##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545268_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2227588
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.233635214411283	31.0	31.0	33.0	30.0	34.0
2	31.545784049833273	31.0	31.0	34.0	30.0	34.0
3	31.653962043250367	31.0	31.0	34.0	30.0	34.0
4	30.287857539185882	35.0	33.0	37.0	0.0	37.0
5	32.8293504005229	35.0	33.0	37.0	19.0	37.0
6	34.44374453444713	35.0	33.0	37.0	30.0	37.0
7	35.46872177440353	36.0	35.0	37.0	33.0	37.0
8	35.645222545641296	37.0	35.0	37.0	35.0	37.0
9	37.68813039035944	39.0	37.0	39.0	35.0	39.0
10	37.14287426579781	39.0	37.0	39.0	33.0	39.0
11	36.64808393652686	39.0	35.0	39.0	32.0	39.0
12	35.211601516977105	35.0	35.0	39.0	31.0	39.0
13	34.629571087651755	35.0	33.0	39.0	27.0	39.0
14	35.372417610437836	36.0	33.0	40.0	27.0	41.0
15	35.79758285643485	36.0	34.0	40.0	30.0	41.0
16	36.067758490349206	36.0	34.0	40.0	31.0	41.0
17	36.02395371136853	36.0	34.0	40.0	31.0	41.0
18	36.01664311353805	36.0	35.0	40.0	31.0	41.0
19	35.97571049942808	36.0	35.0	40.0	31.0	41.0
20	35.84330091560917	36.0	34.0	40.0	31.0	41.0
21	35.62910511279465	35.0	34.0	40.0	30.0	41.0
22	35.484189176813665	35.0	34.0	40.0	30.0	41.0
23	35.47438170792804	35.0	34.0	40.0	30.0	41.0
24	35.389831512829126	35.0	34.0	40.0	30.0	41.0
25	35.271994192822014	35.0	34.0	40.0	30.0	41.0
26	35.061448077472136	35.0	34.0	40.0	29.0	41.0
27	35.039638389145566	35.0	34.0	40.0	29.0	41.0
28	35.10115111052852	35.0	34.0	40.0	29.0	41.0
29	35.21325352803122	36.0	34.0	40.0	29.0	41.0
30	35.20046435875934	36.0	34.0	40.0	29.0	41.0
31	35.03583023431622	35.0	34.0	40.0	29.0	41.0
32	34.902240899124976	35.0	34.0	40.0	29.0	41.0
33	34.775506063060135	35.0	34.0	40.0	29.0	41.0
34	34.734594996920436	35.0	34.0	40.0	28.0	41.0
35	34.584182981772216	35.0	33.0	40.0	27.0	41.0
36	34.34774473556151	35.0	33.0	39.0	26.0	41.0
37	34.25900660265722	35.0	33.0	39.0	26.0	41.0
38	34.275360614260805	35.0	33.0	39.0	27.0	41.0
39	34.235570491491245	35.0	33.0	39.0	26.0	41.0
40	34.118607659944296	35.0	33.0	39.0	26.0	41.0
41	34.11361526458214	35.0	33.0	39.0	26.0	41.0
42	34.072298378335674	35.0	33.0	39.0	26.0	41.0
43	33.97743703054604	35.0	33.0	39.0	26.0	41.0
44	33.90937013487234	35.0	33.0	39.0	26.0	41.0
45	33.82622504700151	35.0	33.0	38.0	26.0	41.0
46	33.771669177603755	35.0	33.0	38.0	26.0	40.0
47	33.69075340682388	35.0	33.0	38.0	25.0	40.0
48	33.63378955174835	35.0	33.0	38.0	25.0	40.0
49	33.62678062550167	35.0	33.0	38.0	24.0	40.0
50	33.44534985823231	35.0	33.0	38.0	24.0	40.0
51	33.3302774121606	35.0	32.0	38.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	3.0
12	2.0
13	6.0
14	29.0
15	52.0
16	162.0
17	485.0
18	1135.0
19	2321.0
20	4042.0
21	6533.0
22	10059.0
23	14483.0
24	20228.0
25	27074.0
26	35014.0
27	43104.0
28	50940.0
29	61736.0
30	75813.0
31	94510.0
32	121684.0
33	181263.0
34	277367.0
35	384489.0
36	148632.0
37	178757.0
38	223743.0
39	263775.0
