FastQCFastQC Report
Thu 26 May 2016
SRR1545268_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545268_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2227588
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22860.10262220841555979No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG600.045.0000041
TAGACCG302.1664218E-645.0000041
GTTAGTC551.8189894E-1245.00000422
GTCTAGA600.045.00000431
CCGTAGA302.1664218E-645.00000428
TACGATG302.1664218E-645.0000041
GGTATAC600.045.0000048
GAACGAT600.045.00000443
ACGTCCA302.1664218E-645.00000414
CGGCTAG302.1664218E-645.00000420
ATACCGG1150.045.0000042
TACGTTC302.1664218E-645.00000431
GTACGAC302.1664218E-645.00000435
ACGGTAC302.1664218E-645.00000413
TCGTTTG700.045.01
AGATCGA207.034714E-445.024
CGTATTA207.034714E-445.031
GCGCGTT207.034714E-445.018
CGTGCAT406.8193913E-945.044
GACGTAT207.034714E-445.021
Produced by FastQC (version 0.11.5)