##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545269_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2239293
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.199496448209324	31.0	31.0	33.0	30.0	34.0
2	31.528603001036487	31.0	31.0	34.0	30.0	34.0
3	31.642756441430397	31.0	31.0	34.0	30.0	34.0
4	30.318812678823182	35.0	33.0	37.0	0.0	37.0
5	32.85183850438509	35.0	33.0	37.0	19.0	37.0
6	34.455961770076534	35.0	33.0	37.0	30.0	37.0
7	35.44014249140242	36.0	35.0	37.0	33.0	37.0
8	35.612938994584454	37.0	35.0	37.0	35.0	37.0
9	37.61174397454911	39.0	37.0	39.0	35.0	39.0
10	37.044599791094775	39.0	37.0	39.0	33.0	39.0
11	36.60872695087244	39.0	35.0	39.0	32.0	39.0
12	35.68416058104054	37.0	35.0	39.0	31.0	39.0
13	35.29027420708233	37.0	35.0	39.0	30.0	39.0
14	36.06635397868881	38.0	35.0	40.0	30.0	41.0
15	36.357174340294016	38.0	35.0	40.0	31.0	41.0
16	36.521138591510805	38.0	35.0	40.0	31.0	41.0
17	36.44144915381774	38.0	35.0	40.0	31.0	41.0
18	36.41141199476799	38.0	35.0	40.0	31.0	41.0
19	36.405010420699746	38.0	35.0	40.0	31.0	41.0
20	36.31249684610277	38.0	35.0	40.0	31.0	41.0
21	36.187985225694	38.0	34.0	40.0	30.0	41.0
22	36.091424838107386	38.0	34.0	40.0	30.0	41.0
23	36.02793470974991	37.0	34.0	40.0	30.0	41.0
24	35.958786545574874	37.0	34.0	40.0	30.0	41.0
25	35.838494560559965	37.0	34.0	40.0	30.0	41.0
26	35.69482466117654	37.0	34.0	40.0	30.0	41.0
27	35.7062313864242	37.0	34.0	40.0	30.0	41.0
28	35.69931491769947	37.0	34.0	40.0	30.0	41.0
29	35.68966722979083	37.0	34.0	40.0	30.0	41.0
30	35.614793597800734	37.0	34.0	40.0	29.0	41.0
31	35.49620304265677	37.0	34.0	40.0	29.0	41.0
32	35.426123334463156	37.0	34.0	40.0	29.0	41.0
33	35.280837746556614	36.0	34.0	40.0	29.0	41.0
34	35.11994857305408	36.0	34.0	40.0	27.0	41.0
35	35.00251686581434	36.0	34.0	40.0	27.0	41.0
36	34.84936138325802	36.0	33.0	40.0	26.0	41.0
37	34.819486328944	36.0	33.0	40.0	27.0	41.0
38	34.772003485028534	36.0	33.0	40.0	27.0	41.0
39	34.67849763295826	36.0	33.0	40.0	26.0	41.0
40	34.55221893695912	36.0	33.0	40.0	25.0	41.0
41	34.49137830556341	35.0	33.0	40.0	25.0	41.0
42	34.441394672336315	35.0	33.0	40.0	26.0	41.0
43	34.37296101939317	35.0	33.0	40.0	26.0	41.0
44	34.29738091442254	35.0	33.0	40.0	26.0	41.0
45	34.20051551985381	35.0	33.0	39.0	25.0	41.0
46	34.136158600058145	35.0	33.0	39.0	25.0	41.0
47	34.05330209132972	35.0	33.0	39.0	25.0	41.0
48	33.928787344934314	35.0	33.0	39.0	24.0	41.0
49	33.86419061730644	35.0	33.0	39.0	24.0	41.0
50	33.73207615082082	35.0	33.0	39.0	24.0	41.0
51	33.64216473681649	35.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	4.0
12	8.0
13	13.0
14	31.0
15	87.0
16	219.0
17	502.0
18	1204.0
19	2324.0
20	4054.0
21	6504.0
22	9754.0
23	14028.0
24	19332.0
25	25808.0
26	33300.0
27	40808.0
28	49121.0
29	59237.0
30	72542.0
31	89679.0
32	113352.0
33	161433.0
34	234120.0
35	289489.0
36	178216.0
37	225785.0
38	283172.0
39	325005.0
40	160.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.03381603032743	24.621878423234474	30.286032243212478	13.058273303225617
2	28.71933239643048	26.321209417436663	30.993755618402773	13.965702567730082
3	29.41999997320583	26.269452010076392	30.482746116743098	13.827801899974679
4	23.02811646354452	38.38273955217115	25.81180756604875	12.777336418235578
5	24.19803929186578	32.48118937539661	28.54941269409586	14.771358638641749
6	22.97564454495236	40.722987121381614	25.39118373522357	10.910184598442454
7	89.52227332466094	4.060031447425594	4.51687206631736	1.900823161596093
8	91.57586791902624	2.748680052141457	3.732249419794551	1.9432026090377634
9	87.5482574187478	4.19221602532585	5.364059102582824	2.895467453343533
10	56.77483920148011	21.86837542027774	11.106139303789186	10.250646074452964
