##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545270_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2472328
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.275347364912747	31.0	31.0	33.0	30.0	34.0
2	31.59282667995509	31.0	31.0	34.0	30.0	34.0
3	31.691126743700675	31.0	31.0	34.0	30.0	34.0
4	30.30321219514563	35.0	33.0	37.0	0.0	37.0
5	32.871782384861554	35.0	33.0	37.0	19.0	37.0
6	34.48939784688763	35.0	33.0	37.0	30.0	37.0
7	35.49787083267269	36.0	35.0	37.0	33.0	37.0
8	35.6854770079051	37.0	35.0	37.0	35.0	37.0
9	37.73171884960248	39.0	37.0	39.0	35.0	39.0
10	37.15282964072728	39.0	37.0	39.0	33.0	39.0
11	36.70463870489676	39.0	35.0	39.0	32.0	39.0
12	35.56496710792419	37.0	35.0	39.0	31.0	39.0
13	35.09220216734996	37.0	35.0	39.0	30.0	39.0
14	35.90180833611074	37.0	35.0	40.0	30.0	41.0
15	36.267173287686745	37.0	35.0	40.0	31.0	41.0
16	36.46178783721254	37.0	35.0	40.0	32.0	41.0
17	36.408667862840204	37.0	35.0	40.0	32.0	41.0
18	36.391082817490236	37.0	35.0	40.0	31.0	41.0
19	36.36265819098437	37.0	35.0	40.0	31.0	41.0
20	36.254520840276854	37.0	35.0	40.0	31.0	41.0
21	36.0806717393485	37.0	34.0	40.0	31.0	41.0
22	35.930898731883474	37.0	34.0	40.0	30.0	41.0
23	35.906819807080616	36.0	34.0	40.0	30.0	41.0
24	35.83012812215855	36.0	34.0	40.0	30.0	41.0
25	35.710400076365275	36.0	34.0	40.0	30.0	41.0
26	35.54847051038535	36.0	34.0	40.0	30.0	41.0
27	35.57228814299721	36.0	34.0	40.0	30.0	41.0
28	35.60480365064829	36.0	34.0	40.0	30.0	41.0
29	35.684113111205306	36.0	34.0	40.0	30.0	41.0
30	35.6581452784582	36.0	34.0	40.0	30.0	41.0
31	35.54679193052055	36.0	34.0	40.0	30.0	41.0
32	35.44089093356545	36.0	34.0	40.0	30.0	41.0
33	35.29022726757938	36.0	34.0	40.0	29.0	41.0
34	35.23209865357671	36.0	34.0	40.0	29.0	41.0
35	35.128915338094295	35.0	34.0	40.0	29.0	41.0
36	34.93520681721843	35.0	34.0	40.0	27.0	41.0
37	34.853650082027954	35.0	33.0	40.0	27.0	41.0
38	34.85173609650499	35.0	33.0	40.0	27.0	41.0
39	34.811259266569806	35.0	34.0	40.0	27.0	41.0
40	34.68226748230817	35.0	33.0	40.0	27.0	41.0
41	34.67256609964374	35.0	34.0	40.0	27.0	41.0
42	34.63134543636605	35.0	33.0	40.0	27.0	41.0
43	34.56684469051032	35.0	33.0	40.0	27.0	41.0
44	34.486738410113865	35.0	33.0	40.0	27.0	41.0
45	34.39932848715866	35.0	33.0	39.0	27.0	41.0
46	34.34131393569138	35.0	33.0	39.0	27.0	41.0
47	34.29391731194243	35.0	33.0	39.0	27.0	41.0
48	34.210991421850174	35.0	33.0	39.0	26.0	41.0
49	34.185195491860306	35.0	33.0	39.0	26.0	41.0
50	34.02328210496342	35.0	33.0	39.0	26.0	41.0
51	33.942328849570124	35.0	33.0	39.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	6.0
14	13.0
15	44.0
16	160.0
17	366.0
18	1007.0
19	1879.0
20	3535.0
21	5916.0
22	9050.0
23	13433.0
24	18885.0
25	25707.0
26	34080.0
27	42507.0
28	51131.0
29	62259.0
30	77171.0
31	97416.0
32	125164.0
33	183682.0
34	277649.0
35	383947.0
36	178967.0
37	223365.0
38	290876.0
39	363840.0
