##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545272_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3174527
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.268294142717956	31.0	31.0	33.0	30.0	34.0
2	31.5675711688702	31.0	31.0	34.0	30.0	34.0
3	31.64682014044927	31.0	31.0	34.0	30.0	34.0
4	30.268196490374788	35.0	33.0	37.0	0.0	37.0
5	32.817351687353735	35.0	33.0	37.0	19.0	37.0
6	34.43777419439179	35.0	33.0	37.0	30.0	37.0
7	35.50098991125292	36.0	35.0	37.0	33.0	37.0
8	35.69881812314087	37.0	35.0	37.0	35.0	37.0
9	37.73173704303035	39.0	37.0	39.0	35.0	39.0
10	37.144002240333755	39.0	37.0	39.0	33.0	39.0
11	36.65943461813366	39.0	35.0	39.0	32.0	39.0
12	35.43793957336006	37.0	35.0	39.0	31.0	39.0
13	34.933418427375166	35.0	34.0	39.0	30.0	39.0
14	35.70984748279035	37.0	34.0	40.0	30.0	41.0
15	36.07512111253109	37.0	35.0	40.0	31.0	41.0
16	36.277391876018065	37.0	35.0	40.0	31.0	41.0
17	36.21791939397586	36.0	35.0	40.0	31.0	41.0
18	36.22415937870429	36.0	35.0	40.0	31.0	41.0
19	36.21309347817801	36.0	35.0	40.0	31.0	41.0
20	36.10532151718981	36.0	34.0	40.0	31.0	41.0
21	35.92745438926807	36.0	34.0	40.0	31.0	41.0
22	35.79516192491039	36.0	34.0	40.0	30.0	41.0
23	35.75558406023953	36.0	34.0	40.0	30.0	41.0
24	35.68052909929574	35.0	34.0	40.0	30.0	41.0
25	35.563128302263614	35.0	34.0	40.0	30.0	41.0
26	35.42029883507055	35.0	34.0	40.0	30.0	41.0
27	35.40987271489579	35.0	34.0	40.0	30.0	41.0
28	35.45187046763187	36.0	34.0	40.0	30.0	41.0
29	35.551195500936046	36.0	34.0	40.0	30.0	41.0
30	35.48330160682205	36.0	34.0	40.0	30.0	41.0
31	35.344711826360275	36.0	34.0	40.0	30.0	41.0
32	35.20657565678288	35.0	34.0	40.0	29.0	41.0
33	35.068822221389205	35.0	34.0	40.0	29.0	41.0
34	34.99198179760323	35.0	34.0	40.0	29.0	41.0
35	34.9089442301168	35.0	34.0	40.0	29.0	41.0
36	34.71569528310832	35.0	33.0	40.0	27.0	41.0
37	34.60693797847679	35.0	33.0	40.0	27.0	41.0
38	34.60692474815933	35.0	33.0	40.0	27.0	41.0
39	34.59383366403877	35.0	33.0	40.0	27.0	41.0
40	34.49224719147136	35.0	33.0	40.0	27.0	41.0
41	34.498792733531644	35.0	33.0	40.0	27.0	41.0
42	34.43625617296687	35.0	33.0	39.0	27.0	41.0
43	34.33863060544138	35.0	33.0	39.0	27.0	41.0
44	34.263936958167314	35.0	33.0	39.0	27.0	41.0
45	34.15799204101902	35.0	33.0	39.0	26.0	41.0
46	34.103923198637155	35.0	33.0	39.0	26.0	41.0
47	34.032931835199385	35.0	33.0	39.0	26.0	41.0
48	33.96814454562837	35.0	33.0	39.0	26.0	41.0
49	33.93287094423831	35.0	33.0	39.0	26.0	41.0
50	33.757972762556435	35.0	33.0	38.0	25.0	40.0
51	33.66837957276785	35.0	33.0	38.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	6.0
13	7.0
14	20.0
15	76.0
16	211.0
17	523.0
18	1341.0
19	2759.0
20	4828.0
21	7800.0
22	12490.0
23	18185.0
24	25705.0
25	35032.0
26	45689.0
27	56711.0
28	68175.0
29	83621.0
30	103479.0
31	130227.0
32	167011.0
33	245596.0
34	373740.0
35	513083.0
36	224701.0
37	272574.0
38	351218.0
39	429387.0
40	330.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.3226105810409	25.2350980161769	30.017385267159487	12.424906135622724
