##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545263_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4071574
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.256389543699807	31.0	31.0	33.0	30.0	34.0
2	31.581777956141778	31.0	31.0	34.0	30.0	34.0
3	31.677462819047374	31.0	31.0	34.0	30.0	34.0
4	30.28249861110224	35.0	33.0	37.0	0.0	37.0
5	32.837374440449814	35.0	33.0	37.0	19.0	37.0
6	34.46174305072191	35.0	33.0	37.0	30.0	37.0
7	35.4807008297037	36.0	35.0	37.0	33.0	37.0
8	35.66301165102243	37.0	35.0	37.0	35.0	37.0
9	37.691792903678035	39.0	37.0	39.0	35.0	39.0
10	37.14174346333875	39.0	37.0	39.0	33.0	39.0
11	36.70736845259352	39.0	35.0	39.0	32.0	39.0
12	35.55259317404031	37.0	35.0	39.0	31.0	39.0
13	35.08039249685748	37.0	35.0	39.0	30.0	39.0
14	35.90794690210715	37.0	35.0	40.0	30.0	41.0
15	36.23648446522156	37.0	35.0	40.0	31.0	41.0
16	36.43831550157261	37.0	35.0	40.0	32.0	41.0
17	36.362686273171015	37.0	35.0	40.0	31.0	41.0
18	36.343387102874715	37.0	35.0	40.0	31.0	41.0
19	36.32853068616707	37.0	35.0	40.0	31.0	41.0
20	36.2337835441527	37.0	35.0	40.0	31.0	41.0
21	36.05290828559176	37.0	34.0	40.0	31.0	41.0
22	35.926061763828926	37.0	34.0	40.0	30.0	41.0
23	35.89685094756966	36.0	34.0	40.0	30.0	41.0
24	35.81449778390372	36.0	34.0	40.0	30.0	41.0
25	35.713969339621485	36.0	34.0	40.0	30.0	41.0
26	35.52233951783757	36.0	34.0	40.0	30.0	41.0
27	35.53323751453369	36.0	34.0	40.0	30.0	41.0
28	35.54911392989542	36.0	34.0	40.0	30.0	41.0
29	35.60602533565643	36.0	34.0	40.0	30.0	41.0
30	35.575105352377236	36.0	34.0	40.0	30.0	41.0
31	35.44638584488456	36.0	34.0	40.0	30.0	41.0
32	35.35209773910532	36.0	34.0	40.0	29.0	41.0
33	35.22200529819672	36.0	34.0	40.0	29.0	41.0
34	35.11398613902142	36.0	34.0	40.0	29.0	41.0
35	34.98037294667861	35.0	34.0	40.0	28.0	41.0
36	34.819220281886075	35.0	33.0	40.0	27.0	41.0
37	34.76090868052503	35.0	33.0	40.0	27.0	41.0
38	34.74744042475957	35.0	33.0	40.0	27.0	41.0
39	34.684844485204	35.0	33.0	40.0	27.0	41.0
40	34.53744964478111	35.0	33.0	40.0	26.0	41.0
41	34.50112143362739	35.0	33.0	40.0	26.0	41.0
42	34.45770087931596	35.0	33.0	40.0	26.0	41.0
43	34.37682503130239	35.0	33.0	40.0	27.0	41.0
44	34.31856451583589	35.0	33.0	39.0	26.0	41.0
45	34.22306680414012	35.0	33.0	39.0	26.0	41.0
46	34.15671850738805	35.0	33.0	39.0	26.0	41.0
47	34.075178542745384	35.0	33.0	39.0	26.0	41.0
48	33.99487397257179	35.0	33.0	39.0	26.0	41.0
49	33.95437219119682	35.0	33.0	39.0	26.0	41.0
50	33.825082879495746	35.0	33.0	39.0	25.0	40.0
51	33.72258983872085	35.0	33.0	38.0	25.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	3.0
12	3.0
13	5.0
14	16.0
15	87.0
16	304.0
17	820.0
18	1837.0
19	3791.0
20	6503.0
21	10589.0
22	16048.0
23	23895.0
24	32911.0
25	44538.0
26	57935.0
27	71460.0
28	87288.0
29	105942.0
30	130826.0
31	163934.0
32	208987.0
33	304976.0
34	459879.0
35	617554.0
36	295907.0
37	370580.0
38	474304.0
39	580301.0
40	349.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.47898085605223	25.805843145672902	30.660083790691267	12.055092207583602
