FastQCFastQC Report
Thu 26 May 2016
SRR1545271_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545271_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2077513
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33400.16076915042168208No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACCG351.2125565E-745.035
GTCGAAC253.8918974E-545.018
ACGCATA207.0346036E-445.031
TTATCCG351.2125565E-745.01
TGTAACG351.2125565E-745.01
ATCGCAA351.2125565E-745.014
CCCGCTA207.0346036E-445.016
CTACGAT302.1663527E-644.99999616
CGTCGGT302.1663527E-644.99999617
CGGTCTA1100.042.95454831
TCGATAG1400.041.7857131
CGTTTTT26550.040.5932241
CCGAACA403.460027E-739.37537
ACTCACG403.460027E-739.37539
CGATACG403.460027E-739.37510
ATAGGGC14650.039.3174064
CGTTGAT2250.039.025
CGTATTT5250.038.9999961
GCGCGAC5750.038.7391329
TACGATA356.250726E-638.57142617
Produced by FastQC (version 0.11.5)