FastQCFastQC Report
Thu 26 May 2016
SRR1545255_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545255_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3214657
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159420.49591604951943546No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA86660.26957774966349446No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC50180.15609752455705228No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT33510.10424129230583543No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAA351.2128476E-745.018
ACGTTAC253.892376E-545.031
CGCGAAA207.0351816E-445.030
CGTTAGG6150.041.7073172
CGGTCTA2450.040.40816531
ACGTTAG1450.040.3448261
CGTTTTT114850.040.102311
GGGCGAT54500.040.004597
TAGTAGG13350.038.9325832
ACGGGAT7150.038.7062955
CGTAAGT1050.038.5714337
TAATGCG1050.038.571431
TTACGTA356.2517956E-638.57142624
ACGGTAC356.2517956E-638.57142615
ATAGGGC11500.038.5434764
TGGGCGA18500.038.432436
CATATGC16750.038.41790833
AGGGCGA25600.038.232426
TCGTTAG1650.038.1818161
ACATACG10550.038.17535417