2	27.678509588675045	28.127868976469543	31.00353818940783	13.190083245447584
3	28.814311123904417	26.355630525197384	30.842519379483218	13.987538971414986
4	22.852292504078275	38.54808975595188	26.05056422896894	12.549053511000904
5	24.126517165106172	33.4695132644034	28.447647027906157	13.956322542584267
6	21.76349981604166	41.902099777629	25.498050631033603	10.836349775295744
7	90.04694498982457	4.086773321570479	4.142280110836743	1.7240015777682045
8	91.67606925478943	2.631734066481415	3.5041730790107217	2.1880235997184383
9	88.55096333752009	4.409375833522859	4.722964632351027	2.31669619660603
10	59.92137193134646	21.95848583373408	8.737284401560675	9.382857833358795
11	52.461824346063715	19.796275347077078	15.103471040929135	12.638429265930078
12	48.88866074888974	19.873125233632006	17.58597535007346	13.652238667404792
13	23.225244094789876	40.94291298647648	18.30803026053315	17.523812658200487
14	14.655929132075213	43.430967974547436	23.566291561936488	18.34681133144086
15	15.174180795928061	23.23855590000329	46.94695466667191	14.64030863739674
16	17.85535520169841	17.21022385937232	46.02912780168063	18.90529313724864
17	17.312125482675743	19.51957645863737	25.79194188783011	37.37635617085677
18	23.046418903352855	24.968844972484845	29.561958102689523	22.422778021472777
19	33.691442179363555	26.1528096996395	20.14896450365387	20.00678361734307
20	37.572815820122635	24.05890203641147	20.372686337028384	17.99559580643751
21	26.39753078293554	28.61311129307732	22.736931712404097	22.25242621158304
22	23.762284561204094	26.935946638818304	22.004070170405843	27.29769862957176
23	24.149849665018984	30.74221419038436	21.617438366587464	23.490497778009193
24	24.24762511991677	22.759895804423547	34.243292643090854	18.74918643256883
25	20.725129888342934	24.59238122652321	31.68666466580246	22.995824219331393
26	19.49113536926997	37.73560298793538	22.82237778313743	19.950883859657225
27	21.388583383232135	34.08750522525195	25.654181896239635	18.869729495276275
28	17.863067206932747	30.386454968029565	34.72585786234021	17.024619962697475
29	18.688423690690627	26.23331910460181	34.1764388907091	20.90181831399847
30	22.226072766944675	30.555898038449016	26.297716804361166	20.92031239024515
31	31.16802003353003	28.057282024101738	19.59844030834267	21.176257634025564
32	34.322623142794406	28.438289467414812	20.16910413515756	17.069983254633218
33	30.797622737545726	29.34575179033956	20.011646601535425	19.84497887057929
34	20.94627286646393	28.82737732385559	25.098180703580482	25.1281691061
35	22.283225111467946	24.772459004797653	27.810424175024206	25.133891708710195
36	30.489216209750825	27.439928636935	22.199154430203162	19.87170072311101
37	22.981480872016572	33.787572079004335	24.538102463568144	18.69284458541095
38	22.85199777776359	32.895239040233584	20.14781015892134	24.104953023081492
39	23.903704071201947	31.05491881026846	23.593946714464725	21.447430404064864
