##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545258_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3605404
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.13957548169359	31.0	31.0	33.0	30.0	34.0
2	31.437143243863932	31.0	31.0	34.0	30.0	34.0
3	31.46891721427058	31.0	31.0	34.0	30.0	34.0
4	35.28719250325345	37.0	35.0	37.0	33.0	37.0
5	35.170893469913494	37.0	35.0	37.0	32.0	37.0
6	35.163718407146604	37.0	35.0	37.0	32.0	37.0
7	35.7937640275542	37.0	35.0	37.0	35.0	37.0
8	35.865308020959645	37.0	35.0	37.0	35.0	37.0
9	37.484116620495236	39.0	37.0	39.0	35.0	39.0
10	36.75737892341607	39.0	37.0	39.0	32.0	39.0
11	36.45637687205095	38.0	35.0	39.0	32.0	39.0
12	35.96970186974885	38.0	35.0	39.0	31.0	39.0
13	35.768634804865144	38.0	35.0	39.0	30.0	39.0
14	36.60809634648433	39.0	35.0	41.0	30.0	41.0
15	36.867928809087694	39.0	35.0	41.0	31.0	41.0
16	36.695909251778716	38.0	35.0	41.0	31.0	41.0
17	36.74713957159863	38.0	35.0	41.0	31.0	41.0
18	36.66275845924618	38.0	35.0	40.0	31.0	41.0
19	36.492918408034164	38.0	35.0	40.0	31.0	41.0
20	36.290332234612265	38.0	35.0	40.0	31.0	41.0
21	36.15158273524964	38.0	35.0	40.0	30.0	41.0
22	36.09928429657259	37.0	35.0	40.0	30.0	41.0
23	35.99900538192114	37.0	34.0	40.0	30.0	41.0
24	35.733394371338136	37.0	34.0	40.0	30.0	41.0
25	35.67203425746463	37.0	34.0	40.0	29.0	41.0
26	35.542266275845925	37.0	34.0	40.0	29.0	41.0
27	35.52016140216187	37.0	34.0	40.0	29.0	41.0
28	35.450766959819205	37.0	34.0	40.0	29.0	41.0
29	35.33490615753463	37.0	34.0	40.0	28.0	41.0
30	35.16982729258635	36.0	34.0	40.0	27.0	41.0
31	34.96972489074734	36.0	34.0	40.0	26.0	41.0
32	34.69153997721199	36.0	33.0	40.0	25.0	41.0
33	34.331170376468215	36.0	33.0	40.0	23.0	41.0
34	33.92308240629899	36.0	33.0	40.0	21.0	41.0
35	33.64443956904691	36.0	33.0	40.0	21.0	41.0
36	33.43792207475223	36.0	32.0	40.0	19.0	41.0
37	33.233062924432325	35.0	32.0	40.0	18.0	41.0
38	33.190812458187764	35.0	32.0	40.0	18.0	41.0
39	33.126414404599316	35.0	32.0	40.0	18.0	41.0
40	32.98859517546438	35.0	32.0	40.0	18.0	41.0
41	32.871246606482934	35.0	31.0	40.0	18.0	41.0
42	32.76175540937992	35.0	31.0	40.0	18.0	41.0
43	32.59966788742676	35.0	31.0	39.0	17.0	41.0
44	32.500660397558775	35.0	31.0	39.0	17.0	41.0
45	32.439823387337455	35.0	31.0	39.0	17.0	41.0
46	32.34121002805788	35.0	31.0	39.0	17.0	41.0
47	32.33522512317621	35.0	31.0	39.0	17.0	41.0
48	32.26390301891272	35.0	31.0	39.0	17.0	41.0
49	32.19240423541994	35.0	31.0	39.0	17.0	41.0
50	32.02569920042248	35.0	30.0	39.0	15.0	40.0
51	31.85920606955559	35.0	30.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	6.0
13	21.0
14	64.0
15	181.0
16	557.0
17	1552.0
18	3341.0
19	6508.0
20	10987.0
21	16981.0
22	24759.0
23	35569.0
24	49877.0
25	70736.0
26	91635.0
27	103273.0
28	104863.0
29	108332.0
30	120208.0
31	139682.0
32	166926.0
33	208490.0
34	306457.0
35	385289.0
36	302186.0
37	357183.0
38	449126.0
39	540373.0
40	238.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.40532822396602	21.44181345557946	25.006878563400942	21.145979757053578
