##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545259_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1793660
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.34260506450498	31.0	31.0	34.0	30.0	34.0
2	31.597496738512316	31.0	31.0	34.0	30.0	34.0
3	31.682824504086618	31.0	31.0	34.0	30.0	34.0
4	35.438794977866486	37.0	35.0	37.0	33.0	37.0
5	35.29931257874959	37.0	35.0	37.0	33.0	37.0
6	35.29050488944393	37.0	35.0	37.0	32.0	37.0
7	35.90961218960115	37.0	35.0	37.0	35.0	37.0
8	36.01580232597036	37.0	35.0	37.0	35.0	37.0
9	37.71810320796583	39.0	38.0	39.0	35.0	39.0
10	36.93475575081119	39.0	37.0	39.0	32.0	39.0
11	36.53014562403131	39.0	35.0	39.0	32.0	39.0
12	35.392025244472194	37.0	35.0	39.0	31.0	39.0
13	34.83457734464726	37.0	34.0	39.0	28.0	39.0
14	35.59651940724552	37.0	34.0	40.0	27.0	41.0
15	36.15077718185163	37.0	35.0	40.0	31.0	41.0
16	36.149723470445906	37.0	35.0	40.0	31.0	41.0
17	36.26381365476177	37.0	35.0	40.0	31.0	41.0
18	36.37872952510509	37.0	35.0	40.0	31.0	41.0
19	36.293812093707835	36.0	35.0	40.0	31.0	41.0
20	36.12637512126044	36.0	35.0	40.0	31.0	41.0
21	35.99022724485131	36.0	34.0	40.0	31.0	41.0
22	35.876903092001825	36.0	34.0	40.0	30.0	41.0
23	35.845702084007	35.0	34.0	40.0	31.0	41.0
24	35.663598452326525	35.0	34.0	40.0	30.0	41.0
25	35.676250236945684	35.0	34.0	40.0	30.0	41.0
26	35.56717939854822	35.0	34.0	40.0	30.0	41.0
27	35.536120557965276	35.0	34.0	40.0	30.0	41.0
28	35.65603737609134	36.0	34.0	40.0	30.0	41.0
29	35.672635282048994	36.0	34.0	40.0	30.0	41.0
30	35.59546346576274	36.0	34.0	40.0	30.0	41.0
31	35.44357514802137	36.0	34.0	40.0	30.0	41.0
32	35.30326817791555	35.0	34.0	40.0	30.0	41.0
33	35.11528048794086	35.0	34.0	40.0	29.0	41.0
34	34.99287546134719	35.0	34.0	40.0	29.0	41.0
35	34.86188408059498	35.0	34.0	40.0	28.0	41.0
36	34.56970719088344	35.0	33.0	40.0	26.0	41.0
37	34.406727027418796	35.0	33.0	40.0	26.0	41.0
38	34.5544294905389	35.0	33.0	40.0	27.0	41.0
39	34.583953480592754	35.0	34.0	40.0	27.0	41.0
40	34.42823612055796	35.0	33.0	40.0	25.0	41.0
41	34.496943679404126	35.0	34.0	40.0	27.0	41.0
42	34.424097655073986	35.0	33.0	40.0	27.0	41.0
43	34.3289926741969	35.0	33.0	40.0	27.0	41.0
44	34.261205579652774	35.0	33.0	40.0	26.0	41.0
45	34.20836278893436	35.0	33.0	39.0	26.0	41.0
46	34.14621332917052	35.0	33.0	39.0	26.0	41.0
47	34.20172273452048	35.0	33.0	39.0	26.0	41.0
48	34.27393708952644	35.0	33.0	39.0	27.0	41.0
49	34.29500295485209	35.0	34.0	39.0	26.0	41.0
50	34.09638504510331	35.0	33.0	39.0	26.0	41.0
51	33.89789034711149	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	4.0
14	14.0
15	38.0
16	114.0
17	360.0
18	815.0
19	1526.0
20	2752.0
21	4430.0
22	6965.0
23	10157.0
24	14417.0
25	20535.0
26	26515.0
27	31492.0
28	36043.0
29	42299.0
30	52018.0
31	66291.0
32	86028.0
33	115496.0
34	204443.0
35	317509.0
36	116574.0
37	133918.0
38	191189.0
39	311448.0
40	268.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.29547405862873	25.120201152949832	28.81069990968188	13.77362487873956
2	28.892989752795962	26.781329794944416	31.50139937334835	12.824281078911277
3	26.602421863675392	26.44687398949634	33.01222082222941	13.938483324598867
4	24.69280688647793	29.82772654795223	31.598519228839354	13.880947336730484
5	22.15352965444956	32.94938840136927	30.733583845321853	14.163498098859314
6	20.741166107289008	40.787049942575514	27.826845667517812	10.644938282617664
7	87.89001259993533	4.986954049262402	5.492958531717271	1.6300748190849992
8	89.77972413946902	4.008284736237637	4.839322948607874	1.37266817568547
9	86.46359956736505	4.9546736839757815	6.407903393062231	2.173823355596936
10	55.24976862950615	24.58587469197061	12.346152559570934	7.81820411895231
11	49.24768350746518	17.72309133280555	21.270809406464995	11.758415753264275
12	47.06243100699129	21.279004939620663	21.955387308631515	9.703176744756531
