##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545255_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3214657
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14963991492716	31.0	31.0	33.0	30.0	34.0
2	31.40947385677539	31.0	31.0	34.0	30.0	34.0
3	31.508562810900198	31.0	31.0	34.0	30.0	34.0
4	35.285720062824744	37.0	35.0	37.0	33.0	37.0
5	35.13488001985904	37.0	35.0	37.0	32.0	37.0
6	35.12155542566439	37.0	35.0	37.0	32.0	37.0
7	35.78804799392284	37.0	35.0	37.0	35.0	37.0
8	35.86665233647011	37.0	35.0	37.0	35.0	37.0
9	37.49214737373225	39.0	37.0	39.0	35.0	39.0
10	36.711105103903776	39.0	37.0	39.0	32.0	39.0
11	36.39844561954821	38.0	35.0	39.0	32.0	39.0
12	35.55121152894383	37.0	35.0	39.0	31.0	39.0
13	35.14553869977419	37.0	35.0	39.0	30.0	39.0
14	35.87050002535263	38.0	34.0	40.0	27.0	41.0
15	36.301882284797415	38.0	35.0	40.0	30.0	41.0
16	36.225152792350784	38.0	35.0	40.0	31.0	41.0
17	36.27863936961237	38.0	35.0	40.0	31.0	41.0
18	36.332061865387196	38.0	35.0	40.0	31.0	41.0
19	36.24940110251265	37.0	35.0	40.0	31.0	41.0
20	36.11080031244391	37.0	34.0	40.0	30.0	41.0
21	35.970989128855734	37.0	34.0	40.0	30.0	41.0
22	35.89340666826974	37.0	34.0	40.0	30.0	41.0
23	35.82317864705317	37.0	34.0	40.0	30.0	41.0
24	35.627480319051145	36.0	34.0	40.0	30.0	41.0
25	35.59345273850367	36.0	34.0	40.0	30.0	41.0
26	35.457039429089946	36.0	34.0	40.0	29.0	41.0
27	35.411497089736166	36.0	34.0	40.0	29.0	41.0
28	35.441428432333524	36.0	34.0	40.0	29.0	41.0
29	35.40418993379387	36.0	34.0	40.0	29.0	41.0
30	35.29828874433571	36.0	34.0	40.0	29.0	41.0
31	35.14927937879531	36.0	34.0	40.0	29.0	41.0
32	35.010030619129815	36.0	34.0	40.0	27.0	41.0
33	34.81681840395414	36.0	33.0	40.0	27.0	41.0
34	34.63639417829025	36.0	34.0	40.0	26.0	41.0
35	34.46815010123942	36.0	33.0	40.0	25.0	41.0
36	34.26288154537171	35.0	33.0	40.0	24.0	41.0
37	34.08602721845597	35.0	33.0	40.0	23.0	41.0
38	34.13244274583572	35.0	33.0	40.0	24.0	41.0
39	34.12055718541667	35.0	33.0	40.0	24.0	41.0
40	33.97267297879681	35.0	33.0	40.0	23.0	41.0
41	33.94092433500681	35.0	33.0	40.0	23.0	41.0
42	33.8628811720815	35.0	33.0	40.0	23.0	41.0
43	33.69431544329613	35.0	33.0	40.0	23.0	41.0
44	33.65969650883438	35.0	33.0	39.0	23.0	41.0
45	33.60502131331585	35.0	33.0	39.0	23.0	41.0
46	33.52331710661511	35.0	32.0	39.0	23.0	41.0
47	33.534049511347554	35.0	32.0	39.0	23.0	41.0
48	33.51359569621269	35.0	32.0	39.0	23.0	41.0
49	33.50131786999359	35.0	32.0	39.0	23.0	41.0
50	33.30379944112234	35.0	32.0	39.0	23.0	41.0
51	33.099322882659024	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	2.0
12	7.0
13	8.0
14	42.0
15	141.0
16	419.0
17	1040.0
18	2232.0
19	4206.0
20	7451.0
21	11826.0
22	17286.0
23	24523.0
24	33519.0
25	45099.0
26	57793.0
27	67669.0
28	76834.0
29	88229.0
30	105211.0
31	128317.0
32	157484.0
33	203352.0
34	324103.0
35	446459.0
36	240027.0
37	287952.0
38	380944.0
39	502176.0
40	303.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.36392840666983	23.493797316478865	26.923432266646174	14.218842010205131