40	273.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.85879867072654	25.267318899434056	30.699365132781736	12.17451729705767
2	27.59132283418705	26.78920434505454	31.69272038337955	13.926752437378859
3	29.12922557201148	26.123475525901096	31.012187703249733	13.735111198837693
4	21.786915004805188	39.16070197805469	25.9840522778531	13.06833073928702
5	23.129495762698156	34.2865914231445	28.618613711449292	13.965299102708054
6	21.41212654631586	40.97093913105381	26.165379350959906	11.451554971670426
7	90.46247099899367	4.264240019932631	3.692754359453924	1.5805346216197849
8	92.56437657139344	2.482922977857307	3.1937509909688355	1.7589494597804174
9	89.08356819968871	4.453454396018651	4.363175112687314	2.099802291605321
10	58.50324067033177	24.143155762504005	8.607797994440867	8.74580557272336
11	51.06478590219421	18.8304707142418	17.07694124727787	13.027802136286123
12	47.45409994143172	20.562562896185295	18.57783433266136	13.405502829721621
13	21.934913166861357	42.429119437226774	18.8358502593507	16.80011713656117
14	14.622048530777471	44.56010691138069	23.50258541746888	17.31525914037296
15	14.10925249400565	23.933151264718923	47.36576214806449	14.591834093210934
16	15.878152089852154	18.018482984458373	47.14443229215541	18.95893263353406
17	16.786445811397193	19.33266945162616	25.536174811756368	38.34470992522028
18	22.955489724664364	24.505526774764512	30.82790794748917	21.711075553081955
19	32.25858381250384	25.730121569629922	21.23896991014137	20.772324707724866
20	36.23216660572545	23.308031943981543	21.63572147384975	18.824079976443254
21	24.79205833530179	29.38465284541533	23.85435913034193	21.96892968894095
22	23.494172294291047	26.303791406318254	22.204497137920214	27.997539161470485
23	22.275199730780056	31.865432094770597	21.787238586465875	24.072129587983472
24	22.759075656628085	22.54191191460033	36.73258564397604	17.966426784795544
25	18.915370452464238	25.79370536595468	32.81142307978553	22.479501101795556
26	17.72410456864947	38.15331137292463	23.731357651573738	20.391226406852166
27	19.75688500878524	35.35813209250552	25.69206027679175	19.19292262191748
28	15.788277283596674	29.6474011538922	37.66239754595668	16.90192401655444
29	16.42921165800007	24.85305347834106	36.02361822541346	22.69411663824541
30	19.85339323908478	31.526803886862908	27.90119272200129	20.718610152051024
31	30.53830236117538	27.95777906491372	20.56122812183497	20.942690452075936
32	33.54672195598643	27.383421617196426	21.462524389967676	17.60733203684948
33	29.792891558078054	29.073245944712838	21.147841224950735	19.986021272258373
34	19.571149135551593	28.36371225824405	26.414577677395556	25.650560928808797
35	21.65416562850884	25.130443856963964	28.72806520817626	24.48732530635094
36	31.597951404506198	25.67466776252989	22.84227659113192	19.885104241831993
37	21.977868632317396	33.751144670124674	26.085980500969125	18.1850061965888
38	21.305263702874374	33.409725570393576	21.681548726544374	23.603462000187676
