##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545253_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2637256
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.266779940968945	31.0	31.0	34.0	30.0	34.0
2	31.518365300903668	31.0	31.0	34.0	30.0	34.0
3	31.60527646917857	31.0	31.0	34.0	30.0	34.0
4	35.34520691203281	37.0	35.0	37.0	33.0	37.0
5	35.190826753261724	37.0	35.0	37.0	32.0	37.0
6	35.17884725639073	37.0	35.0	37.0	32.0	37.0
7	35.84012170225416	37.0	35.0	37.0	35.0	37.0
8	35.969153544441646	37.0	35.0	37.0	35.0	37.0
9	37.669258502018764	39.0	37.0	39.0	35.0	39.0
10	36.9060534889294	39.0	37.0	39.0	32.0	39.0
11	36.49397441886567	39.0	35.0	39.0	32.0	39.0
12	35.02229438476962	35.0	34.0	39.0	30.0	39.0
13	34.278093973432995	35.0	33.0	39.0	25.0	39.0
14	34.92084310358949	35.0	33.0	40.0	25.0	41.0
15	35.58149531179377	35.0	33.0	40.0	30.0	41.0
16	35.6776130189864	35.0	34.0	40.0	31.0	41.0
17	35.797256694078996	35.0	34.0	40.0	31.0	41.0
18	35.93676457651438	36.0	35.0	40.0	31.0	41.0
19	35.82795071847405	36.0	35.0	40.0	31.0	41.0
20	35.6200990726725	35.0	34.0	40.0	31.0	41.0
21	35.45354148402734	35.0	34.0	40.0	30.0	41.0
22	35.321310483320545	35.0	34.0	40.0	30.0	41.0
23	35.32138935317618	35.0	34.0	40.0	30.0	41.0
24	35.15297415192154	35.0	34.0	40.0	30.0	41.0
25	35.15268180260089	35.0	34.0	39.0	30.0	41.0
26	35.0294207312449	35.0	34.0	39.0	29.0	41.0
27	34.9571209620909	35.0	34.0	39.0	29.0	41.0
28	35.107770728363114	35.0	34.0	39.0	29.0	41.0
29	35.16614428026706	36.0	34.0	39.0	30.0	41.0
30	35.100171162754016	36.0	34.0	39.0	29.0	41.0
31	34.89134539839894	35.0	34.0	39.0	29.0	41.0
32	34.70716039701872	35.0	34.0	39.0	29.0	41.0
33	34.51068041934496	35.0	33.0	39.0	27.0	41.0
34	34.410597605996536	35.0	34.0	39.0	27.0	41.0
35	34.237920778263465	35.0	33.0	39.0	27.0	41.0
36	33.93838368364694	35.0	33.0	39.0	24.0	41.0
37	33.76860494392657	35.0	33.0	39.0	24.0	41.0
38	33.936620866537034	35.0	33.0	39.0	25.0	41.0
39	33.999260215921396	35.0	33.0	39.0	25.0	41.0
40	33.792098681356684	35.0	33.0	39.0	23.0	41.0
41	33.88302652453914	35.0	33.0	39.0	24.0	41.0
42	33.82087556156854	35.0	33.0	39.0	24.0	41.0
43	33.69879526295513	35.0	33.0	39.0	24.0	41.0
44	33.66775883721565	35.0	33.0	38.0	24.0	41.0
45	33.588472639743735	35.0	33.0	38.0	23.0	41.0
46	33.55447480259785	35.0	33.0	38.0	23.0	41.0
47	33.58378215842527	35.0	33.0	38.0	24.0	40.0
48	33.64386961296135	35.0	33.0	38.0	24.0	41.0
49	33.69238329536458	35.0	33.0	38.0	24.0	40.0
50	33.44872549346745	35.0	33.0	38.0	24.0	40.0
51	33.242196813657834	35.0	32.0	38.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	3.0
14	20.0
15	78.0
16	247.0
17	669.0
18	1592.0
19	3008.0
20	5256.0
21	8457.0
22	12729.0
23	17947.0
24	25698.0
25	35649.0
26	45687.0
27	53570.0
28	61110.0
29	70555.0
30	85667.0
31	107377.0
32	137562.0
33	183524.0
34	329686.0
35	517737.0
36	156718.0
37	173321.0
38	234721.0
39	368284.0
40	382.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.121838759680514	25.112237871484606	30.15069450974801	14.615228859086868
