##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545252_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3405795
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.100855747336524	31.0	31.0	33.0	28.0	34.0
2	31.395672082435965	31.0	31.0	34.0	30.0	34.0
3	31.436876558923835	31.0	31.0	34.0	30.0	34.0
4	35.2464329180118	37.0	35.0	37.0	33.0	37.0
5	35.12015197626398	37.0	35.0	37.0	32.0	37.0
6	35.11942292475032	37.0	35.0	37.0	32.0	37.0
7	35.79480444360274	37.0	35.0	37.0	35.0	37.0
8	35.87547606359161	37.0	35.0	37.0	35.0	37.0
9	37.47103246084982	39.0	37.0	39.0	35.0	39.0
10	36.689525353111385	39.0	35.0	39.0	32.0	39.0
11	36.40935758024191	38.0	35.0	39.0	32.0	39.0
12	36.057916874033815	38.0	35.0	39.0	31.0	39.0
13	35.89900713342993	38.0	35.0	39.0	30.0	39.0
14	36.75927558763813	39.0	35.0	41.0	31.0	41.0
15	36.998593867217494	39.0	35.0	41.0	31.0	41.0
16	36.79023076844026	39.0	35.0	41.0	31.0	41.0
17	36.83424751049314	38.0	35.0	41.0	31.0	41.0
18	36.767588477873744	38.0	35.0	40.0	31.0	41.0
19	36.63622267341399	38.0	35.0	40.0	31.0	41.0
20	36.46351820940485	38.0	35.0	40.0	31.0	41.0
21	36.369179883110995	38.0	35.0	40.0	30.0	41.0
22	36.28141388427665	38.0	35.0	40.0	30.0	41.0
23	36.170551662680815	38.0	35.0	40.0	30.0	41.0
24	35.9359952668907	38.0	34.0	40.0	30.0	41.0
25	35.892816801950794	37.0	34.0	40.0	30.0	41.0
26	35.7656256468754	37.0	34.0	40.0	30.0	41.0
27	35.735759198659935	37.0	34.0	40.0	29.0	41.0
28	35.6811190926054	37.0	34.0	40.0	29.0	41.0
29	35.60633714008036	37.0	34.0	40.0	29.0	41.0
30	35.42097748102866	37.0	34.0	40.0	28.0	41.0
31	35.253606573501926	37.0	34.0	40.0	27.0	41.0
32	35.0045763177173	37.0	34.0	40.0	25.0	41.0
33	34.68164907165581	37.0	33.0	40.0	24.0	41.0
34	34.34394084200605	37.0	33.0	40.0	23.0	41.0
35	34.08316560450643	37.0	33.0	40.0	22.0	41.0
36	33.89785732846516	37.0	33.0	40.0	21.0	41.0
37	33.722455696834366	37.0	33.0	40.0	20.0	41.0
38	33.69407994315571	37.0	33.0	40.0	20.0	41.0
39	33.6570985628906	37.0	33.0	40.0	20.0	41.0
40	33.544361595457154	36.0	32.0	40.0	18.0	41.0
41	33.4571699118708	36.0	32.0	40.0	18.0	41.0
42	33.36450344192765	36.0	32.0	40.0	18.0	41.0
43	33.221487494109304	36.0	32.0	40.0	18.0	41.0
44	33.14989070099639	36.0	32.0	40.0	18.0	41.0
45	33.075587050894136	36.0	31.0	40.0	18.0	41.0
46	32.967882095076185	35.0	31.0	40.0	18.0	41.0
47	32.97463264817759	35.0	31.0	40.0	18.0	41.0
48	32.88929779977949	35.0	31.0	40.0	18.0	41.0
49	32.84106031044147	35.0	31.0	39.0	18.0	41.0
50	32.67790515870744	35.0	31.0	39.0	18.0	41.0
51	32.536972718557635	35.0	31.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	1.0
11	4.0
12	10.0
13	12.0
14	40.0
15	159.0
16	464.0
17	1324.0
18	2966.0
19	5635.0
20	9550.0
21	14558.0
22	21302.0
23	30435.0
24	43088.0
25	61592.0
26	80406.0
27	90091.0
28	92293.0
29	97468.0
30	108139.0
31	126797.0
32	150893.0
33	187693.0
34	269534.0
35	343207.0
36	277751.0
37	336392.0
38	454066.0
39	599681.0
40	242.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.079031474296016	22.34468017012181	25.256951754289382	19.319336601292797