2	38.41358693782999	23.26280217140714	26.37765421018005	11.945956680582814
3	28.01636099588285	24.122705804952787	35.592321970020556	12.268611229143806
4	24.75334248256229	26.36692032293746	34.945986635616975	13.933750558883276
5	21.390168757786924	30.48127200169523	34.1715935301564	13.956965710361446
6	19.92320416796564	38.53202026735423	31.81624028818962	9.728535276490513
7	80.52343093866872	4.321485192782834	13.31404192151559	1.841041947032843
8	81.10724900732346	3.5785726093386483	13.371067431000796	1.9431109523371028
9	76.89052322569121	5.059349798247298	14.854895595611476	3.1952313804500134
10	44.00413934194337	22.87427428382506	20.980034415005917	12.141551959225652
11	33.544784440245806	23.229962578396208	27.436065417356836	15.78918756400115
12	29.588584247424144	20.89527276277499	32.76464995323686	16.751493036564003
13	22.86023979559572	27.05277966075369	32.780903332885856	17.30607721076473
14	18.855584561397283	29.300377988153336	34.75996032622142	17.084077124227964
15	17.32618591425538	22.30296521554866	43.157188487059976	17.21366038313598
16	20.806212008418473	19.772291815286163	39.65228307285397	19.76921310344139
17	20.26483023816471	20.03237362581281	33.96354472342073	25.73925141260175
18	22.35352820377411	21.851448547791037	36.657861365882994	19.137161882551858
19	27.832830939334396	23.04091302944136	30.39440240261563	18.73185362860861
20	28.09818261698273	23.076248875299413	30.60087024921479	18.224698258503068
21	23.53655790030743	24.173241057035494	33.37273714679409	18.917463895862987
22	23.158070496399294	21.70489076952264	31.428017498177734	23.709021235900334
23	21.479007623001472	25.266239234216194	31.202134351656568	22.052618791125767
24	20.58387908816876	23.20156076822459	37.043366013905796	19.17119412970086
25	21.961172728493118	23.89066523474207	34.34186571047239	19.806296326292422
26	19.732018936019376	26.63049688745006	31.678086561173174	21.95939761535739
27	19.34984262512606	26.42583189012937	33.18868010353347	21.035645381211094
28	19.757036936776018	24.316858804172846	36.04661225205275	19.879492006998383
29	20.16140216186591	23.926112025171104	35.33731587361638	20.5751699393466
30	21.407781208430457	25.187773686388542	33.930122671412136	19.474322433768865
31	25.78030090386542	24.48194432579539	30.44258008256495	19.295174687774242
32	26.88586355370993	23.58842448724193	29.96044271321605	19.56526924583209
33	26.162615895472463	24.718533623416405	28.543236763480596	20.575613717630535
34	21.50358184547418	25.46985025811254	30.505346973598517	22.521220922814752
35	22.41390978653155	25.752647969547933	30.938751940143185	20.89469030377733
36	27.247792480398868	26.074525906112044	27.28604062124522	19.391640992243865
37	22.752873187027028	29.562650953956894	28.30614821529016	19.378327643725918
38	22.588314652116654	29.379980717833565	27.827422391498985	20.204282238550796
39	22.209494414495573	27.91537370014567	27.958697555114487	21.916434330244268
