FastQCFastQC Report
Thu 26 May 2016
SRR1545257_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545257_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4708582
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT671021.4250999557828663No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA89970.19107663411192585No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT436750.042.5684051
TACGGGA8300.039.3072284
ACGTTAG2200.038.8636321
GGGCGAT76900.037.7730837
CGTAAGG5400.037.52
TAGGGAC30400.037.1546065
GTTTTTT524450.036.98162
ACGGGCT16550.036.978855
AGGGCGA40600.036.6871956
CGGTCTA4550.036.59340731
TCGTAAG1600.036.56251
ACGGGAT10350.036.521745
ATAGGGC23350.036.520344
TACGGGC7800.036.3461574
CATATGC30900.036.3349533
TCAGACG30300.036.31188222
GGTACCT32550.036.0829478
ACCCGCT30250.036.0743834
GCGTAAG2750.036.01
GTAGCAT31100.035.9565929
Produced by FastQC (version 0.11.5)