##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545267_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3579702
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.307930660149925	31.0	31.0	33.0	30.0	34.0
2	31.628690041796776	31.0	31.0	34.0	30.0	34.0
3	31.712680552738746	31.0	31.0	34.0	30.0	34.0
4	30.28296601225465	35.0	33.0	37.0	0.0	37.0
5	32.86115352618737	35.0	33.0	37.0	19.0	37.0
6	34.49563203864456	35.0	33.0	37.0	30.0	37.0
7	35.538902120902804	36.0	35.0	37.0	33.0	37.0
8	35.73167459190737	37.0	35.0	37.0	35.0	37.0
9	37.7725003366202	39.0	37.0	39.0	35.0	39.0
10	37.16145841190133	39.0	37.0	39.0	33.0	39.0
11	36.7097632707974	39.0	35.0	39.0	32.0	39.0
12	35.630875419238805	37.0	35.0	39.0	31.0	39.0
13	35.21065775866259	37.0	35.0	39.0	30.0	39.0
14	36.03394863594791	38.0	35.0	40.0	30.0	41.0
15	36.381703281446335	38.0	35.0	40.0	31.0	41.0
16	36.578587826584446	38.0	35.0	40.0	32.0	41.0
17	36.53657120061949	38.0	35.0	40.0	32.0	41.0
18	36.491235583297154	37.0	35.0	40.0	31.0	41.0
19	36.480816559590714	37.0	35.0	40.0	31.0	41.0
20	36.373254533477926	37.0	35.0	40.0	31.0	41.0
21	36.191686347075816	37.0	35.0	40.0	31.0	41.0
22	36.06541578041971	37.0	34.0	40.0	30.0	41.0
23	36.058373015407426	37.0	34.0	40.0	31.0	41.0
24	35.989329000011736	37.0	34.0	40.0	31.0	41.0
25	35.89348442970951	36.0	34.0	40.0	30.0	41.0
26	35.753700168338035	36.0	34.0	40.0	30.0	41.0
27	35.728163685133566	36.0	34.0	40.0	30.0	41.0
28	35.782290257680664	36.0	34.0	40.0	30.0	41.0
29	35.83727081192792	36.0	34.0	40.0	30.0	41.0
30	35.783295927985066	36.0	34.0	40.0	30.0	41.0
31	35.66625043090179	36.0	34.0	40.0	30.0	41.0
32	35.573616742399224	36.0	34.0	40.0	30.0	41.0
33	35.44049141520719	36.0	34.0	40.0	29.0	41.0
34	35.3784669785362	36.0	34.0	40.0	29.0	41.0
35	35.24595064058405	36.0	34.0	40.0	29.0	41.0
36	35.04637872091029	36.0	34.0	40.0	27.0	41.0
37	34.98326201454758	36.0	34.0	40.0	27.0	41.0
38	34.975776195895634	35.0	34.0	40.0	28.0	41.0
39	34.968873945373105	36.0	34.0	40.0	28.0	41.0
40	34.879082951597645	36.0	34.0	40.0	27.0	41.0
41	34.885906983318726	36.0	34.0	40.0	27.0	41.0
42	34.83027022919785	35.0	34.0	40.0	27.0	41.0
43	34.739915221993336	35.0	33.0	40.0	27.0	41.0
44	34.65823775275149	35.0	33.0	40.0	27.0	41.0
45	34.56647452776795	35.0	33.0	40.0	27.0	41.0
46	34.55548925580956	35.0	33.0	40.0	27.0	41.0
47	34.492158844507166	35.0	33.0	40.0	27.0	41.0
48	34.409165343930866	35.0	33.0	39.0	27.0	41.0
49	34.40876503127914	35.0	33.0	39.0	27.0	41.0
50	34.23036107474868	35.0	33.0	39.0	26.0	41.0
51	34.14469835757278	35.0	33.0	39.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	1.0
12	2.0
13	6.0
14	12.0
15	56.0
16	200.0
17	553.0
18	1346.0
19	2690.0
20	4915.0
21	8135.0
22	12683.0
23	18432.0
24	26448.0
25	36256.0
26	47409.0
27	58774.0
28	71007.0
29	86761.0
30	108279.0
31	137630.0
32	177198.0
33	260101.0
34	391233.0
35	535064.0
36	255839.0
37	324738.0
38	438424.0
39	575174.0
40	334.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.916064521571908	25.853436962071147	29.574752311784614	12.655746204572335
2	27.63889284638777	26.86262711253618	31.70872882714818	13.789751213927865
3	28.62780197904742	26.01772438040932	31.76116335940813	13.593310281135135
4	22.168074325739966	39.08775087982184	26.527012583729036	12.217162210709159
5	24.31582293721656	32.15862661193585	29.451529764209422	14.07402068663816
6	21.619090080682692	41.15445922593557	26.174189918602163	11.052260774779576
7	90.65480869636635	3.7248631310650997	3.9211364521404293	1.699191720428125
8	92.87122224140445	2.0793630307774222	3.328042390120742	1.7213723376973837
9	89.48786798454172	3.8521362951441205	4.464589510523502	2.1954062097906473
10	56.45609606609712	25.727113597724056	8.716451816380246	9.100338519798576
11	47.91273128321855	19.324876763484784	20.09343235833597	12.668959594960699
12	43.2085966932443	22.038901562197076	21.3231715936131	13.429330150945527