2	26.920199450185805	27.22597728732501	31.970085622204504	13.88373764028468
3	28.05047807122132	26.12789243877907	31.323154599094604	14.498474890905008
4	22.313402910102827	38.35973044173195	26.980491896903064	12.346374751262157
5	23.589467029261368	32.252804906053726	29.76226064544419	14.395467419240724
6	21.074100173033653	41.68381620316979	25.86312858577042	11.378955038026138
7	89.71727126592403	4.435999441806606	4.259973218057367	1.5867560742120008
8	91.99077531865377	2.4739748630268386	3.471950309447675	2.063299508871715
9	88.96821479231394	4.065172543815189	4.704574886274396	2.2620377775964733
10	61.020901696536214	21.86234358693437	8.317554079710144	8.79920063681928
11	53.560798191352596	18.4154363783959	16.2273623755602	11.796403054691297
12	48.02409933826362	21.67538660090149	17.56041136207063	12.740102698764257
13	21.111460069484366	45.88267795485753	18.316177496679035	14.689684478979073
14	13.972947780882002	45.80121700020192	24.6022478309367	15.623587387979374
15	14.589890084412577	22.83288187500059	49.13163441356775	13.44559362701908
16	16.040058881212854	18.06083236967271	46.67227590126025	19.226832847854183
17	16.10526544584437	19.646958428767498	25.61991124977044	38.62786487561769
18	22.495256773686283	24.33691696432256	31.757865029971395	21.40996123201976
19	32.43302073033242	25.763806702541824	20.369585768210506	21.43358679891524
20	35.756413475141336	24.299683070895288	21.268774844252388	18.67512860971099
21	23.884251102605205	30.287945259246495	24.422347014216605	21.40545662393169
22	23.91836642120228	26.588464990217442	21.68285228004046	27.81031630853982
23	22.07572970713432	32.09492311768021	21.701343223730653	24.128003951454815
24	22.15268605370186	23.385027123725834	37.29172881503292	17.17055800753939
25	17.513160228279677	26.59832472680182	34.168428871450764	21.72008617346773
26	17.075835234666457	38.94589650678668	23.914617831254862	20.063650427292004
27	19.6974541404121	35.46720503558483	26.66850841085932	18.166832413143755
28	14.879980545133181	29.611529528651044	38.39608231399512	17.112407612220657
29	16.667238930398135	24.668462419755762	36.944905493007305	21.719393156838798
30	20.909634726685265	31.190567917677186	28.22014114228671	19.679656213350842
31	33.136023098874254	27.40653961991818	20.033630206956815	19.423807074250746
32	35.00178136774392	27.472565204202077	21.304654205177652	16.220999222876355
33	30.177031097861196	28.884145575073074	21.220799193076637	19.718024133989097
34	19.76262920428776	28.06528342647582	26.39816892406333	25.773918445173095
35	21.5957526900858	25.533914186270902	28.798526520643865	24.071806602999438
36	32.95237369220674	25.806616229756436	23.008656092702946	18.232353985333877
37	22.739734139920685	33.058657242480535	26.607333942977963	17.594274674620817
38	20.909067713079775	35.70213767279346	21.79622350038289	21.592571113743876
