##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545261_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2556745
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.32527882131382	31.0	31.0	33.0	30.0	34.0
2	31.6354622772314	31.0	31.0	34.0	30.0	34.0
3	31.71504197720148	31.0	31.0	34.0	30.0	34.0
4	30.25963285349145	35.0	33.0	37.0	0.0	37.0
5	32.83819191980428	35.0	33.0	37.0	19.0	37.0
6	34.45731428046207	35.0	33.0	37.0	30.0	37.0
7	35.514158823034755	36.0	35.0	37.0	33.0	37.0
8	35.72691957938707	37.0	35.0	37.0	35.0	37.0
9	37.78786895056019	39.0	37.0	39.0	35.0	39.0
10	37.24559977627804	39.0	37.0	39.0	34.0	39.0
11	36.71027928088253	39.0	35.0	39.0	32.0	39.0
12	35.16862651535448	35.0	35.0	39.0	31.0	39.0
13	34.532714838593606	35.0	33.0	39.0	27.0	39.0
14	35.1959166831264	35.0	33.0	40.0	27.0	41.0
15	35.67583939735875	35.0	34.0	40.0	30.0	41.0
16	35.9958044310246	35.0	34.0	40.0	32.0	41.0
17	35.96490303100231	35.0	34.0	40.0	31.0	41.0
18	35.958440908264215	36.0	35.0	40.0	31.0	41.0
19	35.9257215717641	36.0	35.0	40.0	31.0	41.0
20	35.757895683769796	35.0	34.0	40.0	31.0	41.0
21	35.596629307967746	35.0	34.0	40.0	31.0	41.0
22	35.43721685189567	35.0	34.0	40.0	30.0	41.0
23	35.44195568975397	35.0	34.0	40.0	30.0	41.0
24	35.36172829124531	35.0	34.0	40.0	30.0	41.0
25	35.28706617202732	35.0	34.0	40.0	30.0	41.0
26	35.08779405063861	35.0	34.0	40.0	29.0	41.0
27	35.05140207568608	35.0	34.0	40.0	29.0	41.0
28	35.17594871604325	35.0	34.0	40.0	29.0	41.0
29	35.30281705840825	36.0	34.0	40.0	30.0	41.0
30	35.30769670029667	36.0	34.0	40.0	30.0	41.0
31	35.12148024147891	35.0	34.0	40.0	30.0	41.0
32	34.962984184969564	35.0	34.0	40.0	29.0	41.0
33	34.81053487930944	35.0	34.0	40.0	29.0	41.0
34	34.742542177651664	35.0	34.0	40.0	29.0	41.0
35	34.621682647272216	35.0	34.0	40.0	28.0	41.0
36	34.380243630084344	35.0	33.0	39.0	27.0	41.0
37	34.29778135871978	35.0	33.0	39.0	27.0	41.0
38	34.36336044462784	35.0	33.0	39.0	27.0	41.0
39	34.3488744477842	35.0	33.0	39.0	27.0	41.0
40	34.192822905686725	35.0	33.0	39.0	26.0	41.0
41	34.23931052959916	35.0	33.0	39.0	26.0	41.0
42	34.20685676514474	35.0	33.0	39.0	27.0	41.0
43	34.12536682383265	35.0	33.0	39.0	27.0	41.0
44	34.08860210932259	35.0	33.0	39.0	27.0	41.0
45	34.01760871733396	35.0	33.0	39.0	27.0	41.0
46	33.96438714068083	35.0	33.0	38.0	26.0	41.0
47	33.91016546429151	35.0	33.0	38.0	26.0	41.0
48	33.89902708326407	35.0	33.0	38.0	26.0	40.0
49	33.92880557114613	35.0	33.0	38.0	26.0	40.0
50	33.73079599256086	35.0	33.0	38.0	26.0	40.0
51	33.65911422531383	35.0	33.0	38.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	1.0
13	3.0
14	15.0
15	37.0
16	164.0
17	481.0
18	1139.0
19	2412.0
20	4410.0
21	7216.0
22	11388.0
23	16756.0
24	22913.0
25	30425.0
26	38786.0
27	47058.0
28	54959.0
29	67372.0
30	82772.0
31	105200.0
32	137140.0
33	208941.0
34	330783.0
35	476204.0
36	150306.0
37	179695.0
38	247913.0
39	331976.0
40	278.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.870831858476304	25.5215127046303	32.3680304449603	12.239624991933102
2	26.978325957418512	27.261420282429416	32.36951670972271	13.390737050429355
3	27.793933301913178	26.64677157870652	31.014317032007494	14.544978087372812
4	21.329659391139906	38.607057019765364	27.215971870483756	12.847311718610968
5	23.91869349504937	32.05697087507749	29.640656381453763	14.383679248419377
6	21.20888082307778	41.376672292309166	27.120420691152226	10.294026193460827
7	89.35529354706864	4.174409258647225	4.956028074759118	1.514269119525021
8	92.36806955719088	2.6299846093372627	3.203721919862951	1.798223913608905
9	90.0692873164903	3.2481143015826768	4.369383728138708	2.313214653788313
10	67.34472933358626	17.862047251485777	7.574552800533491	7.218670614394475
11	61.52166133110655	15.472407299124471	14.268024382564551	8.737906987204434
12	56.02572802528215	20.14690553809629	14.635914023494717	9.191452413126846