13	20.049825376525757	43.707688517088854	21.41491107360333	14.82757503278206
14	13.809250043718723	44.26293026626239	26.685824685965482	15.241995004053411
15	12.759497857642899	24.652191718752007	48.246418277275595	14.3418921463295
16	15.562831766443127	19.66867633115829	46.20429298304719	18.564198919351387
17	16.70183719203442	20.534949557253647	26.444352071764634	36.3188611789473
18	21.613866182157064	24.289284415294905	32.782421553525964	21.31442784902207
19	30.24162346474651	25.76362501683101	23.5490552006843	20.44569631773818
20	33.77395660309154	24.375213355748606	23.887770546263347	17.963059494896502
21	22.799495600471772	29.49292985840721	26.944756854062152	20.762817687058867
22	23.07949097438837	25.37878851368075	25.233999925133432	26.30772058679745
23	20.644874908581777	31.483598355393827	23.927718005577	23.943808730447394
24	19.94149233651293	24.212462378153266	38.68590178735548	17.16014349797832
25	17.379882459489647	27.362612865540203	34.588912708376284	20.668591966593866
26	16.835507536660874	38.378669509361394	25.11932557514564	19.666497378832094
27	17.558835903100313	35.97662598730285	28.433176839859854	18.03136126973698
28	14.95493200271978	29.644450850936753	39.0017940040819	16.398823142261563
29	15.285182956570129	25.40024281350794	38.15496373720494	21.15961049271699
30	18.33004535014367	32.820692895665616	30.250115791761438	18.599145962429276
31	29.114043571224645	28.366551182193376	23.528075800723077	18.991329445858902
32	31.25176341494348	27.63207663654684	23.806646475041777	17.309513473467902
33	27.959087097194125	28.8644417887299	23.79834969503048	19.378121419045495
34	18.966634652828642	27.77700490152532	27.75817651860406	25.49818392704197
35	20.044098642847924	26.87754455538478	30.9126011047847	22.165755696982597
36	31.547793643158005	25.241542452416432	25.324454381956933	17.88620952246863
37	20.776757394889295	32.60394300978126	28.991128311798022	17.628171283531422
38	19.494024921627553	33.78468375300514	25.286127169244814	21.435164156122493
39	20.42527562350162	32.974504581666295	26.30649143420318	20.2937283606289
40	25.26221456422909	27.059598815767345	24.179694287401578	23.498492332601987
41	17.703847973937496	24.33420435555809	27.14974598444228	30.812201686062135
42	21.977639479487397	26.016942192394787	23.94009333737836	28.065324990739455
43	21.421392060009463	28.094880523574307	25.482679843182478	25.00104757323375
44	19.329011185847314	31.322607300831184	28.543744702771345	20.80463681055015
45	16.81908158835568	38.02908733743758	22.942831554134955	22.20899952007178
46	21.505225853995668	33.999534039425626	25.157904205433862	19.337335901144844
47	20.708343878903886	27.742700370030803	27.593861164979653	23.955094586085657
48	23.04742126579252	23.662779750940164	32.01129591234131	21.278503070926018
49	20.55632005122214	24.26014791175355	32.43566084551172	22.74787119151259
50	18.72762034381633	34.079792116773966	26.931180304952758	20.261407234456946
51	17.77533437140857	35.41372996970139	24.23360938983189	22.57732626905815
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	541.0
1	1192.5
2	1844.0
3	5246.0
4	8648.0
5	6549.5
6	4451.0
7	5016.0
8	5581.0
9	6085.5
10	6590.0
11	6852.5
12	7115.0
13	7102.5
14	7090.0
15	7194.5
16	7299.0
17	7061.5
18	6824.0
19	9022.0
20	11220.0
21	11477.5
22	11735.0
23	14204.0
24	16673.0
25	21406.5
26	31485.5
27	36831.0
28	42285.5
29	47740.0
30	61688.0
31	75636.0
32	84540.5
33	93445.0
34	104430.5
35	115416.0
36	125209.0
37	135002.0
38	157467.5
39	179933.0
40	243896.0
41	307859.0
42	356331.5
43	404804.0
44	393356.0
45	381908.0
46	351346.0
47	320784.0
48	294129.5
49	267475.0
50	251945.5
51	236416.0
52	215479.5
53	194543.0
54	171127.0
55	147711.0
56	132922.5
57	118134.0
58	110818.5
59	103503.0
60	94717.0
61	85931.0
62	76848.5
63	67766.0
64	56007.0
65	44248.0
66	40626.0
67	37004.0