39	22.89554622202232	31.79735860290382	24.595199342482065	20.711895832591793
40	26.49430010904702	25.64328034144337	23.359198294077483	24.503221255432127
41	17.184532149455897	23.959725408602743	26.967416944677243	31.88832549726412
42	23.06858151507405	26.18167977711695	21.420580117201276	29.32915859060772
43	22.64917923511767	27.094827223572278	23.427029099698746	26.82896444161131
44	20.901191104092984	30.978980135321848	26.054754870712948	22.065073889872217
45	18.2892803867448	37.14741733297524	20.203791729899915	24.359510550380048
46	21.984785190314554	34.32643241511644	22.660423697826502	21.0283586967425
47	22.113692034390258	27.20593707631026	25.84859290514851	24.83177798415097
48	23.722014231121438	21.543419805139123	30.7796538323394	23.954912131400043
49	19.98974246135626	24.619710653279014	31.116340550282974	24.274206335081754
50	18.727409955313373	33.27657171702136	24.92318980329471	23.072828524370554
51	17.302679903313802	35.586661640364866	22.11086069485926	24.999797761462073
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	322.0
1	639.0
2	956.0
3	3276.5
4	5597.0
5	4172.0
6	2747.0
7	3049.0
8	3351.0
9	3621.5
10	3892.0
11	3974.0
12	4056.0
13	4068.0
14	4080.0
15	3887.5
16	3695.0
17	4041.5
18	4388.0
19	4197.0
20	4006.0
21	3918.0
22	3830.0
23	4822.5
24	5815.0
25	7930.5
26	13061.0
27	16076.0
28	21662.5
29	27249.0
30	30941.5
31	34634.0
32	40436.5
33	46239.0
34	54000.0
35	61761.0
36	62666.5
37	63572.0
38	82115.5
39	100659.0
40	143836.0
41	187013.0
42	223806.0
43	260599.0
44	261765.0
45	262931.0
46	242507.5
47	222084.0
48	207947.5
49	193811.0
50	177414.0
51	161017.0
52	152459.0
53	143901.0
54	128953.0
55	114005.0
56	107242.0
57	100479.0
58	102373.5
59	104268.0
60	104754.0
61	105240.0
62	90703.0
63	76166.0
64	61709.0
65	47252.0
66	39038.0
67	30824.0
68	26483.0
69	22142.0
70	17599.5
71	13057.0
72	10440.0
73	7823.0
74	6619.0
75	4376.5
76	3338.0
77	2793.5
78	2249.0
79	1544.0
80	839.0
81	705.0
82	571.0
83	385.0
84	199.0
85	143.0
86	87.0
87	59.5
88	32.0
89	21.0
90	10.0
91	7.0
92	4.0
93	2.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2472328.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.450645011160091
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.81467213541615	12.022868758241255
2	9.47579573316353	2.928143121427503
3	3.287960496325917	1.5240333131834845
4	1.6171466157830088	0.9994383316584867
5	0.903245687161406	0.6977864235096123
6	0.557160673464025	0.516509506792292
7	0.38125818118205024	0.412347937053109
8	0.2893642089149459	0.3576690936703997
9	0.21628690344505297	0.3007594949123315
>10	2.3613712450890647	8.870508349182646
>50	1.1605108893374885	13.147692823628368
>100	1.9152676158434423	55.7493601764983
>500	0.016537966609796204	1.5838414579800435
>1k	0.0034216482640957667	0.8890412122621701