2	38.07466391840965	23.539349843428628	26.19925744209502	12.186728796066703
3	28.471972035897643	24.177350662620622	34.29818882228672	13.052488479195018
4	24.857309380041958	26.876309349212153	33.981640116331135	14.284741154414753
5	21.939106728385003	30.687372551783064	33.08246679556462	14.291053924267317
6	20.221475455804004	39.61950146735197	30.64829210213768	9.510730974706346
7	82.00895826084658	4.14913404946569	12.115438539313143	1.7264691503745822
8	82.70074975152644	3.4161774270030936	12.079352985132694	1.8037198363377713
9	77.8984642352226	5.535154053605693	13.656429702903432	2.9099520082682604
10	38.35803388048899	31.060442569209247	19.5108337407272	11.070689809574564
11	29.043967707980073	24.57893091040418	30.95732420771068	15.419777173905064
12	27.303962804572794	22.708853586313914	34.482257446499275	15.504926162614016
13	21.82033856999614	28.308045551772786	33.84187832796748	16.029737550263594
14	18.13165501740416	31.13472772142774	34.58596304240273	16.14765421876537
15	17.275232361313584	25.08060526250112	41.76552023829972	15.878642137885574
16	20.65177146598665	22.775445967828365	39.01670535073309	17.5560772154519
17	20.063920464972202	22.143611109887708	34.03819078952198	23.754277635618116
18	21.24784374866955	23.55344346914597	36.99688325339605	18.201829528788434
19	26.15081060369165	24.1623468235757	31.47147729091152	18.215365281821132
20	26.213292344371876	24.37624695555663	31.789552806319815	17.620907893751678
21	23.242767107239278	25.719751188782645	33.749917420161815	17.28756428381626
22	21.736099794614766	23.579428591562323	32.3440782548568	22.340393358966114
23	19.631980198455867	27.18727932832129	32.71829337937251	20.462447093850333
24	19.228990588100576	25.2390704666605	37.88660210024385	17.645336844995075
25	20.28674655990745	25.752577592015964	35.47430188839904	18.48637395967755
26	18.804889900889513	29.274809552542063	32.243015213775344	19.677285332793076
27	18.58960976805709	28.244741682925717	34.192985778650794	18.97266277036639
28	18.41693349129939	26.544287016687733	36.774497584264466	18.264281907748412
29	19.322537028799445	25.224066627615578	35.78662250663942	19.66677383694556
30	20.3825244913449	27.187161881440307	34.65510989357845	17.775203733636346
31	24.19614216357708	26.373724783787633	31.477584528722367	17.95254852391292
32	25.218605347650108	25.71916395437776	31.189399244522935	17.872831453449194
33	24.950826459020583	26.52508445164785	29.65175531704051	18.87233377229105
34	20.653239551998873	27.314621109021537	31.20813202203891	20.824007316940683
35	20.995685295210077	27.31873174985576	31.975794197830464	19.709788757103702
36	25.371462463242793	28.05926369614143	28.380715809377836	18.188558031237932
37	21.111487919854248	31.147089005650663	29.732411962552064	18.00901111194303
38	21.100535998203064	30.9542999505255	28.750908378220064	19.194255673051373
