FastQCFastQC Report
Thu 26 May 2016
SRR1545265_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545265_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2939814
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41640.14164161406129777No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATA207.035081E-445.00000434
CCTTACG351.212793E-745.00000438
CGAACTA302.1666601E-645.00000414
ACCGAAT302.1666601E-645.00000434
CATTACG302.1666601E-645.00000435
CCGTATC406.8212103E-945.00000445
GAACGTA302.1666601E-645.00000414
TCCGTTA302.1666601E-645.00000410
CGTACGT302.1666601E-645.00000433
TCCGTCG351.212793E-745.00000425
CTATTCG207.035081E-445.0000047
CGTAAGA207.035081E-445.00000422
ATACGAT207.035081E-445.00000410
TATCGTA302.1666601E-645.0000041
GTAACGT406.8212103E-945.00000432
ATCGATA207.035081E-445.00000411
TTACGTC253.8922946E-545.016
TATATCG950.042.631581
CTATACG800.042.1875041
TATGCGT700.041.78571720