##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545257_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4708582
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.120632708530934	31.0	31.0	33.0	30.0	34.0
2	31.420778909659003	31.0	31.0	34.0	30.0	34.0
3	31.469321549460112	31.0	31.0	34.0	30.0	34.0
4	35.285209644856984	37.0	35.0	37.0	33.0	37.0
5	35.16217154124108	37.0	35.0	37.0	32.0	37.0
6	35.1551789901928	37.0	35.0	37.0	32.0	37.0
7	35.78957465326079	37.0	35.0	37.0	35.0	37.0
8	35.8538804251471	37.0	35.0	37.0	35.0	37.0
9	37.45551080983617	39.0	37.0	39.0	35.0	39.0
10	36.705126511548485	39.0	37.0	39.0	32.0	39.0
11	36.454334447186014	38.0	35.0	39.0	32.0	39.0
12	35.98724159417846	38.0	35.0	39.0	31.0	39.0
13	35.785753120578555	38.0	35.0	39.0	30.0	39.0
14	36.63364087107329	39.0	35.0	41.0	31.0	41.0
15	36.89216774816707	39.0	35.0	41.0	31.0	41.0
16	36.690642533144796	38.0	35.0	41.0	31.0	41.0
17	36.72423162642171	38.0	35.0	41.0	31.0	41.0
18	36.65264446918414	38.0	35.0	40.0	31.0	41.0
19	36.511376461108675	38.0	35.0	40.0	31.0	41.0
20	36.33975366681519	38.0	35.0	40.0	31.0	41.0
21	36.24570539495755	38.0	35.0	40.0	30.0	41.0
22	36.171063177831456	38.0	35.0	40.0	30.0	41.0
23	36.06397934664831	38.0	34.0	40.0	30.0	41.0
24	35.823281191662375	37.0	34.0	40.0	30.0	41.0
25	35.76040621146664	37.0	34.0	40.0	30.0	41.0
26	35.628719219501754	37.0	34.0	40.0	29.0	41.0
27	35.606013232858636	37.0	34.0	40.0	29.0	41.0
28	35.54021847766482	37.0	34.0	40.0	29.0	41.0
29	35.420776361121035	37.0	34.0	40.0	29.0	41.0
30	35.24886175923027	37.0	34.0	40.0	27.0	41.0
31	35.0695447164348	37.0	34.0	40.0	27.0	41.0
32	34.80841748110153	37.0	33.0	40.0	25.0	41.0
33	34.47028744534979	37.0	33.0	40.0	23.0	41.0
34	34.10766277405809	36.0	33.0	40.0	22.0	41.0
35	33.84786162798057	36.0	33.0	40.0	21.0	41.0
36	33.67610822111625	36.0	33.0	40.0	20.0	41.0
37	33.492750683751495	36.0	32.0	40.0	19.0	41.0
38	33.458483254618905	36.0	32.0	40.0	19.0	41.0
39	33.40118914781563	36.0	32.0	40.0	19.0	41.0
40	33.2647196119766	36.0	32.0	40.0	18.0	41.0
41	33.15075281687778	35.0	32.0	40.0	18.0	41.0
42	33.04773581515624	35.0	32.0	40.0	18.0	41.0
43	32.88221613216038	35.0	31.0	40.0	18.0	41.0
44	32.79569454243337	35.0	31.0	39.0	18.0	41.0
45	32.749978655994525	35.0	31.0	39.0	18.0	41.0
46	32.65819858292794	35.0	31.0	39.0	18.0	41.0
47	32.65997746242924	35.0	31.0	39.0	18.0	41.0
48	32.59078147093966	35.0	31.0	39.0	18.0	41.0
49	32.550208746497354	35.0	31.0	39.0	18.0	41.0
50	32.384436758242714	35.0	31.0	39.0	18.0	41.0
51	32.20851946509586	35.0	31.0	39.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	5.0
12	13.0
13	22.0
14	70.0
15	246.0
16	729.0
17	1874.0
18	4175.0
19	8089.0
20	13873.0
21	21223.0
22	30916.0
23	44336.0
24	62380.0
25	87992.0
26	115041.0
27	128543.0
28	131672.0
29	137285.0
30	154441.0
31	178938.0
32	215119.0
33	268112.0
34	394899.0
35	500988.0
36	386159.0
37	463405.0
38	603679.0
39	754045.0
40	308.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.49490356119953	21.72367392136316	24.662669143279228	20.11875337415808
