##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545271_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2077513
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.266802662606683	31.0	31.0	33.0	30.0	34.0
2	31.6018099525731	31.0	31.0	34.0	30.0	34.0
3	31.714594325041528	31.0	31.0	34.0	30.0	34.0
4	30.328689158623796	35.0	33.0	37.0	0.0	37.0
5	32.894743859605214	35.0	33.0	37.0	19.0	37.0
6	34.534713380854896	35.0	33.0	37.0	30.0	37.0
7	35.49364745250691	36.0	35.0	37.0	33.0	37.0
8	35.67245981132248	37.0	35.0	37.0	35.0	37.0
9	37.72547079127784	39.0	37.0	39.0	35.0	39.0
10	37.12933878151424	39.0	37.0	39.0	33.0	39.0
11	36.6958483532955	39.0	35.0	39.0	32.0	39.0
12	35.742030976460796	37.0	35.0	39.0	31.0	39.0
13	35.364761616413475	37.0	35.0	39.0	30.0	39.0
14	36.16943865092541	38.0	35.0	40.0	30.0	41.0
15	36.484542816338575	38.0	35.0	40.0	31.0	41.0
16	36.64563157968205	38.0	35.0	40.0	32.0	41.0
17	36.60498442127679	38.0	35.0	40.0	32.0	41.0
18	36.55163168654059	38.0	35.0	40.0	31.0	41.0
19	36.55045239187432	38.0	35.0	40.0	31.0	41.0
20	36.42626207393167	38.0	35.0	40.0	31.0	41.0
21	36.25501549208116	38.0	35.0	40.0	31.0	41.0
22	36.1675440779432	37.0	35.0	40.0	30.0	41.0
23	36.12738933522919	37.0	34.0	40.0	31.0	41.0
24	36.06799042894076	37.0	34.0	40.0	30.0	41.0
25	35.94741452881402	37.0	34.0	40.0	30.0	41.0
26	35.821380660433896	37.0	34.0	40.0	30.0	41.0
27	35.813826435743124	37.0	34.0	40.0	30.0	41.0
28	35.824478595320464	37.0	34.0	40.0	30.0	41.0
29	35.82439291595287	37.0	34.0	40.0	30.0	41.0
30	35.77509599217911	37.0	34.0	40.0	30.0	41.0
31	35.62794264103281	36.0	34.0	40.0	30.0	41.0
32	35.54479033344196	36.0	34.0	40.0	29.0	41.0
33	35.41375769971115	36.0	34.0	40.0	29.0	41.0
34	35.29970642782981	36.0	34.0	40.0	29.0	41.0
35	35.170570773805025	36.0	34.0	40.0	28.0	41.0
36	35.00630994848167	36.0	34.0	40.0	27.0	41.0
37	34.959092434078634	36.0	34.0	40.0	27.0	41.0
38	34.920203628088004	36.0	33.0	40.0	27.0	41.0
39	34.867031878982225	36.0	34.0	40.0	27.0	41.0
40	34.7611369940886	36.0	34.0	40.0	27.0	41.0
41	34.718152906865086	36.0	34.0	40.0	26.0	41.0
42	34.64605227500382	35.0	33.0	40.0	26.0	41.0
43	34.56506361211699	35.0	33.0	40.0	26.0	41.0
44	34.49617210578225	35.0	33.0	40.0	26.0	41.0
45	34.40139628488486	35.0	33.0	40.0	26.0	41.0
46	34.36471492597158	35.0	33.0	40.0	26.0	41.0
47	34.30093481966178	35.0	33.0	39.0	26.0	41.0
48	34.18959688820238	35.0	33.0	39.0	26.0	41.0
49	34.12623507049053	35.0	33.0	39.0	26.0	41.0
50	33.97251377007027	35.0	33.0	39.0	25.0	41.0
51	33.88361420602422	35.0	33.0	39.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	3.0
13	10.0
14	10.0
15	52.0
16	118.0
17	379.0
18	888.0
19	1772.0
20	3151.0
21	4990.0
22	7812.0
23	11664.0
24	16378.0
25	22416.0
26	29216.0
27	35989.0
28	43067.0
29	52029.0
30	63760.0
31	79107.0
32	101403.0
33	146337.0
34	218356.0
35	290959.0
36	159393.0
37	201064.0
38	262715.0
39	324345.0
40	127.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.6939951759628	24.373902834783706	30.772900097376045	12.159201891877451
2	28.337151199535214	26.35906490115826	31.558502883014448	13.745281016292077
3	29.633942122143157	25.594881957417353	31.080238727748032	13.690937192691454
4	22.096371960127325	38.72442675448962	26.085661076489053	13.093540208894
5	22.89809979528407	32.590842993521576	29.822099789507934	14.68895742168641
6	21.942534174274723	40.880803152615655	26.389774696957375	10.786887976152256
7	90.40703957087152	3.80113144899695	4.166279585254099	1.6255493948774329
8	92.69506376133386	2.1600346183152643	3.582793465070977	1.5621081552798948
9	89.4918106408961	3.498076787004462	4.802232284467053	2.207880287632376
10	57.40493561291795	20.646224596428517	11.039882782923621	10.908957007729915
11	47.37919810850762	21.34658122476249	17.859094022516345	13.415126644213537
