FastQCFastQC Report
Thu 26 May 2016
SRR1545258_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545258_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3605404
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT590651.6382352712761177No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA98880.27425497946970717No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT362050.042.880821
ACGGGTA2700.040.8333325
AGGGTAC15450.039.320396
CATATGC24850.038.5714333
GCGCGAC21450.037.9720279
ATACTAT34900.037.9083145
GCTACGA4350.037.7586210
TAGCATA25500.037.58823430
GTTTTTT430950.037.4399572
GTTGATC26100.037.06896616
ACCCGCT21500.037.0465134
CCAGTAG26300.036.9581726
GGTACCT26500.036.8490528
TAAGGGA31050.036.8115924
GCCCTAC37750.036.7152326
TATACTA36150.036.6597544
TAGGGTA17100.036.5789455
TTGATCC26550.036.52542517
CCCTACA37900.036.45118727
TAGCCGT5000.036.4544
Produced by FastQC (version 0.11.5)