13	20.81060097897913	52.75578909903022	14.57372557685651	11.859884345134144
14	10.798808641456228	52.86448198784001	22.538892224292997	13.797817146410768
15	9.195128962802313	23.427991450066393	56.353332068704546	11.02354751842675
16	10.250376944122312	14.693643675845655	56.04348497797004	19.012494402061996
17	11.864656037266133	17.115277432829636	26.26116409731905	44.75890243258518
18	19.950366579381203	24.3001159677637	33.45284727260638	22.296670180248714
19	33.37732155533696	25.79021372878406	20.06476985385715	20.76769486202183
20	37.769116591603776	22.554810902143156	20.618716375704267	19.057356130548804
21	21.368302274962893	31.628848399038624	23.779688627532273	23.223160698466213
22	21.415706298438053	27.594656487056785	21.41465026821212	29.57498694629304
23	20.149408720854055	34.79549192430219	20.82616764675398	24.228931708089778
24	20.33894659029352	22.596348091029807	41.508597846089465	15.556107472587216
25	14.702326591036648	26.75910190496119	36.477435176366825	22.06113632763533
26	14.61365916428897	41.94990114383718	24.161580447013684	19.274859244860167
27	16.166180045331075	39.107106887859366	27.84294092684253	16.88377213996703
28	11.361477190724925	30.615215831066457	42.95223027716882	15.071076701039798
29	12.281787976509193	25.391933884685407	41.60880338086122	20.71747475794418
30	17.962800357485786	32.146342321975794	31.04576326540191	18.84509405513651
31	32.813284077997615	27.232672792945717	21.054504848938787	18.899538280117884
32	36.76592698919916	26.478901885013954	21.79517315962288	14.95999796616401
33	31.66991624115819	28.296369016073168	21.27204707548074	18.7616676672879
34	18.303233212541727	27.065780905017906	28.05520300225482	26.57578288018555
35	19.820005514824512	24.55352410975674	31.914289457884927	23.712180917533818
36	32.93382797267619	24.235971909595992	24.221187486432946	18.60901263129487
37	19.861855601555884	34.980962121760285	28.72413948203673	16.4330427946471
38	19.31424526106436	35.545860068172615	22.655407559220805	22.484487111542215
39	20.41677992916775	35.1986607972246	24.455821757742754	19.928737515864896
40	26.1357702860473	25.51044394337331	22.53388585877747	25.81989991180192
41	14.692509421158542	22.830904137878434	27.024556613975975	35.452029826987044
42	22.312979980404773	25.64350375184072	20.437822309225208	31.6056939585293
43	21.866865878294472	28.903273498139235	22.595644070879185	26.634216552687107
44	19.227924568152083	33.69757249940843	26.720967480135876	20.35353545230361
45	15.683535119849653	41.620224152193515	18.5742418583003	24.121998869656537
46	20.889607684771068	36.817594245808635	22.984184969560907	19.308613099859393
47	20.635143512552094	26.57030716790294	27.388104797310643	25.406444522234324
48	23.864288382298586	19.64806814915058	33.23025956831831	23.257383900232522
49	18.53325224064191	22.854136802848934	33.45765807696896	25.1549528795402
50	16.68594247764247	36.71277346782726	25.6144433645123	20.98684069001797
51	14.848919231288221	38.65825492960776	21.704941243651596	24.787884595452418
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	408.0
1	596.5
2	785.0
3	2765.5
4	4746.0
5	3673.0
6	2600.0
7	3052.0
8	3504.0
9	4147.0
10	4790.0
11	4936.0
12	5082.0
13	5041.0
14	5000.0
15	4795.5
16	4591.0
17	4268.5
18	3946.0
19	4633.5
20	5321.0
21	5304.0
22	5287.0
23	5652.5
24	6018.0
25	7668.5
26	12084.5
27	14850.0
28	17484.5
29	20119.0
30	26904.5
31	33690.0
32	40080.0
33	46470.0
34	55729.5
35	64989.0
36	65843.5
37	66698.0
38	94841.5
39	122985.0
40	183084.5
41	243184.0
42	288164.5
43	333145.0
44	321477.0
45	309809.0
46	283503.0
47	257197.0
48	233781.5
49	210366.0
50	198055.5
51	185745.0
52	166539.0
53	147333.0
54	123859.0
55	100385.0
56	91242.0
57	82099.0
58	78951.0
59	75803.0
60	75591.5
61	75380.0
62	63641.0
63	51902.0
64	39650.5
65	27399.0
66	19162.0
67	10925.0
68	8199.0
69	5473.0
70	4579.0
71	3685.0
72	2853.5
73	2022.0