12	41.724696788900964	22.43355396572729	20.64068913166849	15.20106011370326
13	21.985807068355285	38.57511360939739	22.519088929888767	16.919990392358557
14	16.158117903474007	39.670991228454405	27.147892696700332	17.02299817137125
15	16.330728135034533	23.000241153725632	44.70956379093657	15.959466920303267
16	18.861446354366976	19.357568400294006	41.70934189100141	20.071643354337613
17	19.600936311830537	20.137202510886816	26.554153933092117	33.70770724419053
18	24.175251851612963	23.24885572316515	31.06579838489579	21.510094040326102
19	31.400477397734694	24.91950712221777	22.933815576605294	20.746199903442243
20	33.286578712142834	24.711229243812195	23.109073204355397	18.89311883968957
21	24.96335763001242	27.062357732538857	27.128157561468928	20.84612707597979
22	24.99286406390718	24.47633300008231	24.910698513077897	25.62010442293261
23	22.147442639348107	29.010793193592534	25.01505405742347	23.826710109635897
24	22.394613174502396	23.497422158128494	35.2268313122469	18.881133355122206
25	20.2879115557881	25.817503909722827	31.961821658877703	21.932762875611367
26	18.864912036651514	34.98818057937544	24.863671129855746	21.283236254117302
27	19.203153000727312	33.12417298953123	28.453299690543453	19.219374319198003
28	18.02742028569737	27.85099299017623	35.92367412382016	18.197912600306232
29	18.03377403655236	25.273728732383383	35.511739276721734	21.180757954342525
30	21.563234502022368	29.142970465166762	28.83880871022227	20.454986322588596
31	30.15056945492038	26.18592519035982	23.21362128660567	20.449884068114134
32	32.09582804054656	25.728695801181512	23.833882146585847	18.341594011686087
33	27.95770712385434	26.646523992870318	24.538089533013753	20.857679350261588
34	20.373687192330443	27.097014555384252	28.16444469902234	24.364853553262964
35	22.15678072772589	25.183091513747446	29.63004322957305	23.03008452895361
36	30.56495916030369	24.73688491961302	24.87445325251876	19.823702667564536
37	21.911054226856823	30.573382693634166	27.291092763318446	20.22447031619056
38	22.11658844012047	32.246344547543146	22.888761706906287	22.748305305430097
39	22.203711841995695	29.742389096963535	25.55651877990655	22.497380281134223
40	25.09360952253969	24.819050470442303	24.796619804545145	25.290720202472862
41	19.80974366947403	23.221467206222055	27.11809745594853	29.850691668355385
42	22.83773916216168	25.806866190488336	24.198308265700383	27.1570863816496
43	23.059542828372194	26.05947592145031	25.6862411931959	25.19474005698159
44	21.152406747875947	29.527276122941227	27.25013032409424	22.070186805088586
45	19.573547794887443	34.27343174266539	22.44650213981814	23.706518322629027
46	22.57569507386957	30.91075723713883	24.69813666629282	21.815411022698775
47	21.6585407648472	27.01619676988784	26.701156623327986	24.62410584193697
48	23.963412021970502	23.160336421480878	30.177380358149385	22.69887119839924
49	21.828744272599014	23.700982857869	30.19470876957208	24.275564099959904
50	20.210848259433277	31.66040356907514	26.117526099716343	22.011222071775244
51	19.598000108783918	33.06491944936084	23.593691110476804	23.74338933137843
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	312.0
1	718.5
2	1125.0
3	3907.0
4	6689.0
5	4875.0
6	3061.0
7	3305.0
8	3549.0
9	3821.5
10	4094.0
11	4131.0
12	4168.0
13	4070.0
14	3972.0
15	3854.5
16	3737.0
17	3639.0
18	3541.0
19	3940.5
20	4340.0
21	4406.0
22	4472.0
23	5991.5
24	7511.0
25	8849.5
26	13118.5
27	16049.0
28	20132.0
29	24215.0
30	26481.0
31	28747.0
32	35235.5
33	41724.0
34	49237.0
35	56750.0
36	56984.5
37	57219.0
38	72080.5
39	86942.0
40	118393.0
41	149844.0
42	174537.0
43	199230.0
44	195738.5
45	192247.0
46	183303.0
47	174359.0
48	165882.5
49	157406.0
50	150252.0
51	143098.0