68	29028.0
69	21052.0
70	18301.0
71	15550.0
72	12607.5
73	9665.0
74	7367.5
75	3607.0
76	2144.0
77	1542.5
78	941.0
79	953.0
80	965.0
81	608.5
82	252.0
83	208.5
84	165.0
85	102.0
86	39.0
87	23.5
88	8.0
89	5.5
90	3.0
91	5.0
92	7.0
93	3.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3579702.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.185125987238042
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.22666429927762	10.954699626593914
2	10.097861691689804	2.8647888059664908
3	3.7388567737713005	1.591084631525528
4	1.8377180705746017	1.0427304944049895
5	1.0042552653702388	0.7122743731312006
6	0.6409246205463436	0.5454957894463571
7	0.41222445326374896	0.4093219063196619
8	0.28815285285674497	0.3269987617083995
9	0.21759059172918957	0.2777894961584607
>10	1.780955051860038	5.6681105377244725
>50	0.686968872669399	7.141613106681041
>100	2.0280532751180904	64.47466106985065
>500	0.03611925650727079	3.2314732757415134
>1k	0.0036549247656166876	0.7589581247473393
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3970	0.11090308634629363	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.061876659006811184	0.0
2	0.0	0.0	0.0	0.2120567577971574	0.0
3	0.0	0.0	0.0	0.3068691192730568	0.0
4	0.0	0.0	0.0	0.4850124395829597	0.0
5	2.793528623332333E-5	0.0	0.0	0.7971613279541147	0.0
6	2.793528623332333E-5	0.0	0.0	1.1080531284447699	0.0
7	2.793528623332333E-5	0.0	0.0	1.2750223342613436	0.0
8	2.793528623332333E-5	0.0	0.0	1.6561993149150405	0.0
9	2.793528623332333E-5	0.0	0.0	1.767521430554834	0.0
10	2.793528623332333E-5	0.0	0.0	1.9559449361985999	0.0
11	2.793528623332333E-5	0.0	0.0	2.27225618221852	0.0
12	2.793528623332333E-5	0.0	0.0	2.5167458073325655	0.0
13	2.793528623332333E-5	0.0	0.0	2.61630716746813	0.0
14	2.793528623332333E-5	0.0	0.0	2.655360697622316	0.0
15	2.793528623332333E-5	0.0	0.0	2.7175446447776936	0.0
16	2.793528623332333E-5	0.0	0.0	2.8713842660646054	0.0
17	2.793528623332333E-5	0.0	0.0	3.0615956300273037	0.0
18	2.793528623332333E-5	0.0	0.0	3.2784293217703597	0.0
19	2.793528623332333E-5	0.0	0.0	3.4120717311105784	0.0
20	2.793528623332333E-5	0.0	0.0	3.557698378244893	0.0
21	2.793528623332333E-5	0.0	0.0	3.7431328082616933	0.0
22	2.793528623332333E-5	0.0	0.0	3.9309696728945593	0.0
23	2.793528623332333E-5	0.0	0.0	4.146015506318682	0.0
24	2.793528623332333E-5	0.0	0.0	4.298653910297561	0.0
25	2.793528623332333E-5	0.0	0.0	4.42489346878595	0.0
26	2.793528623332333E-5	0.0	0.0	4.553395785459236	0.0
27	2.793528623332333E-5	0.0	0.0	4.676338980172092	0.0
28	2.793528623332333E-5	0.0	0.0	4.810903253958011	0.0
29	5.587057246664666E-5	0.0	0.0	4.9547979133458595	0.0
30	5.587057246664666E-5	0.0	0.0	5.135623021134162	0.0
31	5.587057246664666E-5	0.0	0.0	5.303374414965268	0.0
32	5.587057246664666E-5	0.0	0.0	5.443777163573951	0.0
33	5.587057246664666E-5	0.0	0.0	5.591079927882265	0.0
34	5.587057246664666E-5	0.0	0.0	5.756792045818339	0.0
35	5.587057246664666E-5	0.0	0.0	5.970748403079362	0.0
36	5.587057246664666E-5	0.0	0.0	6.144785236312967	0.0
37	5.587057246664666E-5	0.0	0.0	6.31259250071654	0.0
38	5.587057246664666E-5	0.0	0.0	6.485930951794312	0.0
39	5.587057246664666E-5	0.0	0.0	6.686841530384373	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCTCAT	30	2.166793E-6	45.000004	26
CGTCGGT	30	2.166793E-6	45.000004	21
GACCGTT	35	1.212884E-7	45.0	26
TCTACGA	55	1.8189894E-12	45.0	15
GTTCGAC	20	7.0352876E-4	45.0	42
TACGAGT	25	3.892465E-5	45.0	17
CTACGAC	35	1.212884E-7	45.0	23
CACTACG	20	7.0352876E-4	45.0	38
TACGACA	25	3.892465E-5	45.0	33
TCGCTCA	25	3.892465E-5	45.0	19
ACTACGT	35	1.212884E-7	45.0	21
GATATCG	25	3.892465E-5	45.0	1
CGGTCAT	25	3.892465E-5	45.0	39
ATACCGG	100	0.0	45.0	2
GTACGAT	40	6.8212103E-9	45.0	17
CCCGATA	45	3.8562575E-10	45.0	20
ATCGTTC	65	0.0	44.999996	40
CCGTCGA	65	0.0	44.999996	15
TCGATTG	120	0.0	43.125004	1
GCGTAAG	185	0.0	42.567566	1
>>END_MODULE