##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545260_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	791844
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.346811998322902	31.0	31.0	34.0	30.0	34.0
2	31.602578285621917	31.0	31.0	34.0	30.0	34.0
3	31.684835649446104	31.0	31.0	34.0	30.0	34.0
4	35.425550992367185	37.0	35.0	37.0	33.0	37.0
5	35.284342623041915	37.0	35.0	37.0	33.0	37.0
6	35.28110461151439	37.0	35.0	37.0	32.0	37.0
7	35.900109112400926	37.0	35.0	37.0	35.0	37.0
8	35.99031248579266	37.0	35.0	37.0	35.0	37.0
9	37.658804006849834	39.0	37.0	39.0	35.0	39.0
10	36.89957491627138	39.0	37.0	39.0	32.0	39.0
11	36.52517541333899	39.0	35.0	39.0	32.0	39.0
12	35.52706467435505	37.0	35.0	39.0	31.0	39.0
13	35.03903167795677	37.0	34.0	39.0	30.0	39.0
14	35.80882724375003	38.0	34.0	40.0	29.0	41.0
15	36.26933587928936	38.0	35.0	40.0	31.0	41.0
16	36.239484039785616	38.0	35.0	40.0	31.0	41.0
17	36.336813816862914	37.0	35.0	40.0	31.0	41.0
18	36.41303211238577	37.0	35.0	40.0	31.0	41.0
19	36.33956688438632	37.0	35.0	40.0	31.0	41.0
20	36.214880708826485	37.0	35.0	40.0	31.0	41.0
21	36.060677355640756	37.0	34.0	40.0	31.0	41.0
22	35.95189835371614	36.0	34.0	40.0	30.0	41.0
23	35.902152444168294	36.0	34.0	40.0	30.0	41.0
24	35.70772399614065	36.0	34.0	40.0	30.0	41.0
25	35.68967877511227	36.0	34.0	40.0	30.0	41.0
26	35.50942988770515	35.0	34.0	40.0	30.0	41.0
27	35.472705482392996	36.0	34.0	40.0	30.0	41.0
28	35.536699147811944	36.0	34.0	40.0	30.0	41.0
29	35.54600401089103	36.0	34.0	40.0	30.0	41.0
30	35.47871929319411	36.0	34.0	40.0	30.0	41.0
31	35.36251332333136	36.0	34.0	40.0	29.0	41.0
32	35.21678891296771	35.0	34.0	40.0	29.0	41.0
33	35.0519294204414	35.0	34.0	40.0	29.0	41.0
34	34.91463217502437	35.0	34.0	40.0	27.0	41.0
35	34.7510115628836	35.0	34.0	40.0	27.0	41.0
36	34.5290473881219	35.0	33.0	40.0	25.0	41.0
37	34.34199413015695	35.0	33.0	40.0	25.0	41.0
38	34.41796111355267	35.0	33.0	40.0	26.0	41.0
39	34.40996711473472	35.0	33.0	40.0	26.0	41.0
40	34.1903645162431	35.0	33.0	40.0	24.0	41.0
41	34.20786291239183	35.0	33.0	40.0	24.0	41.0
42	34.15839357247135	35.0	33.0	40.0	25.0	41.0
43	34.058957319876136	35.0	33.0	39.0	24.0	41.0
44	34.01239764398038	35.0	33.0	39.0	24.0	41.0
45	33.9386116457282	35.0	33.0	39.0	24.0	41.0
46	33.869681401892294	35.0	33.0	39.0	24.0	41.0
47	33.90614944357727	35.0	33.0	39.0	24.0	41.0
48	33.873466238299464	35.0	33.0	39.0	24.0	41.0
49	33.84606058769152	35.0	33.0	39.0	24.0	41.0
50	33.62996625597971	35.0	33.0	39.0	24.0	41.0
51	33.458195048519656	35.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	0.0
13	3.0
14	6.0
15	21.0
16	71.0
17	169.0
18	370.0
19	771.0
20	1316.0
21	2172.0
22	3385.0
23	4813.0
24	6842.0
25	9594.0
26	12524.0
27	15093.0
28	17194.0
29	19701.0
30	24104.0
31	30068.0
32	38104.0
33	50560.0
34	87030.0
35	127056.0
36	55926.0
37	66382.0
38	89397.0
39	129077.0
40	93.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.831901233071157	24.729239597698538	29.321684574234318	14.117174594995985
2	30.514091159369777	26.069655134092066	30.42228014608938	12.993973560448776
3	28.678249756265124	25.670586630699987	32.359782987558155	13.291380625476734
4	27.99970701299751	27.962578487681917	30.004268517536282	14.03344598178429
5	23.44994721182455	32.74508615333323	28.916427983289637	14.88853865155258
6	22.03843181232667	41.84106970564909	25.83867529462874	10.281823187395497
7	88.06557857355742	4.553043276200868	5.811245649395588	1.5701325008461262
8	89.51952657341597	3.408499653972247	5.61550002273175	1.4564737498800269
9	85.39560822586267	5.025484817716621	7.055682684973302	2.5232242714474062
10	55.425058471113	22.18947166361051	12.142164365708396	10.243305499568097
11	48.73636726425912	20.59723885007653	18.19992321719935	12.466470668465
12	45.82859755204308	19.62949772935073	21.763125059986564	12.778779658619627
