##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545256_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2018483
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.38283205754024	31.0	31.0	34.0	30.0	34.0
2	31.639027428023915	31.0	31.0	34.0	30.0	34.0
3	31.71271940363134	31.0	31.0	34.0	30.0	34.0
4	35.467501584110444	37.0	35.0	37.0	33.0	37.0
5	35.34450723637504	37.0	35.0	37.0	33.0	37.0
6	35.33608506982719	37.0	35.0	37.0	33.0	37.0
7	35.93390035982468	37.0	35.0	37.0	35.0	37.0
8	36.029895718715494	37.0	35.0	37.0	35.0	37.0
9	37.693198803259676	39.0	38.0	39.0	35.0	39.0
10	36.96146561551423	39.0	37.0	39.0	32.0	39.0
11	36.612766617306164	39.0	35.0	39.0	32.0	39.0
12	35.609431439353216	37.0	35.0	39.0	31.0	39.0
13	35.155029296754044	37.0	35.0	39.0	30.0	39.0
14	35.94786381653945	38.0	35.0	40.0	30.0	41.0
15	36.404133203004434	38.0	35.0	40.0	31.0	41.0
16	36.3443927939943	38.0	35.0	40.0	31.0	41.0
17	36.43999528358673	38.0	35.0	40.0	31.0	41.0
18	36.5257720773472	37.0	35.0	40.0	31.0	41.0
19	36.44913234344803	37.0	35.0	40.0	31.0	41.0
20	36.31659419474923	37.0	35.0	40.0	31.0	41.0
21	36.17796632421477	37.0	35.0	40.0	31.0	41.0
22	36.068739246255724	36.0	35.0	40.0	31.0	41.0
23	36.02078243908916	36.0	34.0	40.0	31.0	41.0
24	35.84301576976373	36.0	34.0	40.0	31.0	41.0
25	35.83225174549402	36.0	34.0	40.0	31.0	41.0
26	35.70288132226033	36.0	34.0	40.0	30.0	41.0
27	35.66115543207448	36.0	34.0	40.0	30.0	41.0
28	35.720912685417716	36.0	34.0	40.0	30.0	41.0
29	35.74875190923084	36.0	34.0	40.0	30.0	41.0
30	35.66438310354856	36.0	34.0	40.0	30.0	41.0
31	35.53518855496925	36.0	34.0	40.0	30.0	41.0
32	35.39132655563609	36.0	34.0	40.0	30.0	41.0
33	35.21423118252668	36.0	34.0	40.0	29.0	41.0
34	35.055031922488325	35.0	34.0	40.0	29.0	41.0
35	34.89905587512999	35.0	34.0	40.0	27.0	41.0
36	34.66511929998915	35.0	33.0	40.0	26.0	41.0
37	34.48931945426342	35.0	33.0	40.0	26.0	41.0
38	34.572919861103614	35.0	33.0	40.0	27.0	41.0
39	34.5798433774275	35.0	33.0	40.0	27.0	41.0
40	34.41732677461242	35.0	33.0	40.0	25.0	41.0
41	34.46360806605753	35.0	34.0	40.0	26.0	41.0
42	34.402639506996096	35.0	33.0	40.0	26.0	41.0
43	34.26076662523291	35.0	33.0	40.0	26.0	41.0
44	34.2223902802253	35.0	33.0	40.0	26.0	41.0
45	34.16252452956007	35.0	33.0	39.0	25.0	41.0
46	34.07422752631555	35.0	33.0	39.0	24.0	41.0
47	34.12383012390989	35.0	33.0	39.0	25.0	41.0
48	34.09385067895048	35.0	33.0	39.0	25.0	41.0
49	34.08446343120056	35.0	33.0	39.0	25.0	41.0
50	33.876811447012436	35.0	33.0	39.0	24.0	41.0
51	33.68842541651329	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	6.0
14	19.0
15	46.0
16	117.0
17	359.0
18	860.0
19	1681.0
20	2968.0
21	4872.0
22	7408.0
23	11224.0
24	16256.0
25	22644.0
26	30368.0
27	35997.0
28	41075.0
29	47552.0
30	57936.0
31	73713.0
32	94188.0
33	126680.0
34	222307.0
35	328451.0
36	141324.0
37	169373.0
38	234224.0
39	346512.0
40	320.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.2363378834501	24.496119115196908	28.644184766480567	14.623358234872427
