##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545265_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2939814
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.229082180029078	31.0	31.0	33.0	30.0	34.0
2	31.55540044370154	31.0	31.0	34.0	30.0	34.0
3	31.653286228312403	31.0	31.0	34.0	30.0	34.0
4	30.29717764457207	35.0	33.0	37.0	0.0	37.0
5	32.848824109280386	35.0	33.0	37.0	19.0	37.0
6	34.44787799500241	35.0	33.0	37.0	30.0	37.0
7	35.473815010065266	36.0	35.0	37.0	33.0	37.0
8	35.65575883372213	37.0	35.0	37.0	35.0	37.0
9	37.674254901840726	39.0	37.0	39.0	35.0	39.0
10	37.10019953643326	39.0	37.0	39.0	33.0	39.0
11	36.65135515376143	39.0	35.0	39.0	32.0	39.0
12	35.58073163812404	37.0	35.0	39.0	31.0	39.0
13	35.13203182242142	37.0	35.0	39.0	30.0	39.0
14	35.87206605587973	38.0	34.0	40.0	29.0	41.0
15	36.21345704184006	38.0	35.0	40.0	30.0	41.0
16	36.407392440474126	38.0	35.0	40.0	31.0	41.0
17	36.351654220301015	38.0	35.0	40.0	31.0	41.0
18	36.30397025117916	37.0	35.0	40.0	31.0	41.0
19	36.30474819155226	37.0	35.0	40.0	31.0	41.0
20	36.19728424995595	37.0	35.0	40.0	31.0	41.0
21	36.034617496208945	37.0	34.0	40.0	30.0	41.0
22	35.94466826812853	37.0	34.0	40.0	30.0	41.0
23	35.905757643170624	37.0	34.0	40.0	30.0	41.0
24	35.824290244212726	37.0	34.0	40.0	30.0	41.0
25	35.69171791140528	36.0	34.0	40.0	30.0	41.0
26	35.52336848521709	36.0	34.0	40.0	30.0	41.0
27	35.5302692619329	36.0	34.0	40.0	30.0	41.0
28	35.53273540434871	36.0	34.0	40.0	29.0	41.0
29	35.57363935269374	36.0	34.0	40.0	30.0	41.0
30	35.504220335028	36.0	34.0	40.0	29.0	41.0
31	35.34246316263546	36.0	34.0	40.0	29.0	41.0
32	35.262627159405326	36.0	34.0	40.0	29.0	41.0
33	35.127715562957384	36.0	34.0	40.0	29.0	41.0
34	35.02028257570037	36.0	34.0	40.0	27.0	41.0
35	34.88532369734956	35.0	33.0	40.0	27.0	41.0
36	34.70901764533402	35.0	33.0	40.0	26.0	41.0
37	34.62765501490911	35.0	33.0	40.0	26.0	41.0
38	34.59164933563824	35.0	33.0	40.0	27.0	41.0
39	34.508595101594864	35.0	33.0	40.0	26.0	41.0
40	34.36282295410526	35.0	33.0	40.0	25.0	41.0
41	34.32085941491537	35.0	33.0	40.0	25.0	41.0
42	34.274820787981824	35.0	33.0	39.0	26.0	41.0
43	34.176858127759104	35.0	33.0	39.0	26.0	41.0
44	34.096467327524806	35.0	33.0	39.0	26.0	41.0
45	34.005300335327334	35.0	33.0	39.0	25.0	41.0
46	33.97385923054996	35.0	33.0	39.0	25.0	41.0
47	33.87180073297154	35.0	33.0	39.0	24.0	41.0
48	33.7627894145684	35.0	33.0	39.0	24.0	41.0
49	33.70540755299485	35.0	33.0	39.0	24.0	40.0
50	33.552127447518785	35.0	32.0	38.0	24.0	40.0
51	33.46034238900828	35.0	32.0	38.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	2.0
11	1.0
12	6.0
13	6.0
14	40.0
15	85.0
16	326.0
17	761.0
18	1711.0
19	3274.0
20	5561.0
21	8887.0
22	13086.0
23	18638.0
24	25676.0
25	34806.0
26	44308.0
27	54681.0
28	65551.0
29	78849.0
30	96340.0
31	120268.0
32	151752.0
33	217370.0
34	321150.0
35	417074.0
36	229104.0
37	282957.0
38	351733.0
39	395626.0
