FastQCFastQC Report
Thu 26 May 2016
SRR1545259_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545259_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1793660
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT77860.4340844976193928No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77220.4305163743407335No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70270.3917687856115429No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC37690.21012900995729403No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18460.10291805581882855No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGCG650.045.0000041
TGTTCGG650.045.0000042
GTGCATC253.8916834E-545.010
TTGATCG351.2124292E-745.01
CCTTACC406.8193913E-945.018
TCCGCGA253.8916834E-545.039
ACGCACG253.8916834E-545.01
TCGATTG900.045.01
GCGCATC253.8916834E-545.021
ATCGTAG406.8193913E-945.01
ACGTGCG551.8189894E-1245.01
TATTACG700.045.01
AGCGATG900.045.01
TTACGTA207.034345E-445.032
TTACGCG551.8189894E-1245.01
CTTTCGT207.034345E-445.016
TTGCACG406.8193913E-945.01
GCGCTAA207.034345E-445.041
GTGCGTT207.034345E-445.01
TGTCGAC253.8916834E-545.021