40	146.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.24434320888782	25.204211909922304	28.922718204623116	12.628726676566762
2	27.93128711413421	27.98264310994672	29.908807194149006	14.177262581770059
3	28.83607740749187	26.628398070020133	30.09016927726312	14.445355245224881
4	21.83294217781744	38.26591811412165	26.22168013115531	13.679459576905604
5	23.480778312686187	32.84925219564839	29.52462484085926	14.145344650806162
6	21.906833759205025	41.74308714178744	25.71835545890892	10.631723640098619
7	89.70756710845991	4.390084701479807	4.2491699542285195	1.6531782358317606
8	91.52361208625652	2.839708240482531	3.414545239065752	2.2221344341951923
9	89.19530900687201	3.8275031109882076	4.726412604126077	2.250775278013708
10	64.79200821695933	18.549345749752646	8.028683939759059	8.629962093528965
11	58.85397120113773	17.306566564373664	13.056992585702561	10.78246964878604
12	51.593651967958166	20.722997250838127	15.782721041772534	11.900629739431169
13	21.01340104184436	47.67035017247355	17.133823669367946	14.182425116314148
14	13.603188740467267	48.895666523612086	22.95958678175677	14.541557954163874
15	12.273050492281337	22.359071785267293	53.077499070743784	12.290378651707586
16	14.455814989127253	15.94904443730169	51.23088291012521	18.364257663445844
17	15.327744627821662	17.640694778388106	24.643560658434144	42.38799993535609
18	22.448002054239833	24.04470665131972	32.41932529713753	21.087965997302913
19	34.50979265465607	23.95272375322546	19.818027391061545	21.71945620105693
20	38.67708032185485	22.534238826928497	20.096085990766696	18.692594860449958
21	24.312529965146158	29.875138490600595	23.571145113010125	22.241186431243122
22	24.878568209202058	25.378436227884148	20.87333923508297	28.869656327830818
23	21.994372388430893	32.49869365430232	20.288266950621033	25.218667006645752
24	22.87501099844316	21.914465332009332	37.88537198081512	17.325151688732387
25	18.512085717825737	24.32702995347434	34.88010350208387	22.280780826616052
26	17.781160609592078	39.29824545652069	22.4663627205749	20.454231213312333
27	19.49588523551034	36.48578642011	26.356534511767887	17.661793832611774
28	15.235537271703745	29.362431472965376	39.55933502963744	15.84269622569344
29	15.469557207167572	24.4614354180396	37.904675370849546	22.16433200394328
30	19.79091286180389	31.18727520528931	27.81614014799864	21.205671784908162
31	33.16717454035486	27.039515386148604	19.112914955548334	20.6803951179482
32	35.01024426419966	26.63127113272293	21.634341718486542	16.72414288459087
33	30.63326791130137	28.674243172435837	20.264474400113485	20.428014516149307
34	20.05020677073139	28.012361352278788	25.0261718055583	26.91126007143152
35	20.22631653609195	24.897063550351323	29.626753241622776	25.249866671933948
36	33.28959394645688	24.378655298915238	23.098840539633002	19.232910214994874
37	21.77112643810256	33.10217149670406	26.94008048166896	18.186621583524424