11	46.851528585138254	21.255235469409318	17.99215198725669	13.901083958195734
12	43.11155351264886	20.734312124407122	20.417337079158465	15.736797283785553
13	24.522338077241344	34.79236526885941	21.947686167017892	18.73761048688135
14	18.499499618852916	35.08598472821556	26.554363363793843	19.860152289137687
15	18.042748313865133	23.151235680190133	41.11561104330697	17.690404962637764
16	21.79728155270436	19.22222772991297	38.84623405690993	20.134256660472747
17	22.381126543065154	20.88538659300056	25.62648121527643	31.10700564865786
18	25.89299390477262	24.140565794650364	28.927969676143316	21.038470624433696
19	32.09834532595779	25.557530881398726	20.69416552456512	21.64995826807836
20	33.0758413481398	24.950821531617347	22.55502071412718	19.41831640611568
21	26.70709013961103	27.38980562168506	25.24077912091004	20.662325117793877
22	26.3216559869566	24.397477239467992	24.83931312249	24.44155365108541
23	25.15874430009829	28.164023198393423	24.16919090087809	22.5080416006302
24	24.21210623174368	23.033430640831725	32.9927794174322	19.761683709992393
25	22.38237693772097	25.373276297474245	28.874738589367272	23.369608175437513
26	20.95375638650235	32.666515726168925	24.01387402184529	22.365853865483437
27	21.406175967146773	30.113567094614236	26.891389380487503	21.588867557751488
28	19.039536139308254	28.09962787361904	32.41652610891027	20.444309878162436
29	20.6240094529836	24.696410876111344	31.952495720747574	22.727083950157486
30	23.247471411735756	27.63314135309671	27.50810188751539	21.61128534765214
31	29.610461873457382	25.632465246843534	22.774331005366427	21.982741874332657
32	32.837998421823315	24.701412454734598	22.29158935431853	20.16899976912356
33	30.093918035737172	25.72267228987006	23.31521600790964	20.868193666483126
34	22.181286682895003	25.042278969299687	27.859507442750903	24.916926905054407
35	23.594857841291873	25.111809843553303	28.279461419296183	23.01387089585865
36	29.177780665594007	26.241407444224585	23.63723728873354	20.943574601447867
37	23.931437288465602	30.30809277749718	25.906882216842547	19.853587717194667
38	23.834710330448047	29.757338588563442	23.383943057027373	23.024008023961134
39	23.741332643829992	29.423661843269283	24.08469994770671	22.750305565194015
40	26.27771354619516	24.126498854772464	24.50809250955547	25.087695089476902
41	19.62722162754048	23.607406444802	26.5030971829055	30.262274744752027
42	22.704219590736898	24.809973505030385	24.161643875991217	28.324163028241507
43	22.682025085596212	26.480500765196872	24.617144786323184	26.22032936288373
44	23.091440021471062	27.455942567587183	25.35376120945316	24.098856201488594
45	20.327174693083933	31.447961477126935	22.263187532850772	25.961676296938364
46	22.64000289377049	30.09119396166558	24.293515855227522	22.97528728933641
47	22.60030286344842	24.410383098594064	28.008750976312612	24.980563061644904
48	23.338616250754143	22.34776779992614	30.13736925002668	24.176246699293035
49	22.76995462407108	23.111044423396134	29.695042140532745	24.423958812000038
50	20.604092452394575	29.08194684661632	25.85418701349042	24.459773687498686
51	19.243484439061792	30.487256468894426	24.34223658985224	25.927022502191537
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	472.0
1	908.0
2	1344.0
3	3921.0
4	6498.0
5	4834.5
6	3171.0
7	3230.5
8	3290.0
9	3427.5
10	3565.0
11	3723.5
12	3882.0
13	3793.0
14	3704.0
15	3708.0
16	3712.0
17	3570.5
18	3429.0
19	3450.5
20	3472.0
21	4186.0
22	4900.0
23	5564.5
24	6229.0
25	8095.5
26	12113.5
27	14265.0
28	18692.5
29	23120.0
30	25414.5
31	27709.0
32	33185.0
33	38661.0
34	46411.5
35	54162.0
36	58871.0
37	63580.0
38	71793.0
39	80006.0
40	107812.5
41	135619.0
42	157389.5
43	179160.0
44	180232.5
45	181305.0
46	172302.5
47	163300.0
48	158039.5