13	18.702262413166377	51.1215057480236	18.700478351527046	11.475753487282986
14	11.602979382937681	49.04641905377831	24.18033518058049	15.170266382703524
15	9.335771550907083	24.19784128541641	52.92301774026292	13.543369423413578
16	10.80271623384588	17.740374429936555	49.641347858568516	21.815561477649055
17	10.606023438109787	19.52449182119242	29.624622280699796	40.244862459998
18	16.868971822976484	22.90328155837784	40.95491899245119	19.272827626194484
19	29.268478975948618	25.998015231426248	24.229564131440743	20.503941661184395
20	32.37938070760345	21.884192098837016	27.324130548710457	18.41229664484908
21	17.070347780515817	33.975335347836264	26.98554910072143	21.968767770926487
22	19.846236187460274	26.7745838118707	20.66333641827325	32.71584358239577
23	14.573051748937926	34.261621489022446	24.9631479767626	26.20217878527703
24	13.74134451345294	23.147586499113544	49.26262502369457	13.848443963738946
25	11.553917687856115	29.931480882664495	40.64064538429803	17.87395604518136
26	13.070648840917453	39.9541161647135	26.75278480871514	20.222450185653916
27	11.947136023549614	43.47122643087319	25.623752550650625	18.957884994926573
28	9.975413400532988	32.0054525383852	40.00903181204911	18.010102249032702
29	10.4283420492178	23.5079111983319	40.7668677452806	25.296879007169697
30	14.846291939386505	39.00588740340979	30.323026660571124	15.824793996632582
31	29.009678534393363	32.119130715966236	23.376002140873968	15.495188608766433
32	32.19556660682626	25.23019970339975	24.98009656233623	17.594137127437754
33	29.817969960862143	30.11635427004003	21.770736928960897	18.294938840136926
34	16.132042862080883	26.056108738556915	27.004114492155708	30.80773390720649
35	17.39872662600493	25.349230065898777	33.56516842656914	23.686874881527157
36	31.25079446494876	28.362008407390476	23.60965846369992	16.77753866396084
37	15.547260907864366	40.41195098290646	28.647904285093052	15.392883824136124
38	15.082066835409163	38.283398191407514	27.64481562837996	18.989719344803362
39	16.51483558756955	40.11875160286788	26.57064326572483	16.79576954383774
40	25.554118394790542	26.220130905522787	25.6037376091344	22.62201309055228
41	13.885184483123892	24.343409564800464	28.495534270709054	33.275871681366596
42	15.860363725566717	24.80720983909994	25.36545387643143	33.96697255890191
43	17.349943690554507	25.32486647413668	28.526811101323553	28.79837873398526
44	15.415742113890035	30.03462194618824	32.782913149649325	21.766722790272404
45	12.738144352887392	46.23674497953904	20.971477314541218	20.053633353032346
46	17.50259246456965	40.190281324219754	24.69676527324019	17.610360937970405
47	15.60050399741311	30.33194696876777	28.49386171292218	25.573687320896937
48	17.038569182565258	24.708696185453206	37.339852591907054	20.912882040074486
49	17.675367683953482	27.250872517645487	36.86138956100933	18.2123702373917
50	16.678913506461647	39.7973417481574	27.520767592520322	16.00297715286063
51	13.957884994926575	36.89439470133693	26.57945207006902	22.568268233667474
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7986.0
1	6220.5
2	4455.0
3	8280.0
4	12105.0
5	8582.0
6	5059.0
7	5498.0
8	5937.0
9	6508.0
10	7079.0
11	7469.0
12	7859.0
13	7761.0
14	7663.0
15	7552.0
16	7441.0
17	6958.0
18	6475.0
19	6348.5
20	6222.0
21	5914.0
22	5606.0
23	6195.5
24	6785.0
25	8146.5
26	12775.5
27	16043.0
28	20836.5
29	25630.0
30	32184.5
31	38739.0
32	46383.5
33	54028.0
34	66527.0
35	79026.0
36	81729.0
37	84432.0
38	95203.0
39	105974.0
40	139555.0
41	173136.0
42	204463.5
43	235791.0
44	229364.5
45	222938.0
46	205527.0
47	188116.0
48	160787.5
49	133459.0
50	117417.0
51	101375.0
52	86321.0
53	71267.0
54	58375.5
55	45484.0
56	38862.5
57	32241.0
58	28237.0
59	24233.0
60	21051.0
61	17869.0
62	15821.5
63	13774.0
64	11378.0
65	8982.0
66	7748.5
67	6515.0
68	5316.5
69	4118.0