40	27.577934233787722	24.79448979682059	23.40554291780034	24.22203305159135
41	17.825833449177146	24.38720259044782	26.346715054178066	31.440248906196967
42	24.12823640194185	26.729441734326826	20.6316034044819	28.510718459249418
43	23.0248793218544	27.60826648367437	22.322104424480557	27.044749769990673
44	21.69119362683817	30.832842532150956	25.167023858586386	22.308939982424487
45	20.02235499096909	34.689287238792666	19.733031992050247	25.555325778187992
46	22.15671384088807	33.55562246934478	22.055819199159835	22.23184449060732
47	22.53786864735849	26.90622840208725	25.126793716631457	25.4291092339228
48	24.65388078418813	21.03446971613435	29.518707016009042	24.79294248366848
49	20.812884648541328	24.367922577361973	29.76114397036625	25.058048803730447
50	19.321495814640727	32.3459674317598	24.660413884163717	23.672122869435754
51	18.199742900411486	34.58190861814129	21.613877090284987	25.604471391162235
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	802.0
1	1568.5
2	2335.0
3	6203.0
4	10071.0
5	7527.5
6	4984.0
7	5414.0
8	5844.0
9	6419.5
10	6995.0
11	7308.5
12	7622.0
13	7544.5
14	7467.0
15	7397.5
16	7328.0
17	7181.0
18	7034.0
19	6729.5
20	6425.0
21	7218.5
22	8012.0
23	8493.0
24	8974.0
25	12771.0
26	21581.5
27	26595.0
28	33823.0
29	41051.0
30	47837.0
31	54623.0
32	59079.5
33	63536.0
34	71863.0
35	80190.0
36	83480.5
37	86771.0
38	121594.0
39	156417.0
40	222450.5
41	288484.0
42	353297.0
43	418110.0
44	403753.0
45	389396.0
46	360163.5
47	330931.0
48	311658.5
49	292386.0
50	275038.5
51	257691.0
52	240062.0
53	222433.0
54	208944.0
55	195455.0
56	193247.5
57	191040.0
58	201304.0
59	211568.0
60	213070.5
61	214573.0
62	187155.0
63	159737.0
64	131251.5
65	102766.0
66	84542.5
67	66319.0
68	55610.0
69	44901.0
70	38521.0
71	32141.0
72	26087.5
73	20034.0
74	15698.5
75	8659.5
76	5956.0
77	4880.0
78	3804.0
79	2912.0
80	2020.0
81	1269.5
82	519.0
83	334.5
84	150.0
85	140.5
86	131.0
87	72.5
88	14.0
89	9.5
90	5.0
91	3.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4071574.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.851586100527168
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.2219718172313	10.69646791478862
2	10.005426131009562	2.7718204305228675
3	3.5295623502794795	1.4667011037622575
4	1.8544675468275769	1.0274926758206233
5	1.0652028441146237	0.7377374454890068
6	0.691364255523016	0.5745894907322353
7	0.47103961678728107	0.45672520660818433
8	0.33560147816393027	0.3718890216201495
9	0.2512106285248087	0.31316990853410404
>10	1.808812008969993	5.549862347713282
>50	0.7156667270487203	7.3291815840773475
>100	1.9876278825011469	62.16433262014272
>500	0.0544847698694502	4.731622866269668
>1k	0.007368047170957678	1.6588101711342964
>5k	1.9389597818309678E-4	0.14959721278464505
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	5570	0.13680213106773942	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4828	0.11857822060952349	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07977258917558665	0.0