49	152779.0
50	152657.5
51	152536.0
52	141398.0
53	130260.0
54	127424.5
55	124589.0
56	121889.5
57	119190.0
58	119639.0
59	120088.0
60	117987.5
61	115887.0
62	104923.5
63	93960.0
64	81322.0
65	68684.0
66	58742.5
67	48801.0
68	40440.0
69	32079.0
70	28155.0
71	24231.0
72	20187.0
73	16143.0
74	12665.0
75	7652.0
76	6117.0
77	4743.0
78	3369.0
79	2516.0
80	1663.0
81	1283.5
82	904.0
83	546.0
84	188.0
85	130.5
86	73.0
87	51.5
88	30.0
89	19.0
90	8.0
91	6.5
92	5.0
93	3.5
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2239293.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.298446103243514
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.89263739153506	14.436126732517382
2	7.86818681896842	2.8795118487428932
3	2.724536055566753	1.4956432850739574
4	1.4472448435717205	1.0592932707317686
5	0.8762184240844656	0.801671780388928
6	0.5884839145105475	0.646100471537821
7	0.443375708348157	0.5679160551887317
8	0.29690399390730193	0.43463053842804034
9	0.25152287250394284	0.41422299536217494
>10	3.4992680751469134	16.17851982122028
>50	1.6398859030903343	21.905335624315143
>100	1.4677453913957748	38.485274780949105
>500	0.002926445405100852	0.3297182818512471
>1k	0.0010641619654912188	0.3660345136925331
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3001	0.13401551293198344	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03393928351493083	0.0
2	0.0	0.0	0.0	0.12320853054959757	0.0
3	0.0	0.0	0.0	0.18090531252498	0.0
4	0.0	0.0	0.0	0.3243434423275561	0.0
5	0.0	0.0	0.0	0.583577048648837	0.0
6	0.0	0.0	0.0	0.8590211285436966	0.0
7	0.0	0.0	0.0	1.0190716444877914	0.0
8	0.0	0.0	0.0	1.394234698183757	0.0
9	0.0	0.0	0.0	1.5117271388782085	0.0
10	0.0	0.0	0.0	1.7093788084006871	0.0
11	0.0	0.0	0.0	1.9989791420774325	0.0
12	0.0	0.0	0.0	2.2444137502327743	0.0
13	0.0	0.0	0.0	2.337166239522921	0.0
14	0.0	0.0	0.0	2.374678079197318	0.0
15	0.0	0.0	0.0	2.4363939868521003	0.0
16	0.0	0.0	0.0	2.569248419032257	0.0
17	0.0	0.0	0.0	2.7102304164752	0.0
18	0.0	0.0	0.0	2.864654156468135	0.0
19	0.0	0.0	0.0	2.9733491776199004	0.0
20	0.0	0.0	0.0	3.085795382739106	0.0
21	0.0	0.0	0.0	3.244550847075394	0.0
22	0.0	0.0	0.0	3.4108979932505483	0.0
23	0.0	0.0	0.0	3.5773344533296894	0.0
24	0.0	0.0	0.0	3.7127789887254594	0.0
25	0.0	0.0	0.0	3.832772218731537	0.0
26	0.0	0.0	0.0	3.9387878227637025	0.0
27	0.0	0.0	0.0	4.063827288345027	0.0
28	4.465695199333004E-5	0.0	0.0	4.182123554175358	0.0
29	4.465695199333004E-5	0.0	0.0	4.313906219507675	0.0
30	4.465695199333004E-5	0.0	0.0	4.481146504722696	0.0
31	8.931390398666008E-5	0.0	0.0	4.6345431348197845	0.0
32	1.3397085597999012E-4	0.0	0.0	4.7675315378559215	0.0
33	1.3397085597999012E-4	0.0	0.0	4.909272703482751	0.0
34	1.3397085597999012E-4	0.0	0.0	5.0655720354594065	0.0
35	1.3397085597999012E-4	0.0	0.0	5.256659133038865	0.0
36	1.3397085597999012E-4	0.0	0.0	5.4133603775834604	0.0
37	1.3397085597999012E-4	0.0	0.0	5.578948355574728	0.0
38	1.3397085597999012E-4	0.0	0.0	5.799151785853839	0.0
39	1.7862780797332016E-4	0.0	0.0	6.205931961561082	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGAAA	20	7.0347195E-4	45.000004	28
CGTAGAC	20	7.0347195E-4	45.000004	14
AATCGGC	20	7.0347195E-4	45.000004	27
ACGCATT	25	3.8919956E-5	45.0	11
GTACTCG	25	3.8919956E-5	45.0	1
ATACGAT	45	3.8562575E-10	45.0	11
TACGTCC	25	3.8919956E-5	45.0	19
TAGACCG	65	0.0	41.53846	1
CGTATTT	430	0.0	41.337208	1
CGTTAGA	55	6.184564E-11	40.90909	26
TACGGGA	415	0.0	40.66265	4
CCCGTTA	50	1.0822987E-9	40.5	19
TATTACG	50	1.0822987E-9	40.5	1
CACGTTA	45	1.9297659E-8	40.0	24
AATACGG	165	0.0	39.545452	2
CGCGATC	40	3.4601726E-7	39.375004	33
ATAGGGC	955	0.0	38.874348	4
TCGATAG	110	0.0	38.863632	1
TCAATCG	35	6.2509444E-6	38.571426	15
AACGCAT	35	6.2509444E-6	38.571426	29
>>END_MODULE