2	30.68948314154739	26.272304497981903	29.900821557575664	13.137390802895046
3	29.03096037965145	25.879881079008342	31.364049139873856	13.725109401466348
4	26.175548666993976	28.37591399085353	30.488391529678477	14.960145812474021
5	22.672571431119312	33.55316839428422	30.005305964925142	13.768954209671321
6	20.89014373665768	41.88174980913884	27.003893518052912	10.224212936150565
7	86.95337042719706	5.315080681878421	6.0964595688940655	1.6350893220304554
8	88.81357930683588	3.8194029872929325	5.894228487433384	1.4727892184378069
9	84.68131760336847	5.305915382988116	7.566523968742862	2.4462430449005517
10	54.01462385365643	22.50170053451032	13.637865664461875	9.845809947371368
11	48.01482103143797	20.816127755348944	19.18936151555401	11.979689697659083
12	43.03514074678855	22.023717811841863	23.26648279921109	11.674658642158493
13	20.702973470670795	43.776043692218366	21.770111514439307	13.750871322671532
14	14.150874691538151	45.14979814048471	26.02816075240663	14.671166415570505
15	13.143880825352506	23.917119936110435	49.11777805411292	13.821221184424145
16	15.157868557723795	18.752102445252202	46.59588413675022	19.494144860273778
17	14.860417452116268	19.444305451172987	29.711075099468264	35.98420199724248
18	20.010869549062342	23.87416688671641	37.30613535016148	18.808828214059766
19	31.81760757955356	23.836168052938767	24.770780828969084	19.575443538538593
20	33.14380155790264	23.300716429120286	25.183962411375276	18.371519601601797
21	21.116650474638625	31.28195778711042	27.56282812389304	20.038563614357912
22	21.4375845622678	25.86769370859205	22.946093675299718	29.74862805384043
23	17.690859918067183	32.518728173583824	24.935359871745266	24.855052036603727
24	17.122710471180586	23.896758109927106	43.161076907756964	15.819454511135342
25	16.497488460393274	26.190708566780103	38.71570877733427	18.59609419549236
26	15.224304589139468	37.51079399727419	27.0325288843156	20.232372529270744
27	16.237937104251063	39.375560755280084	27.196562963373978	17.189939177094878
28	14.514315949155876	30.9809396462591	38.29940603908975	16.205338365495276
29	15.146523403962284	25.489637514905993	37.59357894022392	21.7702601409078
30	17.600841820317537	35.16333801176428	30.488242903209983	16.747577264708198
31	30.839893127660723	28.44339040754864	24.29240176905131	16.424314695739326
32	33.031192236942296	24.751707098846015	26.569953772214085	15.647146891997604
33	30.84410421093465	27.295151854139966	23.127368424703107	18.73337551022228
34	19.6072000606396	27.358070392468008	27.01741852668563	26.01731102020676
35	20.952071431862443	24.873333092228172	31.990856499658406	22.18373897625098
36	32.02781494815661	26.9989888445927	23.969337368707095	17.0038588385436
37	19.67423059792924	38.24277935459451	26.719571083828797	15.363418963647451
38	19.354980943609633	37.380696295188024	23.742037956227524	19.522284804974824
39	20.745084303410035	35.89646283867637	25.833757331619832	17.52469552629376