39	20.846292862606234	30.2309152488626	28.609619780403694	20.31317210812747
40	22.692528469857994	27.089592885067947	30.213180769834942	20.004697875239113
41	19.67329213883983	26.78241056787035	30.28831741194053	23.25597988134929
42	20.733808112349685	27.637923010633347	29.047931540213078	22.580337336803886
43	20.68539063566656	26.942079602559755	30.135078593984666	22.23745116778902
44	20.265253780688504	28.623742767841282	30.88315650237316	20.227846949097053
45	19.692641512480932	31.600580774826437	28.38926006996898	20.31751764272365
46	21.388280856598826	30.113527091325228	29.10198646718314	19.396205584892808
47	20.77702856454954	28.73575773057392	29.590888470973738	20.896325233902804
48	20.457661133450486	28.572653374615914	31.14700092048993	19.822684571443673
49	21.119151328838054	27.62952555864343	31.574213950046904	19.677109162471613
50	20.0172353297835	30.754111741898733	29.938560600388453	19.290092327929308
51	19.64184573645801	30.448808574796782	29.5388301409803	20.37051554776491
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	8494.0
1	7704.5
2	6915.0
3	40101.0
4	73287.0
5	52529.5
6	31772.0
7	31582.0
8	31392.0
9	32372.5
10	33353.0
11	33170.5
12	32988.0
13	32664.0
14	32340.0
15	31280.5
16	30221.0
17	28974.0
18	27727.0
19	26647.0
20	25567.0
21	25526.5
22	25486.0
23	26813.5
24	28141.0
25	31081.0
26	38275.5
27	42530.0
28	47395.5
29	52261.0
30	64096.5
31	75932.0
32	85954.5
33	95977.0
34	105444.5
35	114912.0
36	125578.0
37	136244.0
38	150202.0
39	164160.0
40	183736.5
41	203313.0
42	224321.0
43	245329.0
44	250277.0
45	255225.0
46	253624.5
47	252024.0
48	238674.0
49	225324.0
50	210453.0
51	195582.0
52	181845.0
53	168108.0
54	155608.5
55	143109.0
56	135745.5
57	128382.0
58	122303.0
59	116224.0
60	105818.0
61	95412.0
62	87064.0
63	78716.0
64	68973.0
65	59230.0
66	50388.0
67	41546.0
68	37000.5
69	32455.0
70	27917.0
71	23379.0
72	20149.5
73	16920.0
74	13542.0
75	7989.0
76	5814.0
77	4510.0
78	3206.0
79	2329.5
80	1453.0
81	1126.0
82	799.0
83	493.5
84	188.0
85	143.0
86	98.0
87	70.0
88	42.0
89	34.0
90	26.0
91	15.0
92	4.0
93	2.0
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3405795.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.157192234601652
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.15378930270043	18.098223227451886
2	6.009020013219015	2.7830406317536256
3	2.5008921718986046	1.7374092234799927
4	1.6101736670324003	1.4914840455425107
5	1.133390723064341	1.3123073425457554
6	0.8881282388317622	1.2339933813363193
7	0.7039631764615489	1.1411267422380627
8	0.5820177868268531	1.0782318218805476
9	0.4913727506598989	1.0240931921288638
>10	6.198244516918377	33.88198125964512
>50	1.3382399689102709	21.57006810462395
>100	0.38503870876841195	11.879492453292327
>500	0.0033853032366204195	0.5193308789925227
>1k	0.0020832635302279086	0.6586914992144959
>5k	1.3020397063924429E-4	0.22824748919956667
>10k+	1.3020397063924429E-4	1.3622787066744546