2	28.2437503223047	28.253154035861517	30.963054022817655	12.54004161901613
3	26.74491971958733	26.469709425251093	33.24129322295598	13.544077632205594
4	24.13755054496037	29.371703012525142	32.02176808015604	14.46897836235845
5	21.506179149843625	33.765664008348075	30.27358739538369	14.454569446424617
6	19.84763708945965	40.55317344997983	28.423710098678324	11.175479361882198
7	86.42585323533248	5.6847344360957	6.199398162332364	1.690014166239455
8	89.3717560980049	3.9454266100826008	5.261415653239579	1.4214016386729236
9	86.19212545160576	5.027725787712684	6.591396512132307	2.1887522485492497
10	60.90523635172316	20.095394607121946	11.705234531649563	7.2941345095053345
11	56.305758712843954	16.23524602844775	17.36964481263859	10.089350446069703
12	51.62236809775008	20.336402685215237	19.122754863388312	8.918474353646367
13	18.792714852103853	54.5468850957207	17.225100634902336	9.435299417273106
14	10.888969443997851	53.433189648634794	23.439476486165926	12.238364421201432
15	8.230372781406128	22.781481964587435	57.09726321600937	11.89088203799707
16	9.298490552301333	16.037085516157703	54.5005869737333	20.16383695780766
17	9.544010896173901	16.78547702612109	28.64405275786651	45.026459319838494
18	16.55781615436651	21.708358991315215	42.037064281965804	19.696760572352474
19	31.607435910658655	22.97994582247609	24.513547414433788	20.899070852431468
20	33.87335169585357	21.338618624813062	26.03418098205104	18.753848697282326
21	17.66119785109978	33.857501888326354	27.734963917041043	20.746336343532825
22	19.54349520865627	26.19586418610859	19.8918117922568	34.368828812978336
23	13.752703567647584	35.907170179914274	22.967281143734244	27.372845108703896
24	14.00914435307001	21.968174496522142	50.89077435030956	13.131906800098283
25	10.998856387093252	26.749431985366606	43.970134109089145	18.281577518450995
26	11.616619698656482	41.74672462589904	26.505049187488815	20.131606487955665
27	11.024944108573456	45.86103889800611	25.79897438853111	17.315042604889324
28	9.227431845827633	31.925455852598304	43.232245940477526	15.614866361096533
29	9.205098026130191	23.914477775384718	42.8917025878413	23.98872161064379
30	13.839498327049023	39.883348450055664	30.761253363344327	15.515899859550988
31	31.65327901424814	30.40743864076904	22.775301298015815	15.163981046966999
32	34.25204075751463	24.827396354392594	25.2974303594342	15.623132528658576
33	32.57878643559821	28.186190494969015	20.90346936361127	18.331553705821506
34	16.346801372335488	25.281011778909594	26.830235669195556	31.54195117955936
35	17.039036028356747	24.157647190868083	35.30772135886694	23.495595421908224
36	35.328652205170826	24.55726709883303	23.790257752755135	16.323822943241005
37	15.936185186421037	39.947581880560705	28.99236934146704	15.123863591551217
38	15.482456007304563	39.468409589361066	24.559352599823452	20.489781803510922
