FastQCFastQC Report
Thu 26 May 2016
SRR1545264_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545264_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2682001
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29930.1115957824027657No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC28420.10596565773092553No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTC700.045.00000427
CTGTCCG351.2127384E-745.00000439
ACGTTAG551.8189894E-1245.0000041
ACATTCG551.8189894E-1245.0000041
CGCGCGA351.2127384E-745.00000415
TCGCTTA351.2127384E-745.00000433
CTTGCAC650.045.00000424
TGTTACG1400.045.0000041
TCCGTCG351.2127384E-745.00000423
ACCTTCG351.2127384E-745.00000422
TCGTTCC253.892202E-545.028
CGTATTG453.8562575E-1045.037
ACGTTGT800.045.039
ACGTTAC207.034971E-445.018
CGCATCG207.034971E-445.021
CAACCTA207.034971E-445.038
CACGCGA253.892202E-545.039
CCGTGAT406.8212103E-945.020
CTCCACG253.892202E-545.013
ATCGTTC253.892202E-545.010