40	183.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.52678570821147	25.002330079385978	30.119966773408112	12.35091743899444
2	28.703686695824977	26.804859082921574	30.44076938200852	14.050684839244932
3	29.311956470715494	25.712306969080355	30.776130734801587	14.199605825402559
4	22.355563991463406	38.479645310893815	25.656078921999825	13.508711775642949
5	24.17938685916864	32.06491975342658	28.979180315489344	14.776513071915435
6	22.0822813960339	40.97017702480497	25.8232663699132	11.124275209247932
7	89.92004256051574	3.988007404550084	4.281121186578471	1.8108288483557122
8	91.83955175395451	2.2770828358528803	3.783130497371603	2.100234912821015
9	88.10390725399634	3.703227483099271	5.499225461202648	2.69363980170174
10	59.43413426835848	19.630085440779588	9.966242762297206	10.969537528564732
11	49.88159114828353	20.669606988741464	15.953764421830769	13.495037441144236
12	43.89906980509652	20.81475222582109	19.93721371488128	15.348964254201118
13	23.691975070531672	36.941384727060964	21.660179861719143	17.70646034068822
14	17.443756645828614	38.775480353518965	26.403439129142182	17.37732387151024
15	15.979276239925383	22.639085329888218	44.38063088345045	17.00100754673595
16	20.36190044676296	18.27704065631363	41.63011673527645	19.73094216164696
17	20.89210405828396	19.379185213758422	25.426642637935597	34.30206809002202
18	25.176354694548703	23.70289412867617	30.09309432501512	21.02765685176001
19	32.94014519285914	24.547199244578056	21.664227736856823	20.848427825705983
20	35.30570301386414	24.292217126661754	21.660588050808656	18.741491808665447
21	26.655665970704266	27.43853862863433	25.50620549463333	20.39958990602807
22	26.142844411245065	23.71265665106704	24.029785557861825	26.11471337982607
23	24.442294648573004	28.14586228924687	23.15211098389218	24.259732078287946
24	24.096830615814472	23.359403009850283	33.524910079345155	19.01885629499009
25	22.2542310499916	24.805106717635876	30.81324192618989	22.127420306182636
26	20.568648220601712	34.027731006111274	24.118838810890757	21.28478196239626
27	21.890840713051915	31.139963276588244	26.926193289779558	20.043002720580283
28	18.79408016969781	27.290740162472865	34.517932086859915	19.39724758096941
29	19.698355066000776	25.23142620587561	33.071616095440056	21.998602632683564
30	23.652924980968184	27.69498342412139	27.851013703588052	20.801077891322375
31	31.275311975519536	25.220541163488576	22.939342420983095	20.564804440008793
32	34.25550051805998	24.958109594688644	22.61520626815166	18.17118361909971
33	31.008084184917823	25.880991110321943	22.47220402379198	20.63872068096825
34	22.59462673488867	25.862350475234148	26.720193862604912	24.82282892727227
35	23.52152210990219	25.11516034687909	28.350467070365674	23.012850472853046
36	32.38027984083347	23.650611909460938	23.89977733285167	20.06933091685392
37	23.820894791303125	30.672858895154594	25.442698075456477	20.06354823808581
38	24.23908451351004	30.859129183002732	21.58585543167017	23.315930871817063