70	3904.0
71	3690.0
72	3253.0
73	2816.0
74	2400.5
75	1381.0
76	777.0
77	682.5
78	588.0
79	403.5
80	219.0
81	185.5
82	152.0
83	89.0
84	26.0
85	33.0
86	40.0
87	23.0
88	6.0
89	8.5
90	11.0
91	5.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1793660.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.319759883107015
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.33931247985296	12.78478769078889
2	8.40510536924007	2.7433860283642293
3	2.9984364785825948	1.4680129006565068
4	1.4821898907305437	0.9675593247156439
5	0.9049491019341306	0.7384276024999172
6	0.5647212841592765	0.5529669455015542
7	0.42867384845485706	0.48970979924654706
8	0.3493411822102672	0.4560931368761837
9	0.27927545539794457	0.4101937536005836
>10	3.4030607436130116	13.362376733433448
>50	1.317746682099644	15.126284682285466
>100	1.5003451505232843	46.56155865480635
>500	0.022957258659136833	2.257740413550732
>1k	0.00282550875804761	0.7819955116324427
>5k	0.001059565784267854	1.2989068220415048
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	7786	0.4340844976193928	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7722	0.4305163743407335	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7027	0.3917687856115429	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3769	0.21012900995729403	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1846	0.10291805581882855	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0390821002865649	0.0
2	0.0	0.0	0.0	0.12967898040877313	0.0
3	0.0	0.0	0.0	0.18927778954762886	0.0
4	0.0	0.0	0.0	0.2535597604897249	0.0
5	0.0	0.0	0.0	0.45499146995528694	0.0
6	0.0	0.0	0.0	0.6537470869618546	0.0
7	0.0	0.0	0.0	0.801322435690153	0.0
8	0.0	0.0	0.0	1.1814390687198242	0.0
9	0.0	0.0	0.0	1.3483045839233745	0.0
10	0.0	0.0	0.0	1.624276618757178	0.0
11	0.0	0.0	0.0	1.8587692204765676	0.0
12	0.0	0.0	0.0	2.0724106017862916	0.0
13	0.0	0.0	0.0	2.158491575883947	0.0
14	0.0	0.0	0.0	2.18592152358864	0.0
15	0.0	0.0	0.0	2.2302443049407357	0.0
16	0.0	0.0	0.0	2.3399640957595085	0.0
17	0.0	0.0	0.0	2.473043943668254	0.0
18	0.0	0.0	0.0	2.697947214076246	0.0
19	0.0	0.0	0.0	2.780125553337868	0.0
20	0.0	0.0	0.0	2.86754457366502	0.0
21	0.0	0.0	0.0	2.997892577188542	0.0
22	0.0	0.0	0.0	3.1283520845645216	0.0
23	0.0	0.0	0.0	3.303747644481117	0.0
24	1.6725577868715365E-4	0.0	0.0	3.4331478652587446	0.0
25	2.2300770491620486E-4	0.0	0.0	3.5289296745202545	0.0
26	2.2300770491620486E-4	0.0	0.0	3.628056599355508	0.0
27	2.2300770491620486E-4	0.0	0.0	3.723057881649811	0.0
28	2.2300770491620486E-4	0.0	0.0	3.824637891239142	0.0
29	2.2300770491620486E-4	0.0	0.0	3.9442815249266863	0.0
30	2.2300770491620486E-4	0.0	0.0	4.121015131072778	0.0
31	2.2300770491620486E-4	0.0	0.0	4.255767536768396	0.0
32	2.2300770491620486E-4	0.0	0.0	4.37184304717728	0.0
33	2.2300770491620486E-4	0.0	0.0	4.480392047545243	0.0
34	2.2300770491620486E-4	0.0	0.0	4.610461291437619	0.0
35	2.2300770491620486E-4	0.0	0.0	4.813286799058908	0.0
36	2.2300770491620486E-4	0.0	0.0	4.945418864221759	0.0
37	2.2300770491620486E-4	0.0	0.0	5.080673037253437	0.0
38	2.7875963114525607E-4	0.0	0.0	5.21035201766221	0.0
39	2.7875963114525607E-4	0.0	0.0	5.338023928726738	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTAGCG	65	0.0	45.000004	1
TGTTCGG	65	0.0	45.000004	2
GTGCATC	25	3.8916834E-5	45.0	10
TTGATCG	35	1.2124292E-7	45.0	1
CCTTACC	40	6.8193913E-9	45.0	18
TCCGCGA	25	3.8916834E-5	45.0	39
ACGCACG	25	3.8916834E-5	45.0	1
TCGATTG	90	0.0	45.0	1
GCGCATC	25	3.8916834E-5	45.0	21
ATCGTAG	40	6.8193913E-9	45.0	1
ACGTGCG	55	1.8189894E-12	45.0	1
TATTACG	70	0.0	45.0	1
AGCGATG	90	0.0	45.0	1
TTACGTA	20	7.034345E-4	45.0	32
TTACGCG	55	1.8189894E-12	45.0	1
CTTTCGT	20	7.034345E-4	45.0	16
TTGCACG	40	6.8193913E-9	45.0	1
GCGCTAA	20	7.034345E-4	45.0	41
GTGCGTT	20	7.034345E-4	45.0	1
TGTCGAC	25	3.8916834E-5	45.0	21
>>END_MODULE