FastQCFastQC Report
Thu 26 May 2016
SRR1545267_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545267_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3579702
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39700.11090308634629363No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTCAT302.166793E-645.00000426
CGTCGGT302.166793E-645.00000421
GACCGTT351.212884E-745.026
TCTACGA551.8189894E-1245.015
GTTCGAC207.0352876E-445.042
TACGAGT253.892465E-545.017
CTACGAC351.212884E-745.023
CACTACG207.0352876E-445.038
TACGACA253.892465E-545.033
TCGCTCA253.892465E-545.019
ACTACGT351.212884E-745.021
GATATCG253.892465E-545.01
CGGTCAT253.892465E-545.039
ATACCGG1000.045.02
GTACGAT406.8212103E-945.017
CCCGATA453.8562575E-1045.020
ATCGTTC650.044.99999640
CCGTCGA650.044.99999615
TCGATTG1200.043.1250041
GCGTAAG1850.042.5675661
Produced by FastQC (version 0.11.5)