39	16.53752991745966	39.932793782628615	25.827299283801043	17.702377016110685
40	26.650275892821934	24.447721419536066	24.961968045574643	23.940034642067364
41	13.58802482580379	22.658892424550366	27.644491092256494	36.10859165738935
42	16.88531564626263	22.804498311881744	24.914684050391774	35.39550199146386
43	17.2385236776407	23.372020008675683	28.4484327649648	30.94102354871882
44	14.082326478733956	29.642211450082968	33.190331162389995	23.08513090879308
45	11.614837543264667	47.79892433650733	20.81151772903351	19.774720391194485
46	18.869954225149172	40.69517710832775	23.744111303567042	16.69075736295604
47	16.68165699499783	29.964667821402248	26.28599574709471	27.067679436505216
48	17.86409813836806	24.414087976290507	37.415101150589855	20.306712734751574
49	18.997511049363432	24.390654528798112	38.29658554194208	18.315248879896377
50	17.160260513200086	40.23469848964226	25.754761767534134	16.850279229623517
51	14.252389604953027	38.572516282075	24.034754305232408	23.14033980773956
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	15435.0
1	12033.0
2	8631.0
3	13269.0
4	17907.0
5	12774.0
6	7641.0
7	8312.5
8	8984.0
9	10523.0
10	12062.0
11	12826.0
12	13590.0
13	13532.5
14	13475.0
15	12656.0
16	11837.0
17	11193.5
18	10550.0
19	9402.0
20	8254.0
21	8298.5
22	8343.0
23	9077.0
24	9811.0
25	10774.0
26	14249.0
27	16761.0
28	21795.5
29	26830.0
30	36141.5
31	45453.0
32	55542.0
33	65631.0
34	75296.0
35	84961.0
36	89689.5
37	94418.0
38	112224.0
39	130030.0
40	187887.0
41	245744.0
42	312677.0
43	379610.0
44	369328.0
45	359046.0
46	322923.0
47	286800.0
48	252774.5
49	218749.0
50	192075.5
51	165402.0
52	139662.5
53	113923.0
54	91686.0
55	69449.0
56	58243.0
57	47037.0
58	40213.0
59	33389.0
60	30288.0
61	27187.0
62	24706.0
63	22225.0
64	18628.5
65	15032.0
66	13029.0
67	11026.0
68	9164.0
69	7302.0
70	6198.0
71	5094.0
72	4496.5
73	3899.0
74	3070.5
75	1521.0
76	800.0
77	668.5
78	537.0
79	430.5
80	324.0
81	194.0
82	64.0
83	45.0
84	26.0
85	15.5
86	5.0
87	3.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2637256.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.849449640371532
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.0189986073492	11.585391908120485
2	9.374058398442628	2.7839921622715122
3	3.6331917362066806	1.6185269316184532
4	1.7606194262409125	1.0457691802329698
5	1.067618643182754	0.7926774638532044
6	0.6771084509942012	0.6032812706465019
7	0.47956771028284173	0.49849215940953395
8	0.34420371537254835	0.4088988589962746
9	0.24879647148027734	0.3325041606553659
>10	2.074633748083102	6.829391526546321
>50	0.754307775241412	8.186277377170722
>100	1.4213093526386311	49.448932104034604
>500	0.13213508733128956	11.972303087195012
>1k	0.012395906396348523	2.242745805571004
>5k	2.6374268928401113E-4	0.23580847804667796
>10k+	7.912280678520334E-4	1.4150075256313694
