##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545251_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3399823
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.24602163112609	31.0	31.0	34.0	30.0	34.0
2	31.492957133356647	31.0	31.0	34.0	30.0	34.0
3	31.567243647684013	31.0	31.0	34.0	30.0	34.0
4	35.30802132934568	37.0	35.0	37.0	33.0	37.0
5	35.15879032526105	37.0	35.0	37.0	32.0	37.0
6	35.14332893212382	37.0	35.0	37.0	32.0	37.0
7	35.84491810308948	37.0	35.0	37.0	35.0	37.0
8	35.97169529119604	37.0	35.0	37.0	35.0	37.0
9	37.669677509682124	39.0	37.0	39.0	35.0	39.0
10	36.8691128920535	39.0	37.0	39.0	32.0	39.0
11	36.42133281644368	38.0	35.0	39.0	32.0	39.0
12	35.022311161492816	35.0	34.0	39.0	30.0	39.0
13	34.299677659689934	35.0	33.0	39.0	25.0	39.0
14	34.94457093795765	35.0	33.0	40.0	25.0	41.0
15	35.58452866516875	35.0	33.0	40.0	30.0	41.0
16	35.66397074200628	35.0	34.0	40.0	31.0	41.0
17	35.782846930560794	35.0	34.0	40.0	31.0	41.0
18	35.89644902102256	36.0	35.0	40.0	31.0	41.0
19	35.80007341558664	36.0	35.0	40.0	31.0	41.0
20	35.59365472849616	35.0	34.0	40.0	31.0	41.0
21	35.41621666775006	35.0	34.0	40.0	30.0	41.0
22	35.30261457728829	35.0	34.0	40.0	30.0	41.0
23	35.29956206543694	35.0	34.0	40.0	30.0	41.0
24	35.14796829129046	35.0	34.0	39.0	30.0	41.0
25	35.16826434787929	35.0	34.0	39.0	30.0	41.0
26	35.03668926294104	35.0	34.0	39.0	29.0	41.0
27	34.929605747122714	35.0	34.0	39.0	29.0	41.0
28	35.067263501658765	35.0	34.0	39.0	29.0	41.0
29	35.118427635791626	36.0	34.0	39.0	29.0	41.0
30	35.06017725040392	36.0	34.0	39.0	29.0	41.0
31	34.864280287532615	35.0	34.0	39.0	29.0	41.0
32	34.65231895895757	35.0	34.0	39.0	29.0	41.0
33	34.44116679015349	35.0	33.0	39.0	27.0	41.0
34	34.322640031554585	35.0	34.0	39.0	27.0	41.0
35	34.12728427332835	35.0	33.0	39.0	26.0	41.0
36	33.84444484315801	35.0	33.0	39.0	24.0	41.0
37	33.6731241596989	35.0	33.0	39.0	23.0	41.0
38	33.83181771521635	35.0	33.0	39.0	24.0	41.0
39	33.86450765231014	35.0	33.0	39.0	24.0	41.0
40	33.679277127073966	35.0	33.0	39.0	23.0	41.0
41	33.7933886558212	35.0	33.0	39.0	24.0	41.0
42	33.74812218165475	35.0	33.0	39.0	24.0	41.0
43	33.59438829609659	35.0	33.0	39.0	23.0	41.0
44	33.57647177514829	35.0	33.0	38.0	23.0	41.0
45	33.50057547113482	35.0	33.0	38.0	23.0	40.0
46	33.47994792670089	35.0	33.0	38.0	23.0	40.0
47	33.51805255744196	35.0	33.0	38.0	23.0	40.0
48	33.57284099789901	35.0	33.0	38.0	23.0	40.0
49	33.61510996307749	35.0	33.0	38.0	24.0	40.0
50	33.343575827329836	35.0	33.0	38.0	24.0	40.0
51	33.147666216741285	35.0	32.0	38.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	3.0
12	3.0
13	9.0
14	26.0
15	85.0
16	245.0
17	811.0
18	1926.0
19	3760.0
20	6603.0
21	10844.0
22	16315.0
23	24062.0
24	34487.0
25	47539.0
26	61095.0
27	71900.0
28	80083.0
29	93343.0
30	112029.0
31	140190.0
32	178359.0
33	236576.0
34	423520.0
35	658884.0
36	201958.0
37	229660.0
38	308074.0
39	457035.0
40	398.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.080036519548226	25.80957891043151	30.159128872297174	13.951255697723086
2	28.88579787830131	27.091351520358558	31.43257751947675	12.59027308186338
3	26.62476840706119	26.442611865382403	33.42715194290997	13.505467784646436
4	24.36468016129075	28.374918341337178	33.16816787226865	14.092233625103425
5	22.789656990966883	32.85229848730361	30.758748323074464	13.599296198655047
6	20.17516794256642	40.98822203391176	28.38121278666566	10.455397236856154
7	86.2290772196082	5.432635757802686	6.646551894025071	1.691735128564046
8	89.16020039866781	3.249227974515144	6.058109495700218	1.5324621311168258
9	86.02306649493224	4.572002718965075	7.3612655717665305	2.043665214336158
10	61.90422266100324	20.39035561557175	10.836181765933109	6.869239957491905
11	56.99249637407594	15.916652131596262	18.023085319441627	9.067766174886163