2	32.507107290140134	25.024722699809033	29.01597277718898	13.452197232861856
3	29.442643491980636	26.14549546032438	30.652010463324704	13.759850584370275
4	26.403407890795194	27.939372692016594	30.223846587676384	15.433372829511827
5	22.88088589233626	32.61570363494457	29.86091517695356	14.642495295765615
6	21.29707772866592	41.14796695261734	27.512359794528624	10.042595524188117
7	85.38839446945661	5.428821799650787	7.210224916686291	1.9725588142063057
8	86.44645447399209	4.286056024017492	7.249109313995242	2.0183801879951733
9	82.49716843818796	5.720081489253753	8.682885919088724	3.0998641534695617
10	50.236681549540116	22.764077162820172	14.909086723715781	12.090154563923928
11	43.60126134763367	22.144290977233343	20.629914793397866	13.624532881735126
12	38.52432778986996	22.151881211588048	25.162653433943344	14.16113756459865
13	22.28626568868778	38.10944682434238	24.68303772377582	14.92124976319402
14	16.247301033982787	40.11557065030577	28.635621156471746	15.001507159239694
15	15.452130662773664	23.440416815853137	45.93280091779621	15.174651603576992
16	18.231431844828236	18.972195167322674	43.52551454167583	19.27085844617326
17	18.01806537991456	19.572912444469193	29.71791391741016	32.691108258206086
18	22.155987403943872	22.210176700033628	35.79731834531647	19.83651755070603
19	31.451784747175203	23.478056912448203	25.98118555105568	19.088972789320913
20	32.29414522295847	23.402870041811617	25.595265684643802	18.707719050586114
21	23.023887151879656	29.463236668795457	28.627657631902874	18.88521854742201
22	22.287230021741046	24.26361506064255	25.396893043332465	28.052261874283946
23	19.23822665995159	31.030713385596037	26.029433311236627	23.701626643215747
24	18.54966175240469	24.448082641476212	39.87719374104298	17.12506186507612
25	18.85019148232611	25.533641691788578	36.07579284508425	19.540373980801064
26	17.39435964707899	33.742480146404425	27.83114963742633	21.032010569090264
27	17.69025435684118	34.620520945158376	28.722411131265325	18.966813566735112
28	16.842107882738343	28.385081207730718	36.80663286938544	17.966178040145497
29	17.31711968026449	25.5706596380267	36.23338353049797	20.878837151210845
30	19.77529795558282	31.54672489164474	30.509849106763177	18.16812804600926
31	29.862781628024386	26.603895843320142	25.624631181491527	17.908691347163945
32	31.66636440528492	24.442545503299417	25.887894105032043	18.003195986383616
33	30.234049853530255	24.965742845970816	24.573757013578742	20.22645028692019
34	20.66743045992154	25.46473853975712	27.501721023424896	26.366109976896446
35	20.918965849233683	25.06413592492138	32.003196608533976	22.01370161731096
36	30.879904139073	25.975337337700417	24.519847685149614	18.62491083807697
37	20.507413388115747	34.215687707895434	26.631146028954255	18.645752875034567
38	21.060909453170275	34.68796204385102	24.21938017026389	20.031748332714812
39	20.795904508630315	33.08617373486503	25.01128425209906	21.1066375044056
40	26.0105510479034	24.284488205117995	26.99373525698076	22.711225489997844