40	24.358268865292214	24.42913471000753	30.047367784581148	21.16522864011911
41	20.946806515996546	24.745825987878195	29.9815221817028	24.325845314422462
42	22.219867731882477	26.001774003690016	27.59136008059014	24.186998183837375
43	21.65235851516224	25.11563197910692	29.383863777817965	23.84814572791288
44	21.33475194458097	26.1539622189358	30.82234334903939	21.68894248744385
45	20.216042363074983	29.859788251191823	28.129912764283837	21.794256621449357
46	21.668528686382995	28.576436926347228	28.54903361731445	21.20600076995532
47	21.277976060380475	27.40167260035214	29.12383743957681	22.196513899690576
48	21.783911040205204	26.103676592137802	30.52534473251819	21.58706763513881
49	22.030429876929187	26.264657164633974	30.624335025977672	21.080577932459164
50	20.615026776472206	29.00790036289969	29.184856953617405	21.1922159070107
51	20.56191206311415	29.00731790390203	29.195424424003523	21.235345608980296
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	11128.0
1	9740.0
2	8352.0
3	50303.5
4	92255.0
5	65084.0
6	37913.0
7	37250.0
8	36587.0
9	37826.5
10	39066.0
11	39060.0
12	39054.0
13	38414.0
14	37774.0
15	36053.5
16	34333.0
17	32133.0
18	29933.0
19	27777.5
20	25622.0
21	25877.5
22	26133.0
23	25055.0
24	23977.0
25	25907.0
26	30256.0
27	32675.0
28	36530.0
29	40385.0
30	48111.5
31	55838.0
32	64366.0
33	72894.0
34	84379.0
35	95864.0
36	101883.0
37	107902.0
38	119622.5
39	131343.0
40	154904.0
41	178465.0
42	199050.5
43	219636.0
44	228280.0
45	236924.0
46	234181.5
47	231439.0
48	225325.5
49	219212.0
50	203428.0
51	187644.0
52	179106.5
53	170569.0
54	163937.0
55	157305.0
56	158752.5
57	160200.0
58	159905.5
59	159611.0
60	152510.0
61	145409.0
62	136603.5
63	127798.0
64	119673.0
65	111548.0
66	97644.5
67	83741.0
68	75598.5
69	67456.0
70	59508.5
71	51561.0
72	46139.0
73	40717.0
74	31709.5
75	17970.0
76	13238.0
77	9937.5
78	6637.0
79	5101.0
80	3565.0
81	2640.0
82	1715.0
83	1283.5
84	852.0
85	586.5
86	321.0
87	258.5
88	196.0
89	117.5
90	39.0
91	29.0
92	19.0
93	17.5
94	16.0
95	9.0
96	2.0
97	1.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3605404.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.31979992983453
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.80858892751353	18.378005265421884
2	5.8896844949822	2.7469252814566687
3	2.414553760457851	1.6892073184112006
4	1.4985833363107863	1.3978665432380588
5	1.1220063208616458	1.3082481461251656
6	0.8441379086513573	1.1811076285763153
7	0.6277021866108101	1.0246522585098625
8	0.5429465230039318	1.012912343124079
9	0.46517573899991643	0.976302464912188
>10	6.098429195360834	34.02907585271752
>50	1.3208635287526387	21.242854436421794
>100	0.3610997862487238	11.614110322882572
>500	0.003907948075745579	0.6541609593816117
>1k	0.0020760974152396736	0.7808637068038042
>5k	1.2212337736703963E-4	0.281599632841315
>10k+	1.2212337736703963E-4	1.6821078391759983
