FastQCFastQC Report
Thu 26 May 2016
SRR1545260_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545260_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences791844
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32700.41296012851016106No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13040.16467890139977068No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC9050.1142901884714666No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG8100.10229287586948944No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGT302.1646429E-645.0000044
AGTAACT351.2112832E-745.00000431
AACGTAG207.031956E-445.01
CGCATCG207.031956E-445.021
TAAGTCG253.889703E-545.01
TCTACGG502.1827873E-1145.02
GTCGTAC207.031956E-445.017
CTACCGT253.889703E-545.039
ATCGGTA253.889703E-545.022
TCGGTAA253.889703E-545.023
GCGATAT900.042.59
TCGAAGG850.042.352942
TAGGGTC1500.042.05
TTGCTAG650.041.5384641
GTTGATC2200.039.88636416
ATTATAC5250.039.85714342
GTATGGC3450.039.7826089
GTACAAC403.4564073E-739.3759
AACAGCG403.4564073E-739.3751
CGGTAGT1150.039.13043212
Produced by FastQC (version 0.11.5)