8	0.3016399343822441	0.39884230695575285
9	0.23094105326794787	0.3435310893944482
>10	3.246572934505909	13.403359796027248
>50	1.666791579528212	20.160925394405304
>100	1.7779953519924094	45.13906076063371
>500	0.007307700696178194	0.7943796632773056
>1k	0.0012709044689005555	0.34223390398480863
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2449	0.11798531759681877	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05198291453122395	0.0
2	0.0	0.0	0.0	0.1576349363727199	0.0
3	0.0	0.0	0.0	0.22089125405529364	0.0
4	0.0	0.0	0.0	0.3742866200121213	0.0
5	0.0	0.0	0.0	0.6231204972630683	0.0
6	0.0	0.0	0.0	0.8879009405101552	0.0
7	0.0	0.0	0.0	1.021591939420393	0.0
8	0.0	0.0	0.0	1.3521820779868978	0.0
9	0.0	0.0	0.0	1.4429474264362268	0.0
10	0.0	0.0	0.0	1.6099286884985273	0.0
11	0.0	0.0	0.0	1.8829955648312218	0.0
12	0.0	0.0	0.0	2.096901163087602	0.0
13	0.0	0.0	0.0	2.19335139004915	0.0
14	0.0	0.0	0.0	2.232326531713432	0.0
15	0.0	0.0	0.0	2.292692233203352	0.0
16	0.0	0.0	0.0	2.4258532858116033	0.0
17	4.81769365442298E-5	0.0	0.0	2.563735678201189	0.0
18	9.63538730884596E-5	0.0	0.0	2.7298015784691487	0.0
19	9.63538730884596E-5	0.0	0.0	2.8372361469627814	0.0
20	9.63538730884596E-5	0.0	0.0	2.945971492743108	0.0
21	9.63538730884596E-5	0.0	0.0	3.09690983493618	0.0
22	9.63538730884596E-5	0.0	0.0	3.2424523602362982	0.0
23	9.63538730884596E-5	0.0	0.0	3.401532604705345	0.0
24	9.63538730884596E-5	0.0	0.0	3.519999691667606	0.0
25	9.63538730884596E-5	0.0	0.0	3.6200631888699712	0.0
26	9.63538730884596E-5	0.0	0.0	3.7239808409958752	0.0
27	9.63538730884596E-5	0.0	0.0	3.8320417096645825	0.0
28	9.63538730884596E-5	0.0	0.0	3.9396208089678475	0.0
29	1.445308096326894E-4	0.0	0.0	4.063146474267253	0.0
30	1.927077461769192E-4	0.0	0.0	4.219480633353278	0.0
31	1.927077461769192E-4	0.0	0.0	4.361602596158757	0.0
32	1.927077461769192E-4	0.0	0.0	4.486958985046843	0.0
33	1.927077461769192E-4	0.0	0.0	4.613567974285078	0.0
34	1.927077461769192E-4	0.0	0.0	4.754533690613495	0.0
35	1.927077461769192E-4	0.0	0.0	4.938665942085541	0.0
36	2.40884682721149E-4	0.0	0.0	5.080498843271753	0.0
37	2.40884682721149E-4	0.0	0.0	5.24584208949155	0.0
38	2.890616192653788E-4	0.0	0.0	5.4291553330423445	0.0
39	2.890616192653788E-4	0.0	0.0	5.735319764780925	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTTACG	25	3.8918955E-5	45.000004	1
TCGATCT	25	3.8918955E-5	45.000004	15
ATCGTAC	25	3.8918955E-5	45.000004	16
CACCGTA	25	3.8918955E-5	45.000004	34
TACGTCA	25	3.8918955E-5	45.000004	32
CGCTTAT	25	3.8918955E-5	45.000004	33
ATCGTTC	20	7.0346025E-4	45.0	16
CATTACG	20	7.0346025E-4	45.0	12
CCGTACT	40	6.8193913E-9	45.0	33
TATAGCG	125	0.0	44.999996	1
ATACGAC	30	2.166351E-6	44.999996	22
ACGTTAG	65	0.0	41.53846	1
CGGGTAT	185	0.0	41.351353	6
TATCGTG	55	6.184564E-11	40.909092	1
GTACTAT	105	0.0	40.714287	39
CCGCAAT	50	1.0822987E-9	40.500004	33
TAATACG	45	1.929584E-8	40.0	1
CTTACCG	45	1.929584E-8	40.0	1
CGTATTT	395	0.0	39.873417	1
ATAGGGA	1725	0.0	39.782608	4
>>END_MODULE