FastQCFastQC Report
Thu 26 May 2016
SRR1545266_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545266_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1758434
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21810.12403081378089824No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG19670.1118608944094575No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAC351.212411E-745.00000441
CGCTAAA351.212411E-745.00000412
AAACCGT351.212411E-745.00000425
CCGTTGT351.212411E-745.00000423
AGCGTAA207.0343085E-445.042
CGTATGA207.0343085E-445.033
CGTATCA207.0343085E-445.037
ACGCATC207.0343085E-445.012
CACGCGT253.891653E-545.028
GTCTAGA253.891653E-545.033
TAAGTCG207.0343085E-445.01
TATGATC207.0343085E-445.010
ATCCGTT207.0343085E-445.043
CCGTATG253.891653E-545.01
ACGATAT207.0343085E-445.018
CCCGTAG207.0343085E-445.01
TACGATG253.891653E-545.026
CTACGCG207.0343085E-445.01
TATTACG502.1827873E-1145.01
CGAATCT207.0343085E-445.030