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2975	0.1203319300675315	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2912	0.11778372448963083	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2548	0.10306075892842698	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05124724551111341	0.0
2	0.0	0.0	0.0	0.18286408599506215	0.0
3	0.0	0.0	0.0	0.2694626279360991	0.0
4	0.0	0.0	0.0	0.4782536944936109	0.0
5	4.044770758572487E-5	0.0	0.0	0.8098035535737976	0.0
6	4.044770758572487E-5	0.0	0.0	1.1498474312469866	0.0
7	4.044770758572487E-5	0.0	0.0	1.326967942764876	0.0
8	4.044770758572487E-5	0.0	0.0	1.7877482275814536	0.0
9	4.044770758572487E-5	0.0	0.0	1.9128934348516864	0.0
10	8.089541517144974E-5	0.0	0.0	2.1292482227277287	0.0
11	8.089541517144974E-5	0.0	0.0	2.464559718613388	0.0
12	8.089541517144974E-5	0.0	0.0	2.7298157849605715	0.0
13	8.089541517144974E-5	0.0	0.0	2.836638180694471	0.0
14	8.089541517144974E-5	0.0	0.0	2.876964545157439	0.0
15	8.089541517144974E-5	0.0	0.0	2.9380405836118832	0.0
16	8.089541517144974E-5	0.0	0.0	3.0813872592956923	0.0
17	8.089541517144974E-5	0.0	0.0	3.260206574532182	0.0
18	8.089541517144974E-5	0.0	0.0	3.4658022721904214	0.0
19	8.089541517144974E-5	0.0	0.0	3.592565387764083	0.0
20	1.2134312275717462E-4	0.0	0.0	3.7232114832659744	0.0
21	1.2134312275717462E-4	0.0	0.0	3.9062778077989653	0.0
22	1.2134312275717462E-4	0.0	0.0	4.101114415239402	0.0
23	1.2134312275717462E-4	0.0	0.0	4.323738597791231	0.0
24	1.2134312275717462E-4	0.0	0.0	4.4794622719962724	0.0
25	1.2134312275717462E-4	0.0	0.0	4.605780462786491	0.0
26	1.2134312275717462E-4	0.0	0.0	4.730157163612595	0.0
27	2.0223853792862434E-4	0.0	0.0	4.846808352289826	0.0
28	2.0223853792862434E-4	0.0	0.0	4.970537889794558	0.0
29	2.4268624551434923E-4	0.0	0.0	5.098676227426134	0.0
30	2.4268624551434923E-4	0.0	0.0	5.265644364340007	0.0
31	2.4268624551434923E-4	0.0	0.0	5.443331143764096	0.0
32	2.831339531000741E-4	0.0	0.0	5.593311243491964	0.0
33	2.831339531000741E-4	0.0	0.0	5.736819710006116	0.0
34	3.2358166068579896E-4	0.0	0.0	5.900228448652444	0.0
35	3.2358166068579896E-4	0.0	0.0	6.1120530932788855	0.0
36	3.2358166068579896E-4	0.0	0.0	6.300175381260091	0.0
37	3.2358166068579896E-4	0.0	0.0	6.484616927850997	0.0
38	3.2358166068579896E-4	0.0	0.0	6.650209842706955	0.0
39	3.2358166068579896E-4	0.0	0.0	6.828705576282759	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGATCG	20	7.034863E-4	45.000004	16
CGTGCGC	20	7.034863E-4	45.000004	27
CGACACT	20	7.034863E-4	45.000004	40
ACTTACG	40	6.8212103E-9	45.000004	38
CTACGTT	20	7.034863E-4	45.000004	30
GCGTGTT	20	7.034863E-4	45.000004	36
ATACGTC	40	6.8212103E-9	45.000004	20
ATCGGCC	40	6.8212103E-9	45.000004	30
CTTACGA	20	7.034863E-4	45.000004	31
CGAACGC	25	3.8921142E-5	45.0	24
GACACGC	35	1.2126839E-7	45.0	33
CGACATA	25	3.8921142E-5	45.0	23
TCTAGCG	45	3.8562575E-10	45.0	1
TAGCCGT	35	1.2126839E-7	45.0	44
ATATACG	50	2.1827873E-11	45.0	1
TATTACG	100	0.0	45.0	1
CGAATCC	45	3.8562575E-10	45.0	28
TCATCGG	35	1.2126839E-7	45.0	2
CCGCGAA	25	3.8921142E-5	45.0	33
CTATGCG	130	0.0	44.999996	1
>>END_MODULE