FastQCFastQC Report
Thu 26 May 2016
SRR1545270_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545270_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2472328
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT29750.1203319300675315No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29120.11778372448963083No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC25480.10306075892842698No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATCG207.034863E-445.00000416
CGTGCGC207.034863E-445.00000427
CGACACT207.034863E-445.00000440
ACTTACG406.8212103E-945.00000438
CTACGTT207.034863E-445.00000430
GCGTGTT207.034863E-445.00000436
ATACGTC406.8212103E-945.00000420
ATCGGCC406.8212103E-945.00000430
CTTACGA207.034863E-445.00000431
CGAACGC253.8921142E-545.024
GACACGC351.2126839E-745.033
CGACATA253.8921142E-545.023
TCTAGCG453.8562575E-1045.01
TAGCCGT351.2126839E-745.044
ATATACG502.1827873E-1145.01
TATTACG1000.045.01
CGAATCC453.8562575E-1045.028
TCATCGG351.2126839E-745.02
CCGCGAA253.8921142E-545.033
CTATGCG1300.044.9999961