38	20.933000177770754	34.717146976909554	19.83266205420392	24.517190791115773
39	20.93362866023699	33.13610954988086	25.2495075390961	20.680754250786052
40	26.201838041864118	24.630003393805318	22.746037418050374	26.42212114628019
41	16.577122879096134	22.955007838074188	26.341361149368737	34.126508133460945
42	22.754073015297262	26.016301039509997	21.20495351923246	30.024672425960276
43	23.068269356811044	27.343970249435714	22.252095091192807	27.335665302560436
44	20.246383083406805	31.32904289303049	26.509031293039826	21.91554273052288
45	17.960727028516942	39.08927503649687	18.652596440634444	24.29740149435174
46	22.83398007171883	34.30593987757162	22.08060916111956	20.779470889589994
47	22.624874976880825	27.002300245826426	24.470503522195308	25.902321255097444
48	24.828110045484173	21.025656449935983	29.80429953833474	24.341933966245104
49	20.040375509295256	22.572217124531107	32.011485068154435	25.375922298019205
50	18.401113670930172	34.885625169465804	23.810013341784927	22.903247817819093
51	16.717498927090645	37.081677581312164	20.692650526039824	25.508172965557364
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	452.0
1	824.5
2	1197.0
3	2966.5
4	4736.0
5	3537.5
6	2339.0
7	2682.0
8	3025.0
9	3561.0
10	4097.0
11	4243.0
12	4389.0
13	4343.5
14	4298.0
15	4560.0
16	4822.0
17	4778.5
18	4735.0
19	4725.5
20	4716.0
21	4890.0
22	5064.0
23	5032.5
24	5001.0
25	5537.5
26	9737.0
27	13400.0
28	15454.5
29	17509.0
30	20721.5
31	23934.0
32	27729.5
33	31525.0
34	32839.5
35	34154.0
36	38249.0
37	42344.0
38	60941.0
39	79538.0
40	129501.5
41	179465.0
42	220975.5
43	262486.0
44	255123.5
45	247761.0
46	227074.0
47	206387.0
48	184683.5
49	162980.0
50	156325.5
51	149671.0
52	139294.5
53	128918.0
54	116742.5
55	104567.0
56	100458.5
57	96350.0
58	97414.0
59	98478.0
60	93501.5
61	88525.0
62	78984.0
63	69443.0
64	59380.5
65	49318.0
66	40369.0
67	31420.0
68	25148.0
69	18876.0
70	16515.0
71	14154.0
72	11785.5
73	9417.0
74	7274.0
75	4312.0
76	3493.0
77	2734.0
78	1975.0
79	1459.0
80	943.0
81	668.0
82	393.0
83	222.0
84	51.0
85	32.5
86	14.0
87	11.5
88	9.0
89	8.0
90	7.0
91	4.5
92	2.0
93	1.5
94	1.0
95	2.0
96	3.0
97	1.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2227588.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.236220756034916
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.10373384371218	12.052419514694291
2	9.132732953646554	2.782966707753474
3	3.218545779923194	1.4711542204894303
4	1.4955020226130291	0.9114319583051533
5	0.8675082077159342	0.6608773280216083
6	0.5406169154371161	0.4942175200827937
7	0.3498418495151007	0.37311873542381346
8	0.2389500660687085	0.29125567650335765
9	0.19574268938738823	0.2684140944197598
>10	1.8044963824710376	6.620535821389543
>50	0.9699140647435198	10.891212655632268
>100	2.0548590300701863	60.072706260188454
>500	0.024351986010498726	2.3875436102882808