13	21.85607266077662	40.761058996469	22.116730062992204	15.266138279762176
14	14.794201888250717	43.61528280822991	25.534827567046037	16.055687736473345
15	13.508721414824132	23.647460863503415	48.15405559680948	14.689762124862979
16	15.54725425715166	17.26640600926445	48.34209768590783	18.844242047676058
17	15.372472355666014	18.230232217456972	29.70686145250832	36.690433974368695
18	19.573173503871974	23.5576704502402	35.91616530528741	20.952990740600423
19	32.14623082324296	23.558175600244493	24.968175549729494	19.327418026783054
20	35.37830683821561	21.73319492223216	24.85439051126232	18.034107728289918
21	23.087502083743768	29.548244351160076	26.770550765049684	20.593702800046472
22	21.689246871858597	26.267168785770934	22.407822752966496	29.635761589403977
23	20.676168538247435	31.684523719318452	24.009021979076685	23.630285763357428
24	19.420239340072033	22.78794813119756	40.81561519693273	16.976197331797678
25	18.184895004571608	24.190623405620297	37.63632735740878	19.98815423239931
26	16.984153444365305	36.22519082041412	26.739357752284544	20.051297982936035
27	17.686438237834725	38.437495264218704	26.489182212658047	17.386884285288517
28	16.12072579952617	29.998711867489053	37.254939104166986	16.625623228817798
29	16.870747268401352	25.67424896823112	36.391511459327845	21.063492304039684
30	18.52372436995166	33.74225226180915	29.154101060309863	18.57992230792934
31	30.207212531761307	27.898171862134465	24.265259318754705	17.62935628734953
32	33.67266784871767	24.497123170725548	25.68536226832558	16.1448467122312
33	31.557099630735348	27.03411277978996	22.528806178994852	18.879981410479843
34	21.083698304211435	26.420481812074097	25.870752319901396	26.625067563813076
35	21.193568430145334	24.571506508857805	31.310712716141058	22.924212344855803
36	32.59568803956335	26.37047196164901	23.350306373477604	17.683533625310037
37	21.292198968483692	36.59167714852926	26.16866453493365	15.947459348053405
38	20.14866564626366	36.01239638110537	23.250918110132805	20.588019862498168
39	21.938538398977574	34.662130419628106	24.57756830890933	18.821762872484985
40	27.35905052005193	24.75209763539283	26.483499275109747	21.405352569445498
41	17.034037007289314	25.038770262829544	27.89337293709367	30.03381979278747
42	19.698576992437903	25.98327448335783	23.93956385348629	30.378584670717967
43	20.68564010082794	25.660483630614113	25.943241345517553	27.710634923040395
44	18.81797424745278	29.864594541349053	30.016518405140406	21.30091280605776
45	17.16626002091321	39.35383737200762	22.658756017599426	20.821146589479746
46	19.694156929900334	36.967887614227045	23.411808386500372	19.92614706937225
47	21.284621718419285	28.40117497890999	25.590394067518353	24.723809235152377
48	21.96038613666328	24.801854910815766	32.554139451710185	20.683619500810764
49	20.784270639166298	27.036512242310355	32.831845666570686	19.347371451952657
50	18.75078929688171	36.938588913978	25.41586474103485	18.894757048105433
51	17.83911982663252	36.38305019675593	23.98161759134375	21.796212385267804
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1507.0
1	1643.0
2	1779.0
3	3818.0
4	5857.0
5	4320.5
6	2784.0
7	2832.5
8	2881.0
9	3168.5
10	3456.0
11	3520.5
12	3585.0
13	3635.0
14	3685.0
15	3693.0
16	3701.0
17	3464.5
18	3228.0
19	3167.0
20	3106.0
21	2935.0
22	2764.0
23	2892.0
24	3020.0
25	3492.0
26	5251.0
27	6538.0
28	8870.0
29	11202.0
30	12861.5
31	14521.0
32	15312.5
33	16104.0
34	18732.5
35	21361.0
36	23122.0
37	24883.0
38	29883.0
39	34883.0
40	47500.5
41	60118.0
42	72688.5
43	85259.0
44	85225.0
45	85191.0
46	76984.5
47	68778.0
48	61684.5
49	54591.0
50	48899.0
51	43207.0
52	38875.0
53	34543.0
54	31097.5
55	27652.0
56	27924.5
57	28197.0
58	29182.5
59	30168.0
60	29286.5
61	28405.0
62	25577.0
63	22749.0