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	14186	0.5379075827299283	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	11101	0.42092993626708974	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10843	0.4111470407120128	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	6021	0.22830548115162125	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4325	0.16399621424692937	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.045994776388791986	0.0
2	0.0	0.0	0.0	0.20217984147159015	0.0
3	0.0	0.0	0.0	0.2934110302526565	0.0
4	0.0	0.0	0.0	0.3900645216088237	0.0
5	0.0	0.0	0.0	0.6950026846085476	0.0
6	0.0	0.0	0.0	0.9451490488598756	0.0
7	0.0	0.0	0.0	1.109410690505586	0.0
8	0.0	0.0	0.0	1.4965175925279912	0.0
9	0.0	0.0	0.0	1.6652914999529815	0.0
10	3.7918199825879624E-5	0.0	0.0	1.9641627509805646	0.0
11	7.583639965175925E-5	0.0	0.0	2.2940131712658913	0.0
12	7.583639965175925E-5	0.0	0.0	2.5716502303909823	0.0
13	7.583639965175925E-5	0.0	0.0	2.677176580506405	0.0
14	7.583639965175925E-5	0.0	0.0	2.712402588144647	0.0
15	7.583639965175925E-5	0.0	0.0	2.7727683622674477	0.0
16	7.583639965175925E-5	0.0	0.0	2.921597296584025	0.0
17	7.583639965175925E-5	0.0	0.0	3.098296107772624	0.0
18	7.583639965175925E-5	0.0	0.0	3.4133205119260324	0.0
19	1.1375459947763888E-4	0.0	0.0	3.5158513242552107	0.0
20	1.1375459947763888E-4	0.0	0.0	3.6326014615190942	0.0
21	1.1375459947763888E-4	0.0	0.0	3.7890519540006733	0.0
22	1.1375459947763888E-4	0.0	0.0	3.9436065364909587	0.0
23	1.516727993035185E-4	0.0	0.0	4.143132103974738	0.0
24	4.5501839791055554E-4	0.0	0.0	4.2783484045538245	0.0
25	4.5501839791055554E-4	0.0	0.0	4.387021965254795	0.0
26	4.929365977364351E-4	0.0	0.0	4.499676936937483	0.0
27	4.929365977364351E-4	0.0	0.0	4.61161146282348	0.0
28	4.929365977364351E-4	0.0	0.0	4.720512532723406	0.0
29	4.929365977364351E-4	0.0	0.0	4.837907279384329	0.0
30	5.308547975623148E-4	0.0	0.0	5.034209799882909	0.0
31	5.308547975623148E-4	0.0	0.0	5.1886506277737166	0.0
32	5.308547975623148E-4	0.0	0.0	5.31597994278902	0.0
33	5.308547975623148E-4	0.0	0.0	5.440465392817383	0.0
34	5.308547975623148E-4	0.0	0.0	5.579852695377316	0.0
35	5.308547975623148E-4	0.0	0.0	5.812063751111003	0.0
36	5.308547975623148E-4	0.0	0.0	5.958807184437157	0.0
37	5.308547975623148E-4	0.0	0.0	6.10354095317254	0.0
38	5.308547975623148E-4	0.0	0.0	6.232993687378093	0.0
39	5.687729973881944E-4	0.0	0.0	6.375717791522704	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATGT	30	2.1665746E-6	45.000004	34
GCGATAC	95	0.0	45.000004	9
ACGTCCA	30	2.1665746E-6	45.000004	26
AATCCCG	25	3.892184E-5	45.0	28
GTACCAG	25	3.892184E-5	45.0	1
GTCGACG	90	0.0	45.0	1
TCCGATC	25	3.892184E-5	45.0	14
ACGTTAG	90	0.0	45.0	1
GTTTAGT	20	7.0349505E-4	45.0	44
AACCACG	35	1.2127202E-7	45.0	1
ACCGACA	25	3.892184E-5	45.0	27
CGATTCG	80	0.0	45.0	10
CGCGAAA	20	7.0349505E-4	45.0	26
CGTTCGA	25	3.892184E-5	45.0	14
CCTCGAC	45	3.8562575E-10	45.0	30
ACTAGTC	25	3.892184E-5	45.0	10
ATATCGA	20	7.0349505E-4	45.0	14
CCACTAG	35	1.2127202E-7	45.0	26
TGCGCAC	25	3.892184E-5	45.0	11
CGTAGAA	45	3.8562575E-10	45.0	34
>>END_MODULE