>1k	0.003204208685591938	0.7221458968077752
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2286	0.10262220841555979	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06630490018800604	0.0
2	0.0	0.0	0.0	0.25677997906255556	0.0
3	0.0	0.0	0.0	0.36357710671811844	0.0
4	0.0	0.0	0.0	0.6157781420980899	0.0
5	0.0	0.0	0.0	0.9962793837998768	0.0
6	0.0	0.0	0.0	1.399720235519315	0.0
7	0.0	0.0	0.0	1.5895668319276275	0.0
8	4.4891604731216005E-5	0.0	0.0	2.0449023787163516	0.0
9	4.4891604731216005E-5	0.0	0.0	2.1617103342269757	0.0
10	4.4891604731216005E-5	0.0	0.0	2.3670445342675577	0.0
11	4.4891604731216005E-5	0.0	0.0	2.6767068237034857	0.0
12	4.4891604731216005E-5	0.0	0.0	2.911893940890326	0.0
13	8.978320946243201E-5	0.0	0.0	3.013528534001799	0.0
14	8.978320946243201E-5	0.0	0.0	3.0570284989863477	0.0
15	8.978320946243201E-5	0.0	0.0	3.127777668042744	0.0
16	8.978320946243201E-5	0.0	0.0	3.271116561949517	0.0
17	8.978320946243201E-5	0.0	0.0	3.436587016988779	0.0
18	8.978320946243201E-5	0.0	0.0	3.6234258758801	0.0
19	8.978320946243201E-5	0.0	0.0	3.7353855380797527	0.0
20	1.34674814193648E-4	0.0	0.0	3.844606812390801	0.0
21	1.34674814193648E-4	0.0	0.0	4.0029395022778	0.0
22	2.2445802365608002E-4	0.0	0.0	4.158892937114045	0.0
23	2.2445802365608002E-4	0.0	0.0	4.339357188133532	0.0
24	2.2445802365608002E-4	0.0	0.0	4.47030599913449	0.0
25	2.2445802365608002E-4	0.0	0.0	4.572254833479081	0.0
26	2.2445802365608002E-4	0.0	0.0	4.675909548803459	0.0
27	2.2445802365608002E-4	0.0	0.0	4.768924953806539	0.0
28	2.2445802365608002E-4	0.0	0.0	4.87235521110726	0.0
29	2.2445802365608002E-4	0.0	0.0	4.978523856296587	0.0
30	2.2445802365608002E-4	0.0	0.0	5.123254389950026	0.0
31	2.2445802365608002E-4	0.0	0.0	5.269017430512285	0.0
32	2.2445802365608002E-4	0.0	0.0	5.392648909942054	0.0
33	2.2445802365608002E-4	0.0	0.0	5.519063668865158	0.0
34	2.2445802365608002E-4	0.0	0.0	5.651897927264827	0.0
35	2.2445802365608002E-4	0.0	0.0	5.823742990175921	0.0
36	2.69349628387296E-4	0.0	0.0	5.974937914910656	0.0
37	3.1424123311851206E-4	0.0	0.0	6.124382067060875	0.0
38	3.1424123311851206E-4	0.0	0.0	6.261840160747858	0.0
39	3.1424123311851206E-4	0.0	0.0	6.399792062086885	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	60	0.0	45.000004	1
TAGACCG	30	2.1664218E-6	45.000004	1
GTTAGTC	55	1.8189894E-12	45.000004	22
GTCTAGA	60	0.0	45.000004	31
CCGTAGA	30	2.1664218E-6	45.000004	28
TACGATG	30	2.1664218E-6	45.000004	1
GGTATAC	60	0.0	45.000004	8
GAACGAT	60	0.0	45.000004	43
ACGTCCA	30	2.1664218E-6	45.000004	14
CGGCTAG	30	2.1664218E-6	45.000004	20
ATACCGG	115	0.0	45.000004	2
TACGTTC	30	2.1664218E-6	45.000004	31
GTACGAC	30	2.1664218E-6	45.000004	35
ACGGTAC	30	2.1664218E-6	45.000004	13
TCGTTTG	70	0.0	45.0	1
AGATCGA	20	7.034714E-4	45.0	24
CGTATTA	20	7.034714E-4	45.0	31
GCGCGTT	20	7.034714E-4	45.0	18
CGTGCAT	40	6.8193913E-9	45.0	44
GACGTAT	20	7.034714E-4	45.0	21
>>END_MODULE