FastQCFastQC Report
Thu 26 May 2016
SRR1545262_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545262_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2222479
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC27430.12342073873363932No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24720.11122714770308291No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATAG900.045.0000041
AACCGGC253.891985E-545.00000431
CCTTACG253.891985E-545.00000412
GTCGCGA253.891985E-545.00000417
TCCGCTA253.891985E-545.00000430
CACGGTC453.8562575E-1045.00000432
GACCGGC302.16642E-645.00000410
TCTTAAC253.891985E-545.00000421
CGACCGT302.16642E-645.00000442
ATCGTTC453.8562575E-1045.00000427
ATCCGTT302.16642E-645.00000437
CCCGTAC253.891985E-545.00000416
CGGATTA253.891985E-545.00000420
TACGAAC253.891985E-545.00000410
GTACTAC253.891985E-545.00000419
CGTACGT253.891985E-545.00000429
TCCCGTA253.891985E-545.00000415
ACGTCGT253.891985E-545.00000416
CCGAATC600.045.00000422
ACGTAGC253.891985E-545.00000431