74	1739.5
75	1036.0
76	615.0
77	737.0
78	859.0
79	745.5
80	632.0
81	365.0
82	98.0
83	56.5
84	15.0
85	17.0
86	19.0
87	9.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2556745.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.066632294664313
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.68664562065291	10.927894781516184
2	10.148402769192527	2.8550770026476866
3	3.611565784064572	1.5240770367728207
4	1.759086274253669	0.9897767917806953
5	0.9915900854876595	0.6974166559794829
6	0.6110470621942974	0.5157224603173235
7	0.41204902822409817	0.4057299517181502
8	0.26738297347230605	0.3008942375751129
9	0.19264417738167483	0.24388693262424968
>10	1.6006719055677223	5.072245549903853
>50	0.6390870514174067	6.668010108741823
>100	2.023321718311592	64.0592708929216
>500	0.05046217547161214	4.399816142340754
>1k	0.005741205592578627	1.0987107654436268
>5k	3.021687153988751E-4	0.2414706897166329
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	5681	0.22219658198216874	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06520008839364114	0.0
2	0.0	0.0	0.0	0.22861098779893968	0.0
3	0.0	0.0	0.0	0.3319846132484859	0.0
4	3.911223059006667E-5	0.0	0.0	0.5824593379472728	0.0
5	3.911223059006667E-5	0.0	0.0	0.9839854971848973	0.0
6	3.911223059006667E-5	0.0	0.0	1.3695147541111843	0.0
7	3.911223059006667E-5	0.0	0.0	1.577630933080929	0.0
8	3.911223059006667E-5	0.0	0.0	2.0555432786609535	0.0
9	3.911223059006667E-5	0.0	0.0	2.190284913043733	0.0
10	3.911223059006667E-5	0.0	0.0	2.4513981644630185	0.0
11	3.911223059006667E-5	0.0	0.0	2.7776723920453543	0.0
12	3.911223059006667E-5	0.0	0.0	3.1122775247433747	0.0
13	1.173366917702E-4	0.0	0.0	3.222104668240282	0.0
14	1.173366917702E-4	0.0	0.0	3.2651672341199456	0.0
15	1.173366917702E-4	0.0	0.0	3.3361559326409167	0.0
16	1.173366917702E-4	0.0	0.0	3.4928786406153134	0.0
17	1.173366917702E-4	0.0	0.0	3.6616870278420413	0.0
18	1.173366917702E-4	0.0	0.0	3.8623718829996734	0.0
19	1.173366917702E-4	0.0	0.0	4.00110296490264	0.0
20	1.173366917702E-4	0.0	0.0	4.114450209152653	0.0
21	1.173366917702E-4	0.0	0.0	4.296009183551742	0.0
22	1.5644892236026667E-4	0.0	0.0	4.474947638501297	0.0
23	1.5644892236026667E-4	0.0	0.0	4.683454939776943	0.0
24	1.5644892236026667E-4	0.0	0.0	4.818313910851493	0.0
25	1.9556115295033333E-4	0.0	0.0	4.929588206880233	0.0
26	1.9556115295033333E-4	0.0	0.0	5.032375148870928	0.0
27	1.9556115295033333E-4	0.0	0.0	5.134027836174511	0.0
28	1.9556115295033333E-4	0.0	0.0	5.244598112052629	0.0
29	1.9556115295033333E-4	0.0	0.0	5.367566965027799	0.0
30	1.9556115295033333E-4	0.0	0.0	5.536336240023937	0.0
31	1.9556115295033333E-4	0.0	0.0	5.699043119278614	0.0
32	1.9556115295033333E-4	0.0	0.0	5.832337601129561	0.0
33	1.9556115295033333E-4	0.0	0.0	5.962385767841533	0.0
34	2.7378561413046666E-4	0.0	0.0	6.11339809014978	0.0
35	2.7378561413046666E-4	0.0	0.0	6.337198273586142	0.0
36	3.1289784472053335E-4	0.0	0.0	6.495837480859453	0.0
37	3.1289784472053335E-4	0.0	0.0	6.659639502570651	0.0
38	3.1289784472053335E-4	0.0	0.0	6.804354755753898	0.0
39	3.1289784472053335E-4	0.0	0.0	6.966787849394445	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTCAAG	30	2.1665492E-6	45.000004	1
CGACGTG	30	2.1665492E-6	45.000004	13
GCGAATC	30	2.1665492E-6	45.000004	26
ACGCACC	30	2.1665492E-6	45.000004	45
TGGACGC	30	2.1665492E-6	45.000004	28
ATTACGC	30	2.1665492E-6	45.000004	38
ATTACCG	65	0.0	45.000004	1
CCGTGGC	70	0.0	45.000004	16
AAGCGTG	30	2.1665492E-6	45.000004	3
TAGCCCG	35	1.212702E-7	45.000004	30
ATCGTTC	30	2.1665492E-6	45.000004	20
TCGGAAT	30	2.1665492E-6	45.000004	12
CTTATCG	30	2.1665492E-6	45.000004	22
CGGTGCC	30	2.1665492E-6	45.000004	21
GAACGAT	35	1.212702E-7	45.000004	18
CCGACAT	35	1.212702E-7	45.000004	41
CATCGTA	35	1.212702E-7	45.000004	26
TATAGCG	65	0.0	45.000004	1
TTACGCC	35	1.212702E-7	45.000004	34
ACGTATA	65	0.0	45.000004	27
>>END_MODULE