39	23.647448444017208	29.150619733085154	24.88878548098621	22.313146341911427
40	27.477282576380684	24.046725405076646	23.76626548482319	24.709726533719483
41	20.401018567841366	22.491116784939454	26.480927024634894	30.62693762258429
42	24.782588286197697	24.064753756530173	23.162791931734457	27.98986602553767
43	23.45743642284852	24.651729667251058	25.35330466485295	26.537529245047477
44	22.267633258430635	28.28468059543903	27.066916478389448	22.380769667740886
45	20.549837506726615	32.57437375289729	22.25620396392425	24.619584776451845
46	23.966176091412585	29.111875785338796	24.834428300565957	22.087519822682662
47	23.10033900103884	24.823271132119242	26.72968425893611	25.346705607905808
48	24.841673656904824	21.629429616975766	29.659461448921597	23.86943527719781
49	23.555401804331837	22.173001421178345	30.112381259494647	24.159215514995168
50	21.119907585990134	30.573328788828135	24.843680586594935	23.463083038586795
51	19.820097461948272	32.46929907810494	22.82678427954966	24.883819180397126
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	665.0
1	1089.5
2	1514.0
3	4973.0
4	8432.0
5	6103.0
6	3774.0
7	4076.5
8	4379.0
9	4783.5
10	5188.0
11	5202.0
12	5216.0
13	5323.0
14	5430.0
15	5232.5
16	5035.0
17	4939.5
18	4844.0
19	4988.5
20	5133.0
21	6371.0
22	7609.0
23	7834.5
24	8060.0
25	8203.0
26	12381.0
27	16416.0
28	20393.0
29	24370.0
30	26526.5
31	28683.0
32	35260.0
33	41837.0
34	51597.0
35	61357.0
36	67255.0
37	73153.0
38	88021.5
39	102890.0
40	148073.0
41	193256.0
42	223645.0
43	254034.0
44	258304.0
45	262574.0
46	247039.5
47	231505.0
48	227688.5
49	223872.0
50	208623.0
51	193374.0
52	181724.5
53	170075.0
54	161812.0
55	153549.0
56	151559.5
57	149570.0
58	150143.5
59	150717.0
60	141164.5
61	131612.0
62	126838.5
63	122065.0
64	106847.5
65	91630.0
66	79764.5
67	67899.0
68	54066.0
69	40233.0
70	36018.5
71	31804.0
72	25720.0
73	19636.0
74	16182.0
75	10767.5
76	8807.0
77	6630.0
78	4453.0
79	3276.5
80	2100.0
81	1620.0
82	1140.0
83	763.5
84	387.0
85	321.0
86	255.0
87	190.5
88	126.0
89	84.0
90	42.0
91	40.0
92	38.0
93	19.0
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2939814.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.113718202360815
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.10842219689673	12.586171045118183
2	9.129624715117624	2.9422439990542806
3	3.1562905646333914	1.5257873016981833
4	1.587579451398787	1.0232723161479453
5	0.9174506301724129	0.7391770459588305
6	0.6134526352354712	0.5931001736808014
7	0.4350635287118692	0.4907343771251441
8	0.303102933583467	0.3907292206458299
9	0.25737746846304704	0.37325771986054934
>10	2.406102219384558	9.166756772661394
>50	1.1378872424381923	13.445096473037491
>100	1.9363510329976357	55.28166682955645
>500	0.009451237135481768	0.9705993080069015
>1k	0.0018441438313135155	0.47140741744797504
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4164	0.14164161406129777	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.4015757459485534E-5	0.0	0.0	0.06262300948291287	0.0