41	18.713318403798603	23.91894376289601	27.434590999910725	29.93314683339467
42	20.898901500222262	24.665648621299255	25.423707723716717	29.011742154761766
43	20.188281362521725	24.43025803374979	27.913149054471443	27.468311549257045
44	18.7544114348747	28.430809258966043	31.330278782464195	21.484500523695065
45	17.904865122468742	36.95115839730335	24.331616094656443	20.812360385571463
46	21.050955047459187	33.35152086210131	25.4194771012895	20.17804698915001
47	20.076978663664583	28.168697313585866	26.701417911770992	25.052906110978558
48	20.83985943134835	25.622578085313613	32.66423136278614	20.873331120551896
49	21.152956598480024	26.1206405535645	32.846085912120635	19.880316935834834
50	19.979363272660194	34.315853915363284	26.242177625793357	19.46260518618316
51	18.517932084200588	34.581169935081725	25.67147910336935	21.229418877348348
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	9918.0
1	9064.5
2	8211.0
3	18347.0
4	28483.0
5	20941.5
6	13400.0
7	13621.0
8	13842.0
9	14577.0
10	15312.0
11	15639.5
12	15967.0
13	16076.5
14	16186.0
15	15376.5
16	14567.0
17	14402.5
18	14238.0
19	13358.0
20	12478.0
21	13807.5
22	15137.0
23	15954.0
24	16771.0
25	18852.5
26	23846.5
27	26759.0
28	32456.0
29	38153.0
30	44872.5
31	51592.0
32	61183.5
33	70775.0
34	86431.0
35	102087.0
36	102084.0
37	102081.0
38	124871.5
39	147662.0
40	183591.5
41	219521.0
42	272830.5
43	326140.0
44	316794.5
45	307449.0
46	286629.5
47	265810.0
48	247541.5
49	229273.0
50	211502.5
51	193732.0
52	174176.0
53	154620.0
54	144603.5
55	134587.0
56	127700.5
57	120814.0
58	113997.0
59	107180.0
60	101006.5
61	94833.0
62	86487.0
63	78141.0
64	72151.0
65	66161.0
66	58566.0
67	50971.0
68	42663.5
69	34356.0
70	31235.5
71	28115.0
72	23226.0
73	18337.0
74	16126.5
75	10793.5
76	7671.0
77	6044.5
78	4418.0
79	3425.0
80	2432.0
81	1616.0
82	800.0
83	591.5
84	383.0
85	293.0
86	203.0
87	167.5
88	132.0
89	82.5
90	33.0
91	26.5
92	20.0
93	17.5
94	15.0
95	28.0
96	41.0
97	20.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3214657.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.907845471776398
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.11481185069769	14.167758251509582
2	7.184532951378343	2.57319011760338
3	2.590890441697074	1.3919179699264108
4	1.5012144736391875	1.0753406725569883
5	1.0276177557823605	0.9201209987302084
6	0.7074493914706675	0.7601336628935378
7	0.5685427867321452	0.7126963458224665
8	0.4358944021238884	0.6244743676197564
9	0.3621284096910231	0.5836445641518277
>10	3.9130565659451313	16.745872944512186
>50	1.4671086075149469	18.593879986269112
>100	1.1033795445541996	37.86038493799887
>500	0.01995679141427815	2.2889906959252655
>1k	0.0028766546182743286	0.7477356728017606
>5k	3.595818272842911E-4	0.44057935526262876
>10k+	1.7979091364214554E-4	0.5132794564160208