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	59065	1.6382352712761177	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9888	0.27425497946970717	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.7736142745722808E-5	0.0	0.0	0.017834339785499767	0.0
2	5.5472285491445616E-5	0.0	0.0	0.06687184015993769	0.0
3	5.5472285491445616E-5	0.0	0.0	0.1324400816108264	0.0
4	5.5472285491445616E-5	0.0	0.0	0.17357278130273335	0.0
5	5.5472285491445616E-5	0.0	0.0	0.29009231697751486	0.0
6	5.5472285491445616E-5	0.0	0.0	0.5206351354799629	0.0
7	5.5472285491445616E-5	0.0	0.0	0.6803398454098348	0.0
8	5.5472285491445616E-5	0.0	0.0	1.009623332087056	0.0
9	5.5472285491445616E-5	0.0	0.0	1.2563640579530062	0.0
10	5.5472285491445616E-5	0.0	0.0	1.5812097617909115	0.0
11	5.5472285491445616E-5	0.0	0.0	1.8122795670055283	0.0
12	5.5472285491445616E-5	0.0	0.0	1.9689610373761166	0.0
13	8.320842823716843E-5	0.0	0.0	2.058188208589107	0.0
14	1.1094457098289123E-4	0.0	0.0	2.1214820863348463	0.0
15	1.1094457098289123E-4	0.0	0.0	2.1639461208785478	0.0
16	1.1094457098289123E-4	0.0	0.0	2.2242167590650035	0.0
17	1.1094457098289123E-4	0.0	0.0	2.2891470692327407	0.0
18	1.1094457098289123E-4	0.0	0.0	2.4023105316352895	0.0
19	1.3868071372861405E-4	0.0	0.0	2.459613402547953	0.0
20	1.3868071372861405E-4	0.0	0.0	2.5255699499972817	0.0
21	1.3868071372861405E-4	0.0	0.0	2.597933546420873	0.0
22	1.3868071372861405E-4	0.0	0.0	2.681585752941973	0.0
23	1.3868071372861405E-4	0.0	0.0	2.774640511853873	0.0
24	1.3868071372861405E-4	0.0	0.0	2.8571278003796525	0.0
25	1.6641685647433686E-4	0.0	0.0	2.9257192813898247	0.0
26	1.6641685647433686E-4	0.0	0.0	2.9908992168422732	0.0
27	1.6641685647433686E-4	0.0	0.0	3.073913492080222	0.0
28	1.6641685647433686E-4	0.0	0.0	3.1483572992097417	0.0
29	1.6641685647433686E-4	0.0	0.0	3.2397756256996444	0.0
30	1.6641685647433686E-4	0.0	0.0	3.367278673901732	0.0
31	1.6641685647433686E-4	0.0	0.0	3.455756969260588	0.0
32	1.6641685647433686E-4	0.0	0.0	3.549366451027402	0.0
33	1.6641685647433686E-4	0.0	0.0	3.643031405079708	0.0
34	1.6641685647433686E-4	0.0	0.0	3.7427705743933273	0.0
35	1.6641685647433686E-4	0.0	0.0	3.8616199460587497	0.0
36	1.9415299922005967E-4	0.0	0.0	3.9603883503762685	0.0
37	1.9415299922005967E-4	0.0	0.0	4.069585544366179	0.0
38	1.9415299922005967E-4	0.0	0.0	4.2042722535394095	0.0
39	1.9415299922005967E-4	0.0	0.0	4.44374611000598	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	36205	0.0	42.88082	1
ACGGGTA	270	0.0	40.833332	5
AGGGTAC	1545	0.0	39.32039	6
CATATGC	2485	0.0	38.57143	33
GCGCGAC	2145	0.0	37.972027	9
ATACTAT	3490	0.0	37.90831	45
GCTACGA	435	0.0	37.75862	10
TAGCATA	2550	0.0	37.588234	30
GTTTTTT	43095	0.0	37.439957	2
GTTGATC	2610	0.0	37.068966	16
ACCCGCT	2150	0.0	37.04651	34
CCAGTAG	2630	0.0	36.95817	26
GGTACCT	2650	0.0	36.849052	8
TAAGGGA	3105	0.0	36.811592	4
GCCCTAC	3775	0.0	36.71523	26
TATACTA	3615	0.0	36.65975	44
TAGGGTA	1710	0.0	36.578945	5
TTGATCC	2655	0.0	36.525425	17
CCCTACA	3790	0.0	36.451187	27
TAGCCGT	500	0.0	36.45	44
>>END_MODULE