2	38.3355753388175	23.267026038837173	26.307410596226212	12.089988026119117
3	28.610184552376914	24.154087154051897	34.440071342072834	12.79565695149835
4	25.456644909231695	26.178051056560125	33.593956736868975	14.771347297339199
5	21.644180774594133	30.204995898977653	33.46771915621306	14.683104170215152
6	20.43555788133243	38.548399496918606	31.50848811807886	9.507554503670107
7	80.93230615926409	4.342071562096614	12.776925197437361	1.9486970812019417
8	81.45792087724075	3.725389087415277	12.726995091091117	2.0896949442528556
9	76.77430275186882	5.2189810010742095	14.494894641316641	3.511821605740327
10	41.57306807017484	23.740544393195233	21.311957612716526	13.3744299239134
11	33.3913054928214	23.916690842380998	27.238879985524306	15.453123679273292
12	30.392313439587543	21.169558053783497	32.33444803552322	16.10368047110574
13	22.89001232218107	27.383382088280506	32.34449352267838	17.382112066860046
14	19.024198792757566	29.599314613189275	34.18623696051168	17.190249633541477
15	18.26944927368792	22.65478226778253	41.918692294198124	17.15707616433143
16	21.462767346942243	20.05210910630844	39.292848675036346	19.192274871712968
17	20.834255408528513	20.22674767053011	33.65594992292797	25.283046998013415
18	22.387185781196973	22.308520909267376	36.351283677336404	18.953009632199247
19	27.441955985899792	24.000304125530786	30.090120550093424	18.467619338476
20	27.958332253744334	23.705098477630845	29.938333876313507	18.398235392311317
21	23.830656448162102	25.391954520490458	32.418804642246855	18.35858438910058
22	22.704542471597605	22.556217561890183	31.312548024012326	23.426691942499886
23	21.21662105491632	25.67673240054012	31.5048564514752	21.60179009306836
24	20.940911722467614	23.762270679368015	37.044677144838936	18.25214045332544
25	22.1068890804068	23.78234041586193	34.305105018878294	19.805665484852977
26	19.26212604983836	27.956144758655576	31.280436445622055	21.501292745884005
27	19.510757166382575	26.80167829720285	33.27328694711061	20.414277589303957
28	19.781454374161903	25.48529472354947	35.7786909094925	18.95455999279613
29	20.47210816334939	24.566206981210055	35.09782775366342	19.86385710177714
30	21.261326658429226	26.30545671711781	33.60147492387305	18.831741700579922
31	25.89138301085125	24.798697357293555	30.54698845639728	18.76293117545792
32	27.106695816277597	24.18277944400246	30.025515112617768	18.685009627102172
33	26.59503434367289	24.79793279590331	28.480485207648503	20.126547652775294
34	21.562521370552748	25.880403059774682	30.403081012500156	22.153994557172414
35	21.869110488040775	26.021910630419093	30.902339600329782	21.20663928121035
36	26.073221194831053	27.431315839885556	27.03546418008649	19.459998785196902
37	22.15265657473949	30.529828300749568	27.719003300781424	19.598511823729524
38	22.5394184491212	30.461654910119435	26.713690023875554	20.285236616883807