2	0.0	0.0	0.0	0.2502963227488927	0.0
3	0.0	0.0	0.0	0.3474577644910789	0.0
4	0.0	0.0	0.0	0.5945612188308502	0.0
5	0.0	0.0	0.0	0.9388261149128077	0.0
6	0.0	0.0	0.0	1.351492076528635	0.0
7	0.0	0.0	0.0	1.5400677968765888	0.0
8	0.0	0.0	0.0	1.989697350459552	0.0
9	0.0	0.0	0.0	2.116380544722999	0.0
10	0.0	0.0	0.0	2.328387007088659	0.0
11	0.0	0.0	0.0	2.675746529474842	0.0
12	0.0	0.0	0.0	2.9342215074563303	0.0
13	0.0	0.0	0.0	3.0480349859783957	0.0
14	0.0	0.0	0.0	3.0936193226501594	0.0
15	4.912105244802133E-5	0.0	0.0	3.1635431408099177	0.0
16	4.912105244802133E-5	0.0	0.0	3.3240707402100513	0.0
17	7.368157867203199E-5	0.0	0.0	3.4946435948358054	0.0
18	7.368157867203199E-5	0.0	0.0	3.6951311704024046	2.4560526224010664E-5
19	7.368157867203199E-5	0.0	0.0	3.818965343623866	2.4560526224010664E-5
20	7.368157867203199E-5	0.0	0.0	3.93803477475787	2.4560526224010664E-5
21	1.7192368356807466E-4	0.0	0.0	4.107330482019975	2.4560526224010664E-5
22	1.7192368356807466E-4	0.0	0.0	4.292639652380136	2.4560526224010664E-5
23	1.7192368356807466E-4	0.0	0.0	4.484162635874971	2.4560526224010664E-5
24	1.964842097920853E-4	0.0	0.0	4.6286522116508255	2.4560526224010664E-5
25	1.964842097920853E-4	0.0	0.0	4.746272571737613	2.4560526224010664E-5
26	1.964842097920853E-4	0.0	0.0	4.856009002906493	2.4560526224010664E-5
27	1.964842097920853E-4	0.0	0.0	4.957296613054313	2.4560526224010664E-5
28	1.964842097920853E-4	0.0	0.0	5.07877297575827	2.4560526224010664E-5
29	1.964842097920853E-4	0.0	0.0	5.197572241103809	2.4560526224010664E-5
30	1.964842097920853E-4	0.0	0.0	5.349356293168195	2.4560526224010664E-5
31	1.964842097920853E-4	0.0	0.0	5.505438437321783	2.4560526224010664E-5
32	1.964842097920853E-4	0.0	0.0	5.6450159078528355	2.4560526224010664E-5
33	1.964842097920853E-4	0.0	0.0	5.78031984682091	2.4560526224010664E-5
34	1.964842097920853E-4	0.0	0.0	5.926111130486637	2.4560526224010664E-5
35	2.21044736016096E-4	0.0	0.0	6.098182177212056	2.4560526224010664E-5
36	2.21044736016096E-4	0.0	0.0	6.275828463390325	2.4560526224010664E-5
37	2.4560526224010667E-4	0.0	0.0	6.448660886428688	2.4560526224010664E-5
38	2.4560526224010667E-4	0.0	0.0	6.6090165621452535	2.4560526224010664E-5
39	2.4560526224010667E-4	0.0	0.0	6.775414127312926	2.4560526224010664E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATTA	80	0.0	45.0	35
CGACGAA	50	2.1827873E-11	45.0	34
TACGAAC	25	3.8925595E-5	45.0	11
CGTAATC	20	7.0354046E-4	45.0	26
CGTGTAA	20	7.0354046E-4	45.0	41
TTACGTA	40	6.8230293E-9	45.0	17
ATACGAT	55	1.8189894E-12	45.0	22
GCGTATT	25	3.8925595E-5	45.0	44
TAGTCCG	25	3.8925595E-5	45.0	1
GCGTAAT	20	7.0354046E-4	45.0	44
TAATCGC	20	7.0354046E-4	45.0	26
TCTACGT	30	2.1668675E-6	44.999996	16
CGAATCG	30	2.1668675E-6	44.999996	15
TTAACGA	60	0.0	44.999996	42
CGGTCGA	30	2.1668675E-6	44.999996	31
CGGTCTA	145	0.0	43.44828	31
CGTTAGG	590	0.0	42.711864	2
CGGGTAT	450	0.0	42.5	6
ATACCCG	85	0.0	42.35294	29
ACGGGTA	330	0.0	42.272728	5
>>END_MODULE