64	18949.0
65	15149.0
66	12481.0
67	9813.0
68	8555.0
69	7297.0
70	6597.5
71	5898.0
72	5049.0
73	4200.0
74	3605.5
75	2187.5
76	1364.0
77	1034.5
78	705.0
79	568.0
80	431.0
81	319.5
82	208.0
83	138.0
84	68.0
85	40.5
86	13.0
87	13.5
88	14.0
89	9.0
90	4.0
91	3.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	791844.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.9489111759479
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.76964587882938	15.663426701726756
2	6.123310786962052	2.565533875575833
3	2.3841886442536815	1.4983846840552204
4	1.5375546730061833	1.2884038509188063
5	1.1402275673868822	1.194326301477747
6	0.9599805951887483	1.2066328931545613
7	0.8644451532047395	1.267642931167533
8	0.7616827790029	1.2765139905264724
9	0.7040794261168979	1.3274727622681737
>10	8.928604897458614	41.12079270731357
>50	1.5663550200037277	22.80786346719973
>100	0.2549736342321358	7.613338568062556
>500	0.003713208265516541	0.5766659279126464
>1k	0.0012377360885055136	0.5930013386403877
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3270	0.41296012851016106	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1304	0.16467890139977068	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	905	0.1142901884714666	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	810	0.10229287586948944	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.013386475113785039	0.0
2	0.0	0.0	0.0	0.057713387990563796	0.0
3	0.0	0.0	0.0	0.08259202570203222	0.0
4	0.0	0.0	0.0	0.11075413844141017	0.0
5	0.0	0.0	0.0	0.1930935891412955	0.0
6	0.0	0.0	0.0	0.27543303984118084	0.0
7	0.0	0.0	0.0	0.3351670278489197	0.0
8	0.0	0.0	0.0	0.4673900414728154	0.0
9	0.0	0.0	0.0	0.527629179484848	0.0
10	0.0	0.0	0.0	0.6249968428124731	0.0
11	0.0	0.0	0.0	0.7605033314642783	0.0
12	0.0	0.0	0.0	0.8633013573380616	0.0
13	0.0	0.0	0.0	0.9062391077030324	0.0
14	0.0	0.0	0.0	0.9221513328382863	0.0
15	0.0	0.0	0.0	0.9472825455519016	0.0
16	0.0	0.0	0.0	1.003480483529584	0.0
17	0.0	0.0	0.0	1.068644834083481	0.0
18	0.0	0.0	0.0	1.1670227974196938	0.0
19	0.0	0.0	0.0	1.2129914478104273	0.0
20	0.0	0.0	0.0	1.2593389607043812	0.0
21	0.0	0.0	0.0	1.3325857113269786	0.0
22	0.0	0.0	0.0	1.4063376119538697	0.0
23	0.0	0.0	0.0	1.4956228752127945	0.0
24	1.2628750107344377E-4	0.0	0.0	1.5653335758053355	0.0
25	1.2628750107344377E-4	0.0	0.0	1.62014235127121	0.0
26	1.2628750107344377E-4	0.0	0.0	1.678234601764994	0.0
27	1.2628750107344377E-4	0.0	0.0	1.7329170897297952	0.0
28	1.2628750107344377E-4	0.0	0.0	1.790883052722506	0.0
29	1.2628750107344377E-4	0.0	0.0	1.8565525532806968	0.0
30	1.2628750107344377E-4	0.0	0.0	1.9405337414945367	0.0
31	1.2628750107344377E-4	0.0	0.0	2.023757204701936	0.0
32	1.2628750107344377E-4	0.0	0.0	2.1049600678921605	0.0
33	2.5257500214688754E-4	0.0	0.0	2.1812377185405207	0.0
34	2.5257500214688754E-4	0.0	0.0	2.270396694298372	0.0
35	2.5257500214688754E-4	0.0	0.0	2.3855708952773527	0.0
36	2.5257500214688754E-4	0.0	0.0	2.480160233581362	0.0
37	2.5257500214688754E-4	0.0	0.0	2.5844737094680266	0.0
38	2.5257500214688754E-4	0.0	0.0	2.6759058602451997	0.0
39	2.5257500214688754E-4	0.0	0.0	2.7756729860932206	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGGGT	30	2.1646429E-6	45.000004	4
AGTAACT	35	1.2112832E-7	45.000004	31
AACGTAG	20	7.031956E-4	45.0	1
CGCATCG	20	7.031956E-4	45.0	21
TAAGTCG	25	3.889703E-5	45.0	1
TCTACGG	50	2.1827873E-11	45.0	2
GTCGTAC	20	7.031956E-4	45.0	17
CTACCGT	25	3.889703E-5	45.0	39
ATCGGTA	25	3.889703E-5	45.0	22
TCGGTAA	25	3.889703E-5	45.0	23
GCGATAT	90	0.0	42.5	9
TCGAAGG	85	0.0	42.35294	2
TAGGGTC	150	0.0	42.0	5
TTGCTAG	65	0.0	41.538464	1
GTTGATC	220	0.0	39.886364	16
ATTATAC	525	0.0	39.857143	42
GTATGGC	345	0.0	39.782608	9
GTACAAC	40	3.4564073E-7	39.375	9
AACAGCG	40	3.4564073E-7	39.375	1
CGGTAGT	115	0.0	39.130432	12
>>END_MODULE