40	25.864077131192087	26.1751027875885	25.668137903564208	22.292682177655198
41	16.914137993730936	24.966274177191487	27.51764567747165	30.601942151605932
42	19.30296167963763	26.068091730274666	24.520791108966485	30.10815548112122
43	19.43850901890182	25.55102024639296	27.20984026122588	27.800630473479345
44	17.100763296000014	30.373354643066104	31.27665677640089	21.249225284532987
45	16.429318453511872	41.265643555085674	22.82957052400243	19.475467467400023
46	19.887311411589792	37.40635913208087	24.295423840577303	18.41090561575203
47	19.915500898447004	29.15298271028292	26.22811289468378	24.703403496586297
48	19.998186757084405	25.7238728292485	34.019459168098024	20.258481245569072
49	20.769855381491944	27.04253639986069	33.83407241973303	18.353535798914333
50	18.77246427143553	37.35651972298008	25.881862765254898	17.989153240329493
51	17.161105642207538	35.882987372199814	25.086512990201054	21.86939399539159
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6727.0
1	6005.5
2	5284.0
3	11132.5
4	16981.0
5	12259.0
6	7537.0
7	7663.0
8	7789.0
9	8462.0
10	9135.0
11	9559.0
12	9983.0
13	10225.0
14	10467.0
15	9917.0
16	9367.0
17	9229.5
18	9092.0
19	8446.5
20	7801.0
21	7661.0
22	7521.0
23	8000.5
24	8480.0
25	11499.0
26	15849.0
27	17180.0
28	22889.0
29	28598.0
30	33449.0
31	38300.0
32	44730.0
33	51160.0
34	59266.0
35	67372.0
36	69295.5
37	71219.0
38	85415.5
39	99612.0
40	133651.5
41	167691.0
42	199649.0
43	231607.0
44	228297.0
45	224987.0
46	203928.0
47	182869.0
48	162492.0
49	142115.0
50	126026.0
51	109937.0
52	100106.5
53	90276.0
54	77248.0
55	64220.0
56	62266.5
57	60313.0
58	57120.5
59	53928.0
60	52201.0
61	50474.0
62	45608.5
63	40743.0
64	34197.5
65	27652.0
66	24467.0
67	21282.0
68	18040.5
69	14799.0
70	12961.0
71	11123.0
72	10012.0
73	8901.0
74	7069.0
75	4208.0
76	3179.0
77	2228.5
78	1278.0
79	1122.5
80	967.0
81	714.5
82	462.0
83	328.0
84	194.0
85	146.0
86	98.0
87	60.5
88	23.0
89	13.0
90	3.0
91	1.5
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2018483.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.300005201419395
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.33827852682958	12.769143474575637
2	7.846245315803412	2.5578767891841636
3	2.780031077121058	1.3594356305154245
4	1.4203303292210563	0.9260556701614776
5	0.8946208952258804	0.7291162622740164
6	0.6271045333797808	0.6133084293554466
7	0.456594487853439	0.5209744768865335
8	0.34235496113676694	0.44643101178088296
9	0.3105169632156117	0.4555285303989083
>10	4.093241965709861	16.09089953399041
>50	1.537884097379407	17.66763031881818
>100	1.3235726643640058	41.412401584708185
>500	0.025139081944216154	2.553003109668624
>1k	0.003456623767329721	1.0754851492428645
>5k	3.142385243027019E-4	0.3020496225691848
>10k+	3.142385243027019E-4	0.5206604058700633