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	45181	1.326591882365204	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7570	0.22226822225060525	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.9361720244465684E-5	0.0	0.0	0.024164695761195256	0.0
2	2.9361720244465684E-5	0.0	0.0	0.08018685798763578	0.0
3	2.9361720244465684E-5	0.0	0.0	0.13823497891094444	0.0
4	2.9361720244465684E-5	0.0	0.0	0.18941245729704811	0.0
5	5.872344048893137E-5	0.0	0.0	0.3284402026545931	0.0
6	5.872344048893137E-5	0.0	0.0	0.5705275860702127	0.0
7	5.872344048893137E-5	0.0	0.0	0.7351000280404428	0.0
8	5.872344048893137E-5	0.0	0.0	1.0346776596947262	0.0
9	5.872344048893137E-5	0.0	0.0	1.2070015958094953	0.0
10	5.872344048893137E-5	0.0	0.0	1.430620457191346	0.0
11	5.872344048893137E-5	0.0	0.0	1.5903482153212392	0.0
12	5.872344048893137E-5	0.0	0.0	1.726968299618738	0.0
13	8.808516073339705E-5	0.0	0.0	1.7974070664852113	0.0
14	8.808516073339705E-5	0.0	0.0	1.8432113500665777	0.0
15	1.4680860122232841E-4	0.0	0.0	1.8811760543426719	0.0
16	1.4680860122232841E-4	0.0	0.0	1.9468288608092972	0.0
17	1.4680860122232841E-4	0.0	0.0	2.0268689101957107	0.0
18	1.4680860122232841E-4	0.0	0.0	2.154445584657914	0.0
19	1.4680860122232841E-4	0.0	0.0	2.2088528522709088	0.0
20	1.4680860122232841E-4	0.0	0.0	2.2725677852013995	0.0
21	1.4680860122232841E-4	0.0	0.0	2.338954634674136	0.0
22	1.4680860122232841E-4	0.0	0.0	2.416381490958792	0.0
23	1.4680860122232841E-4	0.0	0.0	2.5096343144552153	0.0
24	1.4680860122232841E-4	0.0	0.0	2.587442873103049	0.0
25	1.4680860122232841E-4	0.0	0.0	2.659173555660279	0.0
26	1.4680860122232841E-4	0.0	0.0	2.728584662318196	0.0
27	1.4680860122232841E-4	0.0	0.0	2.8150842901583917	0.0
28	1.761703214667941E-4	0.0	0.0	2.8950362543840717	0.0
29	1.761703214667941E-4	0.0	0.0	2.9879073755173167	0.0
30	1.761703214667941E-4	0.0	0.0	3.1196534142542345	0.0
31	2.0553204171125977E-4	0.0	0.0	3.2177509215909943	0.0
32	2.0553204171125977E-4	0.0	0.0	3.3181973665473112	0.0
33	2.0553204171125977E-4	0.0	0.0	3.4181446622594724	0.0
34	2.0553204171125977E-4	0.0	0.0	3.5277813256523074	0.0
35	2.0553204171125977E-4	0.0	0.0	3.660848641800226	0.0
36	2.0553204171125977E-4	0.0	0.0	3.7650533869478346	0.0
37	2.0553204171125977E-4	0.0	0.0	3.8824415444852085	0.0
38	2.0553204171125977E-4	0.0	0.0	4.013248008174303	0.0
39	2.0553204171125977E-4	0.0	0.0	4.230319205941638	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	30695	0.0	42.925556	1
CGGTCTA	275	0.0	40.090908	31
AGGGCGA	3155	0.0	39.15214	6
GGGCGAT	6040	0.0	38.2202	7
TAGGGAC	3455	0.0	37.706223	5
GCCCTAC	2160	0.0	37.083332	26
GGCGATA	1330	0.0	36.879696	8
AGGGATC	2925	0.0	36.76923	6
ACGGGTA	245	0.0	36.734695	5
CTACGGG	555	0.0	36.486485	3
GTTTTTT	37410	0.0	36.333202	2
TAGGGCG	1265	0.0	36.284584	5
AAGGGAT	5155	0.0	36.13967	5
CCGTCGA	25	0.002107688	36.0	5
GCCAGTA	1650	0.0	35.863636	25
TACGGGA	1140	0.0	35.723686	4
AGGGATT	5975	0.0	35.698742	6
TACGGGT	240	0.0	35.625004	4
ATAGGGC	1460	0.0	35.599316	4
CTAATCC	2240	0.0	35.45759	11
>>END_MODULE