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15942	0.49591604951943546	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8666	0.26957774966349446	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5018	0.15609752455705228	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	3351	0.10424129230583543	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.110751784716068E-5	0.0	0.0	0.0230195632068989	0.0
2	3.110751784716068E-5	0.0	0.0	0.06846764678160065	0.0
3	3.110751784716068E-5	0.0	0.0	0.10445904493076555	0.0
4	3.110751784716068E-5	0.0	0.0	0.1517424720584498	0.0
5	3.110751784716068E-5	0.0	0.0	0.28245626205221896	0.0
6	3.110751784716068E-5	0.0	0.0	0.4439976022325243	0.0
7	3.110751784716068E-5	0.0	0.0	0.5644148038188833	0.0
8	3.110751784716068E-5	0.0	0.0	0.8755210898083372	0.0
9	3.110751784716068E-5	0.0	0.0	1.0280723573308133	0.0
10	6.221503569432136E-5	0.0	0.0	1.2497445295096803	0.0
11	9.332255354148203E-5	0.0	0.0	1.4286438646487012	0.0
12	1.2443007138864272E-4	0.0	0.0	1.5841192388488103	0.0
13	1.5553758923580338E-4	0.0	0.0	1.6518403052020791	0.0
14	1.5553758923580338E-4	0.0	0.0	1.6813613396390346	0.0
15	1.8664510708296406E-4	0.0	0.0	1.7163261896992432	0.0
16	1.8664510708296406E-4	0.0	0.0	1.7934728339602017	0.0
17	1.8664510708296406E-4	0.0	0.0	1.883591313163426	0.0
18	1.8664510708296406E-4	0.0	0.0	2.025472702064326	0.0
19	1.8664510708296406E-4	0.0	0.0	2.0867234047053853	0.0
20	2.1775262493012475E-4	0.0	0.0	2.1578351905039947	0.0
21	2.1775262493012475E-4	0.0	0.0	2.2510022064562407	0.0
22	2.1775262493012475E-4	0.0	0.0	2.3455379531937623	0.0
23	2.4886014277728544E-4	0.0	0.0	2.4606046617104096	0.0
24	3.7329021416592813E-4	0.0	0.0	2.553989430287586	0.0
25	3.7329021416592813E-4	0.0	0.0	2.630731676816531	0.0
26	3.7329021416592813E-4	0.0	0.0	2.7108957503086644	0.0
27	3.7329021416592813E-4	0.0	0.0	2.7935795327464175	0.0
28	3.7329021416592813E-4	0.0	0.0	2.880587260164926	0.0
29	3.7329021416592813E-4	0.0	0.0	2.9880015192911715	0.0
30	4.043977320130888E-4	0.0	0.0	3.1357622290651848	0.0
31	4.043977320130888E-4	0.0	0.0	3.2531620014203693	0.0
32	4.043977320130888E-4	0.0	0.0	3.367482129508685	0.0
33	4.355052498602495E-4	0.0	0.0	3.4814600749006814	0.0
34	4.355052498602495E-4	0.0	0.0	3.6052368884145336	0.0
35	4.977202855545709E-4	0.0	0.0	3.761334412971586	0.0
36	4.977202855545709E-4	0.0	0.0	3.8818760446293337	0.0
37	4.977202855545709E-4	0.0	0.0	4.00758152424971	0.0
38	5.288278034017315E-4	0.0	0.0	4.1640834465387755	0.0
39	5.599353212488922E-4	0.0	0.0	4.445419837948497	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGAAA	35	1.2128476E-7	45.0	18
ACGTTAC	25	3.892376E-5	45.0	31
CGCGAAA	20	7.0351816E-4	45.0	30
CGTTAGG	615	0.0	41.707317	2
CGGTCTA	245	0.0	40.408165	31
ACGTTAG	145	0.0	40.344826	1
CGTTTTT	11485	0.0	40.10231	1
GGGCGAT	5450	0.0	40.00459	7
TAGTAGG	1335	0.0	38.932583	2
ACGGGAT	715	0.0	38.706295	5
CGTAAGT	105	0.0	38.57143	37
TAATGCG	105	0.0	38.57143	1
TTACGTA	35	6.2517956E-6	38.571426	24
ACGGTAC	35	6.2517956E-6	38.571426	15
ATAGGGC	1150	0.0	38.543476	4
TGGGCGA	1850	0.0	38.43243	6
CATATGC	1675	0.0	38.417908	33
AGGGCGA	2560	0.0	38.23242	6
TCGTTAG	165	0.0	38.181816	1
ACATACG	1055	0.0	38.175354	17
>>END_MODULE