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10165	0.5035960174051504	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5897	0.29215009489800015	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	3941	0.1952456374415836	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3549	0.17582511222536926	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3105	0.15382839488863667	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.025415126112035624	0.0
2	0.0	0.0	0.0	0.08922542325102564	0.0
3	0.0	0.0	0.0	0.1217250776944864	0.0
4	4.954215616381213E-5	0.0	0.0	0.16155697125019136	0.0
5	4.954215616381213E-5	0.0	0.0	0.2778819539228222	0.0
6	4.954215616381213E-5	0.0	0.0	0.402678645299465	0.0
7	4.954215616381213E-5	0.0	0.0	0.4986913439449329	0.0
8	4.954215616381213E-5	0.0	0.0	0.7726594675308139	0.0
9	4.954215616381213E-5	0.0	0.0	0.8941368344444813	0.0
10	4.954215616381213E-5	0.0	0.0	1.096714710998309	0.0
11	4.954215616381213E-5	0.0	0.0	1.2811601583961816	0.0
12	4.954215616381213E-5	0.0	0.0	1.4356821434711118	0.0
13	4.954215616381213E-5	0.0	0.0	1.4961235739909626	0.0
14	4.954215616381213E-5	0.0	0.0	1.5180212070153676	0.0
15	4.954215616381213E-5	0.0	0.0	1.550322692834173	0.0
16	9.908431232762426E-5	0.0	0.0	1.62760845644972	0.0
17	9.908431232762426E-5	0.0	0.0	1.7276340697444565	0.0
18	9.908431232762426E-5	0.0	0.0	1.8783413087947731	0.0
19	9.908431232762426E-5	0.0	0.0	1.937742354035184	0.0
20	9.908431232762426E-5	0.0	0.0	2.007943589319306	0.0
21	9.908431232762426E-5	0.0	0.0	2.1050462154003773	0.0
22	9.908431232762426E-5	0.0	0.0	2.2063103825992094	0.0
23	1.4862646849143638E-4	0.0	0.0	2.3328905915977494	0.0
24	1.4862646849143638E-4	0.0	0.0	2.425534423624078	0.0
25	1.4862646849143638E-4	0.0	0.0	2.5035633195820823	0.0
26	1.4862646849143638E-4	0.0	0.0	2.5791646498880594	0.0
27	1.4862646849143638E-4	0.0	0.0	2.6543201007885626	0.0
28	1.4862646849143638E-4	0.0	0.0	2.7379472603930775	0.0
29	1.4862646849143638E-4	0.0	0.0	2.8296993336084575	0.0
30	1.4862646849143638E-4	0.0	0.0	2.9638594925000605	0.0
31	1.4862646849143638E-4	0.0	0.0	3.076964235022044	0.0
32	1.4862646849143638E-4	0.0	0.0	3.176395342442815	0.0
33	1.4862646849143638E-4	0.0	0.0	3.2692373430937987	0.0
34	1.4862646849143638E-4	0.0	0.0	3.37282999163233	0.0
35	1.4862646849143638E-4	0.0	0.0	3.5223977610908785	0.0
36	1.4862646849143638E-4	0.0	0.0	3.637583274171742	0.0
37	1.4862646849143638E-4	0.0	0.0	3.751976112753984	0.0
38	1.4862646849143638E-4	0.0	0.0	3.86433772293351	0.0
39	2.9725293698287277E-4	0.0	0.0	3.9818021751979087	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAACG	60	0.0	45.000004	1
TCTAGCG	30	2.1663218E-6	45.000004	1
CCGTAAG	70	0.0	45.000004	1
TAATGCG	105	0.0	45.000004	1
GTCGCAA	25	3.891858E-5	45.0	35
CATTCGA	25	3.891858E-5	45.0	16
TCCTATA	25	3.891858E-5	45.0	44
CCGTACA	20	7.0345565E-4	45.0	44
CGTAGTG	20	7.0345565E-4	45.0	36
GTATGCG	50	2.1827873E-11	45.0	1
CTACGAT	20	7.0345565E-4	45.0	31
CACCGTT	25	3.891858E-5	45.0	24
TCGACAC	20	7.0345565E-4	45.0	34
ACGCGTA	25	3.891858E-5	45.0	23
TACGGGA	305	0.0	42.78688	4
GCTACGA	515	0.0	42.37864	10
ACGTTAG	165	0.0	42.272728	1
CGACGGT	215	0.0	41.860462	28
CCCGTAT	70	0.0	41.785717	31
CTTTACG	70	0.0	41.785717	19
>>END_MODULE