39	22.18081791928016	28.91044480057903	26.83839848175098	22.070338798389834
40	24.19896266009597	25.265462086037793	29.151706394833944	21.383868859032297
41	20.37949004604783	25.30853237768823	29.626371591277373	24.685605984986562
42	21.55400500617808	26.885758812313348	27.441382564857104	24.118853616651467
43	21.289169435723963	25.532124108701943	28.86380655577412	24.314899899799983
44	21.058080755522575	26.729852002152665	30.686881952995616	21.525185289329144
45	20.577384019222773	30.012241477370473	27.910589642486844	21.499784860919913
46	21.992034969338963	29.398171254105797	28.125155301532395	20.48463847502284
47	21.082950238521917	27.741303007996887	28.744980973040292	22.430765780440908
48	21.129015062284147	26.79726083139255	30.682676865349272	21.391047240974036
49	22.06112158607411	26.312762525957922	31.163479790731053	20.462636097236917
50	20.314183760631117	29.327279422977025	29.258808702917356	21.0997281134745
51	19.95044367922232	29.67628045980722	29.12573254538203	21.24754331558843
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	10504.0
1	10428.0
2	10352.0
3	59380.0
4	108408.0
5	76886.0
6	45364.0
7	44756.0
8	44148.0
9	44988.0
10	45828.0
11	45959.5
12	46091.0
13	45267.5
14	44444.0
15	42847.5
16	41251.0
17	39439.5
18	37628.0
19	35668.0
20	33708.0
21	33301.0
22	32894.0
23	32373.0
24	31852.0
25	34651.0
26	41670.0
27	45890.0
28	51733.0
29	57576.0
30	66905.0
31	76234.0
32	90169.5
33	104105.0
34	114918.5
35	125732.0
36	137867.0
37	150002.0
38	162476.5
39	174951.0
40	209203.0
41	243455.0
42	270515.0
43	297575.0
44	311975.5
45	326376.0
46	326023.5
47	325671.0
48	314292.0
49	302913.0
50	281947.0
51	260981.0
52	243105.0
53	225229.0
54	217469.5
55	209710.0
56	211882.5
57	214055.0
58	209869.5
59	205684.0
60	198494.5
61	191305.0
62	176337.0
63	161369.0
64	142515.0
65	123661.0
66	109255.0
67	94849.0
68	83926.5
69	73004.0
70	65072.0
71	57140.0
72	49649.0
73	42158.0
74	33057.5
75	17948.5
76	11940.0
77	9395.0
78	6850.0
79	5207.0
80	3564.0
81	2512.0
82	1460.0
83	1152.5
84	845.0
85	554.5
86	264.0
87	175.0
88	86.0
89	65.0
90	44.0
91	24.0
92	4.0
93	10.0
94	16.0
95	9.0
96	2.0
97	1.5
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4708582.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.343915771200155
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.85591582676531	17.04437941238528
2	5.943501911101383	2.537152083530301
3	2.3382261423816826	1.4972070551103869
4	1.3213375524643987	1.1281006970049554
5	0.9656273094477159	1.0305133979611338
6	0.755315550749514	0.9672834897525171
7	0.5969925433142061	0.8919510992373083
8	0.49283459426197235	0.8415216055248913
9	0.42725756574957713	0.8207414546370287
>10	5.129334918989636	26.425984068196804
>50	1.504619157362926	22.342113442353867
>100	0.6608439368185759	20.930503723656024
>500	0.005120619120722955	0.7589379749149319
>1k	0.002867546707462217	1.1201767125870672
>5k	1.0241238240936491E-4	0.19666373732868606
>10k+	1.0241238240936491E-4	1.4667700458185495
