FastQCFastQC Report
Thu 26 May 2016
SRR1545261_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545261_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2556745
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT56810.22219658198216874No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCAAG302.1665492E-645.0000041
CGACGTG302.1665492E-645.00000413
GCGAATC302.1665492E-645.00000426
ACGCACC302.1665492E-645.00000445
TGGACGC302.1665492E-645.00000428
ATTACGC302.1665492E-645.00000438
ATTACCG650.045.0000041
CCGTGGC700.045.00000416
AAGCGTG302.1665492E-645.0000043
TAGCCCG351.212702E-745.00000430
ATCGTTC302.1665492E-645.00000420
TCGGAAT302.1665492E-645.00000412
CTTATCG302.1665492E-645.00000422
CGGTGCC302.1665492E-645.00000421
GAACGAT351.212702E-745.00000418
CCGACAT351.212702E-745.00000441
CATCGTA351.212702E-745.00000426
TATAGCG650.045.0000041
TTACGCC351.212702E-745.00000434
ACGTATA650.045.00000427
Produced by FastQC (version 0.11.5)