39	22.221231698454606	33.5150716941453	24.39484685435027	19.868849753049826
40	26.570982070714788	26.079507277777132	22.29916456845382	25.050346083054265
41	17.894980890066456	24.399162457903177	24.968066108746278	32.737790543284085
42	22.823274144463095	26.446837591868018	21.42624712279971	29.303641140869175
43	22.374577377984185	27.45716133458622	23.703184757918265	26.46507652951133
44	20.83072533325437	30.997121775937014	26.207211342036153	21.96494154877246
45	18.470247693593407	37.62774737779833	20.191385992306884	23.710618936301376
46	22.37646742333582	34.316072914169574	22.83918202617272	20.468277636321883
47	21.774172971280446	25.868357711243284	26.76556223966594	25.591907077810333
48	24.510864138184996	20.91013873877904	31.50248840220921	23.076508720826755
49	20.49001315786572	23.175484095740877	31.855863881453832	24.478638864939565
50	18.902879074583396	34.19690555474879	25.354706386179735	21.54550898448808
51	17.915298877596566	34.42859361410377	22.574607177699228	25.08150033060043
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	550.0
1	978.5
2	1407.0
3	4600.5
4	7794.0
5	5749.0
6	3704.0
7	4093.0
8	4482.0
9	5045.0
10	5608.0
11	5884.0
12	6160.0
13	6361.0
14	6562.0
15	6262.5
16	5963.0
17	5967.0
18	5971.0
19	5518.0
20	5065.0
21	5150.5
22	5236.0
23	6380.5
24	7525.0
25	9823.5
26	14437.0
27	16752.0
28	25197.0
29	33642.0
30	40193.5
31	46745.0
32	49612.5
33	52480.0
34	61728.5
35	70977.0
36	76928.5
37	82880.0
38	108576.0
39	134272.0
40	188566.0
41	242860.0
42	299494.0
43	356128.0
44	345432.0
45	334736.0
46	309344.0
47	283952.0
48	271890.0
49	259828.0
50	245749.5
51	231671.0
52	209445.5
53	187220.0
54	173315.0
55	159410.0
56	146139.0
57	132868.0
58	128675.5
59	124483.0
60	120158.5
61	115834.0
62	95456.5
63	75079.0
64	64185.0
65	53291.0
66	43875.0
67	34459.0
68	28323.5
69	22188.0
70	19806.0
71	17424.0
72	15298.5
73	13173.0
74	10307.0
75	5269.5
76	3098.0
77	2226.5
78	1355.0
79	1243.0
80	1131.0
81	892.0
82	653.0
83	436.5
84	220.0
85	165.5
86	111.0
87	61.0
88	11.0
89	7.5
90	4.0
91	2.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3174527.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.510980682956482
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.29273421208163	11.833751266135312
2	10.38443772178616	3.221456258119795
3	3.9198562054641326	1.8240244164876376
4	1.9048420163707218	1.1818387088004059
5	1.102562783574959	0.8550915018888959
6	0.6868905671551596	0.6392607791069223
7	0.47716388189290815	0.5180895828251799
8	0.3285120167361214	0.4076434836570445
9	0.26321051122226824	0.36743878396077334
>10	2.068868767965614	7.147172425790357
>50	0.7606592339783791	8.647486867928752
>100	1.7695073747698427	58.46631050941401
>500	0.03632484754541384	3.5579483413058957
>1k	0.004208366483919896	1.1353882387697924
>5k	2.2149297283788927E-4	0.1970988358091957