2	3.4015757459485534E-5	0.0	0.0	0.21270053139416303	0.0
3	3.4015757459485534E-5	0.0	0.0	0.30525740744142316	0.0
4	3.4015757459485534E-5	0.0	0.0	0.49901116193065276	0.0
5	3.4015757459485534E-5	0.0	0.0	0.8320254274590161	0.0
6	3.4015757459485534E-5	0.0	0.0	1.1959940322755114	0.0
7	3.4015757459485534E-5	0.0	0.0	1.3896457394923625	0.0
8	3.4015757459485534E-5	0.0	0.0	1.8289932628390775	0.0
9	3.4015757459485534E-5	0.0	0.0	1.954783533924255	0.0
10	3.4015757459485534E-5	0.0	0.0	2.166123435020039	0.0
11	3.4015757459485534E-5	0.0	0.0	2.5005663623617003	0.0
12	3.4015757459485534E-5	0.0	0.0	2.743642965167184	0.0
13	6.803151491897107E-5	0.0	0.0	2.8432411030085576	0.0
14	6.803151491897107E-5	0.0	0.0	2.886917335586537	0.0
15	6.803151491897107E-5	0.0	0.0	2.9543365668712376	0.0
16	6.803151491897107E-5	0.0	0.0	3.101012513036539	0.0
17	6.803151491897107E-5	0.0	0.0	3.2641520858122317	0.0
18	6.803151491897107E-5	0.0	0.0	3.4516809566863755	0.0
19	6.803151491897107E-5	0.0	0.0	3.571178312641548	0.0
20	6.803151491897107E-5	0.0	0.0	3.6933288976785605	0.0
21	6.803151491897107E-5	0.0	0.0	3.8637138267931235	0.0
22	6.803151491897107E-5	0.0	0.0	4.035765528023202	0.0
23	6.803151491897107E-5	0.0	0.0	4.2143482546855005	0.0
24	6.803151491897107E-5	0.0	0.0	4.3522481354262545	0.0
25	1.020472723784566E-4	0.0	0.0	4.471065176232238	0.0
26	1.020472723784566E-4	0.0	0.0	4.5852560740237305	0.0
27	1.020472723784566E-4	0.0	0.0	4.706726343911553	0.0
28	1.020472723784566E-4	0.0	0.0	4.832210473179596	0.0
29	1.020472723784566E-4	0.0	0.0	4.971334921188892	0.0
30	1.020472723784566E-4	0.0	0.0	5.136821581229289	0.0
31	1.3606302983794214E-4	0.0	0.0	5.2954710740203295	0.0
32	1.3606302983794214E-4	0.0	0.0	5.43694260929433	0.0
33	1.3606302983794214E-4	0.0	0.0	5.575658868214112	0.0
34	1.3606302983794214E-4	0.0	0.0	5.734682534337207	0.0
35	1.3606302983794214E-4	0.0	0.0	5.917857388256536	0.0
36	1.3606302983794214E-4	0.0	0.0	6.0805207404277954	0.0
37	1.7007878729742766E-4	0.0	0.0	6.25349086710928	0.0
38	2.040945447569132E-4	0.0	0.0	6.440645564651369	0.0
39	2.040945447569132E-4	0.0	0.0	6.734813835161	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCATA	20	7.035081E-4	45.000004	34
CCTTACG	35	1.212793E-7	45.000004	38
CGAACTA	30	2.1666601E-6	45.000004	14
ACCGAAT	30	2.1666601E-6	45.000004	34
CATTACG	30	2.1666601E-6	45.000004	35
CCGTATC	40	6.8212103E-9	45.000004	45
GAACGTA	30	2.1666601E-6	45.000004	14
TCCGTTA	30	2.1666601E-6	45.000004	10
CGTACGT	30	2.1666601E-6	45.000004	33
TCCGTCG	35	1.212793E-7	45.000004	25
CTATTCG	20	7.035081E-4	45.000004	7
CGTAAGA	20	7.035081E-4	45.000004	22
ATACGAT	20	7.035081E-4	45.000004	10
TATCGTA	30	2.1666601E-6	45.000004	1
GTAACGT	40	6.8212103E-9	45.000004	32
ATCGATA	20	7.035081E-4	45.000004	11
TTACGTC	25	3.8922946E-5	45.0	16
TATATCG	95	0.0	42.63158	1
CTATACG	80	0.0	42.187504	1
TATGCGT	70	0.0	41.785717	20
>>END_MODULE