FastQCFastQC Report
Thu 26 May 2016
SRR1545269_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545269_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2239293
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30010.13401551293198344No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA207.0347195E-445.00000428
CGTAGAC207.0347195E-445.00000414
AATCGGC207.0347195E-445.00000427
ACGCATT253.8919956E-545.011
GTACTCG253.8919956E-545.01
ATACGAT453.8562575E-1045.011
TACGTCC253.8919956E-545.019
TAGACCG650.041.538461
CGTATTT4300.041.3372081
CGTTAGA556.184564E-1140.9090926
TACGGGA4150.040.662654
CCCGTTA501.0822987E-940.519
TATTACG501.0822987E-940.51
CACGTTA451.9297659E-840.024
AATACGG1650.039.5454522
CGCGATC403.4601726E-739.37500433
ATAGGGC9550.038.8743484
TCGATAG1100.038.8636321
TCAATCG356.2509444E-638.57142615
AACGCAT356.2509444E-638.57142629
Produced by FastQC (version 0.11.5)