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	5737	0.18071983637247374	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3944	0.12423898111435185	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3561	0.11217419161972791	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0883281194332258	0.0
2	0.0	0.0	0.0	0.2931775347949474	0.0
3	0.0	0.0	0.0	0.408501802000739	0.0
4	0.0	0.0	0.0	0.6485060609029314	0.0
5	3.150075586063688E-5	0.0	0.0	1.0546768069699832	0.0
6	3.150075586063688E-5	0.0	0.0	1.4628005999003946	0.0
7	3.150075586063688E-5	0.0	0.0	1.676942738241004	0.0
8	3.150075586063688E-5	0.0	0.0	2.218440731485352	0.0
9	3.150075586063688E-5	0.0	0.0	2.3745584775306683	0.0
10	6.300151172127376E-5	0.0	0.0	2.652899156315256	0.0
11	6.300151172127376E-5	0.0	0.0	3.0980993388936366	0.0
12	9.450226758191063E-5	0.0	0.0	3.4444186488254784	0.0
13	9.450226758191063E-5	0.0	0.0	3.583179478391584	0.0
14	9.450226758191063E-5	0.0	0.0	3.635376230852659	0.0
15	9.450226758191063E-5	0.0	0.0	3.7160181658558895	0.0
16	9.450226758191063E-5	0.0	0.0	3.9010851065371313	0.0
17	9.450226758191063E-5	0.0	0.0	4.13891581328494	0.0
18	1.260030234425475E-4	0.0	0.0	4.407081747926542	0.0
19	1.260030234425475E-4	0.0	0.0	4.583548982257829	0.0
20	1.5750377930318437E-4	0.0	0.0	4.752550537450146	0.0
21	1.5750377930318437E-4	0.0	0.0	4.980206500054969	0.0
22	1.5750377930318437E-4	0.0	0.0	5.224998873847978	0.0
23	1.5750377930318437E-4	0.0	0.0	5.495495864423267	0.0
24	1.5750377930318437E-4	0.0	0.0	5.706739933224697	0.0
25	1.5750377930318437E-4	0.0	0.0	5.869063328174559	0.0
26	1.5750377930318437E-4	0.0	0.0	6.024992069684712	0.0
27	1.5750377930318437E-4	0.0	0.0	6.167942499780282	0.0
28	1.8900453516382127E-4	0.0	0.0	6.330580902288751	0.0
29	1.8900453516382127E-4	0.0	0.0	6.518766417800195	0.0
30	1.8900453516382127E-4	0.0	0.0	6.758518670655502	0.0
31	1.8900453516382127E-4	0.0	0.0	6.971148772714801	0.0
32	1.8900453516382127E-4	0.0	0.0	7.153853156706496	0.0
33	2.2050529102445813E-4	0.0	0.0	7.34109364954212	0.0
34	2.2050529102445813E-4	0.0	0.0	7.545061043739745	0.0
35	2.2050529102445813E-4	0.0	0.0	7.799524149581969	0.0
36	2.52006046885095E-4	0.0	0.0	8.016595858217618	0.0
37	3.1500755860636875E-4	0.0	0.0	8.233604565341546	0.0
38	3.1500755860636875E-4	0.0	0.0	8.442612080476872	0.0
39	3.1500755860636875E-4	0.0	0.0	8.651651096368058	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTCG	75	0.0	45.000004	1
CGAGTTA	20	7.035166E-4	45.000004	38
CACGACT	35	1.2128294E-7	45.000004	20
CGACTAG	20	7.035166E-4	45.000004	30
CAATCTA	40	6.8212103E-9	45.000004	42
AGTACGT	20	7.035166E-4	45.000004	40
CCCGACA	20	7.035166E-4	45.000004	27
TGACGTA	25	3.8923645E-5	45.0	42
ACTTACG	45	3.8562575E-10	45.0	15
ATCGTGT	50	2.1827873E-11	45.0	11
TACGCTA	25	3.8923645E-5	45.0	1
TACGATC	25	3.8923645E-5	45.0	11
CTACGAT	25	3.8923645E-5	45.0	34
ACCTTCG	45	3.8562575E-10	45.0	32
TATCCGT	25	3.8923645E-5	45.0	27
CCCACTA	55	1.8189894E-12	44.999996	32
CTCGATG	30	2.1667165E-6	44.999996	1
CGTAAGT	30	2.1667165E-6	44.999996	18
TATCGTG	65	0.0	44.999996	1
TCGATAG	135	0.0	43.333332	1
>>END_MODULE