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	67102	1.4250999557828663	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8997	0.19107663411192585	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.123781639567921E-5	0.0	0.0	0.016714161503399536	0.0
2	2.123781639567921E-5	0.0	0.0	0.05303082754001098	0.0
3	4.247563279135842E-5	0.0	0.0	0.09491180147229038	0.0
4	4.247563279135842E-5	0.0	0.0	0.13643173252584323	0.0
5	6.371344918703763E-5	0.0	0.0	0.24347032716006645	0.0
6	6.371344918703763E-5	0.0	0.0	0.4579297971236351	0.0
7	6.371344918703763E-5	0.0	0.0	0.6136879425695464	0.0
8	6.371344918703763E-5	0.0	0.0	0.93149062711449	0.0
9	6.371344918703763E-5	0.0	0.0	1.1233318226166604	0.0
10	6.371344918703763E-5	0.0	0.0	1.3815199565389324	0.0
11	6.371344918703763E-5	0.0	0.0	1.5498721271074816	0.0
12	8.495126558271684E-5	0.0	0.0	1.6714373881563493	0.0
13	1.0618908197839605E-4	0.0	0.0	1.7352570264253655	0.0
14	1.0618908197839605E-4	0.0	0.0	1.7792830198136085	0.0
15	1.2742689837407525E-4	0.0	0.0	1.8110547931415446	0.0
16	1.2742689837407525E-4	0.0	0.0	1.860411478445103	0.0
17	1.4866471476975446E-4	0.0	0.0	1.9146316237032721	0.0
18	1.4866471476975446E-4	0.0	0.0	2.007462119168786	0.0
19	1.4866471476975446E-4	0.0	0.0	2.054057888340906	0.0
20	1.4866471476975446E-4	0.0	0.0	2.1065365326546295	0.0
21	1.4866471476975446E-4	0.0	0.0	2.1637936856573803	0.0
22	1.4866471476975446E-4	0.0	0.0	2.232328119166237	0.0
23	1.6990253116543367E-4	0.0	0.0	2.307701129554503	0.0
24	1.9114034756111288E-4	0.0	0.0	2.3768302219224386	0.0
25	1.9114034756111288E-4	0.0	0.0	2.4344484178039165	0.0
26	1.9114034756111288E-4	0.0	0.0	2.4901764480261788	0.0
27	1.9114034756111288E-4	0.0	0.0	2.554293415724734	0.0
28	1.9114034756111288E-4	0.0	0.0	2.618580285954455	0.0
29	1.9114034756111288E-4	0.0	0.0	2.6975000116807992	0.0
30	1.9114034756111288E-4	0.0	0.0	2.815136276696466	0.0
31	2.123781639567921E-4	0.0	0.0	2.9005122986070964	0.0
32	2.548537967481505E-4	0.0	0.0	2.987799723993338	0.0
33	2.548537967481505E-4	0.0	0.0	3.0760853267501767	0.0
34	2.548537967481505E-4	0.0	0.0	3.170338755914201	0.0
35	2.760916131438297E-4	0.0	0.0	3.2879962587462637	0.0
36	2.9732942953950893E-4	0.0	0.0	3.386815818435359	0.0
37	3.3980506233086735E-4	0.0	0.0	3.488714861501828	0.0
38	3.3980506233086735E-4	0.0	0.0	3.6156108144660113	0.0
39	3.3980506233086735E-4	0.0	0.0	3.8451066584377207	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	43675	0.0	42.568405	1
TACGGGA	830	0.0	39.307228	4
ACGTTAG	220	0.0	38.863632	1
GGGCGAT	7690	0.0	37.773083	7
CGTAAGG	540	0.0	37.5	2
TAGGGAC	3040	0.0	37.154606	5
GTTTTTT	52445	0.0	36.9816	2
ACGGGCT	1655	0.0	36.97885	5
AGGGCGA	4060	0.0	36.687195	6
CGGTCTA	455	0.0	36.593407	31
TCGTAAG	160	0.0	36.5625	1
ACGGGAT	1035	0.0	36.52174	5
ATAGGGC	2335	0.0	36.52034	4
TACGGGC	780	0.0	36.346157	4
CATATGC	3090	0.0	36.33495	33
TCAGACG	3030	0.0	36.311882	22
GGTACCT	3255	0.0	36.082947	8
ACCCGCT	3025	0.0	36.07438	34
GCGTAAG	275	0.0	36.0	1
GTAGCAT	3110	0.0	35.95659	29
>>END_MODULE