52	132921.0
53	122744.0
54	111511.0
55	100278.0
56	95693.5
57	91109.0
58	89015.5
59	86922.0
60	83517.5
61	80113.0
62	71881.5
63	63650.0
64	55392.5
65	47135.0
66	40645.0
67	34155.0
68	29100.0
69	24045.0
70	19985.5
71	15926.0
72	13540.5
73	11155.0
74	8650.0
75	4659.5
76	3174.0
77	2230.5
78	1287.0
79	1018.0
80	749.0
81	504.5
82	260.0
83	155.5
84	51.0
85	35.5
86	20.0
87	12.5
88	5.0
89	3.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2077513.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.432583011956478
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.56726761948583	14.481976824187731
2	8.902310854747727	3.2818516765675736
3	3.015152349448647	1.6673113792472338
4	1.3929132098571348	1.0269995347656957
5	0.7720402187751779	0.7115347710571254
6	0.4969007026059427	0.5495498069690123
7	0.34939762688817255	0.45082105332577865
8	0.25831223948563237	0.3809089437858641
9	0.21477299176840384	0.3562938899547614
>10	2.935173953661578	13.675809569885503
>50	1.5525679764009168	21.02616850030342
>100	1.5400652397863486	41.795272685567944
>500	0.0019886472382019136	0.2270571505418615
>1k	0.0011363698504010936	0.3684442138405299
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3340	0.16076915042168208	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.058146447218380824	0.0
2	0.0	0.0	0.0	0.17785688946350756	0.0
3	0.0	0.0	0.0	0.24741120753516344	0.0
4	0.0	0.0	0.0	0.3721757697785766	0.0
5	0.0	0.0	0.0	0.6124149403637907	0.0
6	0.0	0.0	0.0	0.8604518960892182	0.0
7	0.0	0.0	0.0	0.9949877569959851	0.0
8	0.0	0.0	0.0	1.3174887473628323	0.0
9	0.0	0.0	0.0	1.407644621237027	0.0
10	0.0	0.0	0.0	1.566825333944962	0.0
11	0.0	0.0	0.0	1.8319018942360408	0.0
12	0.0	0.0	0.0	2.02448793340884	0.0
13	0.0	0.0	0.0	2.109300880427704	0.0
14	0.0	0.0	0.0	2.147327116605287	0.0
15	0.0	0.0	0.0	2.199986233539814	0.0
16	0.0	0.0	0.0	2.32802394016307	0.0
17	0.0	0.0	0.0	2.476566933636516	0.0
18	0.0	0.0	0.0	2.6425827419611814	0.0
19	0.0	0.0	0.0	2.7478528413540615	0.0
20	0.008953012568393074	0.0	0.0	2.854278168175121	0.0
21	0.008953012568393074	0.0	0.0	3.001136454982472	0.0
22	0.008953012568393074	0.0	0.0	3.1491018347418285	0.0
23	0.00900114704456723	0.0	0.0	3.3175243668752015	0.0
24	0.00900114704456723	0.0	0.0	3.4442143081655807	0.0
25	0.00900114704456723	0.0	0.0	3.555693754984927	0.0
26	0.00900114704456723	0.0	0.0	3.657786978950312	0.0
27	0.00900114704456723	0.0	0.0	3.7684962741508716	0.0
28	0.00900114704456723	0.0	0.0	3.875162273352802	0.0
29	0.00900114704456723	0.0	0.0	4.001707811214659	0.0
30	0.00900114704456723	0.0	0.0	4.146496315546521	0.0
31	0.00900114704456723	0.0	0.0	4.289937054545507	0.0
32	0.00900114704456723	0.0	0.0	4.409984438123853	0.0
33	0.00900114704456723	0.0	0.0	4.541006482269906	0.0
34	0.00900114704456723	0.0	0.0	4.6825699766981	0.0
35	0.00900114704456723	0.0	0.0	4.857490663114984	0.0
36	0.00900114704456723	0.0	0.0	5.008440380397138	0.0
37	0.00900114704456723	0.0	0.0	5.160978535393039	0.0
38	0.00900114704456723	0.0	0.0	5.331422715525727	0.0
39	0.00900114704456723	0.0	0.0	5.588797759628941	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACCG	35	1.2125565E-7	45.0	35
GTCGAAC	25	3.8918974E-5	45.0	18
ACGCATA	20	7.0346036E-4	45.0	31
TTATCCG	35	1.2125565E-7	45.0	1
TGTAACG	35	1.2125565E-7	45.0	1
ATCGCAA	35	1.2125565E-7	45.0	14
CCCGCTA	20	7.0346036E-4	45.0	16
CTACGAT	30	2.1663527E-6	44.999996	16
CGTCGGT	30	2.1663527E-6	44.999996	17
CGGTCTA	110	0.0	42.954548	31
TCGATAG	140	0.0	41.785713	1
CGTTTTT	2655	0.0	40.593224	1
CCGAACA	40	3.460027E-7	39.375	37
ACTCACG	40	3.460027E-7	39.375	39
CGATACG	40	3.460027E-7	39.375	10
ATAGGGC	1465	0.0	39.317406	4
CGTTGAT	225	0.0	39.0	25
CGTATTT	525	0.0	38.999996	1
GCGCGAC	575	0.0	38.739132	9
TACGATA	35	6.250726E-6	38.571426	17
>>END_MODULE