FastQCFastQC Report
Thu 26 May 2016
SRR1545256_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545256_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2018483
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101650.5035960174051504No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA58970.29215009489800015No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT39410.1952456374415836No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC35490.17582511222536926No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG31050.15382839488863667No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAACG600.045.0000041
TCTAGCG302.1663218E-645.0000041
CCGTAAG700.045.0000041
TAATGCG1050.045.0000041
GTCGCAA253.891858E-545.035
CATTCGA253.891858E-545.016
TCCTATA253.891858E-545.044
CCGTACA207.0345565E-445.044
CGTAGTG207.0345565E-445.036
GTATGCG502.1827873E-1145.01
CTACGAT207.0345565E-445.031
CACCGTT253.891858E-545.024
TCGACAC207.0345565E-445.034
ACGCGTA253.891858E-545.023
TACGGGA3050.042.786884
GCTACGA5150.042.3786410
ACGTTAG1650.042.2727281
CGACGGT2150.041.86046228
CCCGTAT700.041.78571731
CTTTACG700.041.78571719