FastQCFastQC Report
Thu 26 May 2016
SRR1545252_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545252_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3405795
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT451811.326591882365204No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA75700.22226822225060525No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT306950.042.9255561
CGGTCTA2750.040.09090831
AGGGCGA31550.039.152146
GGGCGAT60400.038.22027
TAGGGAC34550.037.7062235
GCCCTAC21600.037.08333226
GGCGATA13300.036.8796968
AGGGATC29250.036.769236
ACGGGTA2450.036.7346955
CTACGGG5550.036.4864853
GTTTTTT374100.036.3332022
TAGGGCG12650.036.2845845
AAGGGAT51550.036.139675
CCGTCGA250.00210768836.05
GCCAGTA16500.035.86363625
TACGGGA11400.035.7236864
AGGGATT59750.035.6987426
TACGGGT2400.035.6250044
ATAGGGC14600.035.5993164
CTAATCC22400.035.4575911
Produced by FastQC (version 0.11.5)