FastQCFastQC Report
Thu 26 May 2016
SRR1545263_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545263_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4071574
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG55700.13680213106773942No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48280.11857822060952349No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTA800.045.035
CGACGAA502.1827873E-1145.034
TACGAAC253.8925595E-545.011
CGTAATC207.0354046E-445.026
CGTGTAA207.0354046E-445.041
TTACGTA406.8230293E-945.017
ATACGAT551.8189894E-1245.022
GCGTATT253.8925595E-545.044
TAGTCCG253.8925595E-545.01
GCGTAAT207.0354046E-445.044
TAATCGC207.0354046E-445.026
TCTACGT302.1668675E-644.99999616
CGAATCG302.1668675E-644.99999615
TTAACGA600.044.99999642
CGGTCGA302.1668675E-644.99999631
CGGTCTA1450.043.4482831
CGTTAGG5900.042.7118642
CGGGTAT4500.042.56
ATACCCG850.042.3529429
ACGGGTA3300.042.2727285
Produced by FastQC (version 0.11.5)