12	50.916327114676264	20.396002968389826	19.576725023626228	9.11094489330768
13	18.423459103606277	53.266331806096964	19.439864957675738	8.870344132621021
14	10.687732861387197	53.12241254912388	25.31752388286096	10.872330706627963
15	8.440968838671896	22.868719930419907	58.72561601000993	9.964695220898264
16	10.098437477480445	16.21063802439127	55.88605642117251	17.80486807695577
17	10.590874877898056	16.077542860319493	29.875143500117506	43.45643876166495
18	17.034092657176565	21.84622552409346	42.74957843393612	18.370103384793858
19	32.12781959531423	21.734807959120225	25.85322824158787	20.28414420397768
20	33.52780424157375	21.380171850122785	27.306480366772035	17.785543541531425
21	18.243302666050557	33.21990585980505	29.603452885635516	18.93333858850887
22	20.104928991891637	24.972711814703295	22.90325113983875	32.01910805356632
23	14.113470024763053	35.346928354799644	24.361709418402075	26.177892202035224
24	13.83613205746299	22.42752049150794	49.55481505948986	14.181532391539205
25	11.22361369988967	25.661188832477457	45.333389414684234	17.781808052948637
26	11.218378133214582	41.18493815707465	28.42892115266001	19.167762557050764
27	12.338230549060937	45.35080208587329	27.919806413451525	14.391160951614246
28	9.94407650045311	32.75535226392668	43.65174304662331	13.648828188996898
29	10.025933703019245	25.300522997814884	42.76590281317586	21.907640485990008
30	14.393131642441388	39.224424330325434	31.344014085439152	15.038429941794028
31	32.19123466133384	29.36041082138688	24.106254943272045	14.342099574007236
32	34.46388238446531	24.705903807345265	27.36345392098353	13.466759887205892
33	32.89306531545907	27.370483698710196	22.688651732751968	17.047799253078765
34	18.33986651658042	25.98649988543521	27.009759037455773	28.66387456052859
35	18.097942157577027	24.77511329266259	35.08150277235021	22.045441777410176
36	36.18326601120117	24.55177813668535	24.678049416101956	14.586906436011521
37	17.195630478410198	39.035061531144414	29.817169893844476	13.95213809660091
38	16.570039087328958	40.12708896904339	23.81041601283361	19.492455930794044
39	18.029791550913092	38.39370461344605	27.188150677255845	16.388353158385012
40	26.962844830451466	24.507746432681937	24.83288100586413	23.69652773100247
41	13.74392137473039	23.806798177434533	27.624202789380508	34.825077658454575
42	17.727834654921743	23.347450734935318	25.629304819692084	33.29540979045086
43	18.202977037334005	23.342715194290996	28.391448613648418	30.062859154726585
44	14.844508081744257	30.23007374207422	33.61842660632627	21.306991569855256
45	12.736398336031025	47.07062691204807	21.926229689016164	18.266745062904747
46	20.13622473875846	39.515410066935836	24.553278214777652	15.795086979528053
47	17.53923660143484	29.494682517295757	26.21121746632104	26.754863414948364
48	20.052749804916314	24.793702495688745	36.746677694691755	18.406870004703187
49	20.725931908808192	23.34462705852628	37.880707319175144	18.04873371349038
50	18.145268150724316	40.73988557639618	25.108307108928905	16.006539163950595
51	15.314326657593647	39.29010422013146	23.798827174238188	21.59674194803671
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	15005.0
1	12351.5
2	9698.0
3	18035.0
4	26372.0
5	18681.0
6	10990.0
7	11991.0
8	12992.0
9	14734.0
10	16476.0
11	18293.5
12	20111.0
13	19542.5
14	18974.0
15	17912.0
16	16850.0
17	15630.5
18	14411.0
19	13158.5
20	11906.0
21	13275.0
22	14644.0
23	15226.5
24	15809.0
25	18865.0
26	24297.0
27	26673.0
28	35631.5
29	44590.0
30	50328.0
31	56066.0
32	64877.0
33	73688.0
34	87957.5
35	102227.0
36	112520.5
37	122814.0
38	150396.5
39	177979.0
40	252822.5
41	327666.0
42	405957.0
43	484248.0
44	485680.0
45	487112.0
46	448598.5
47	410085.0
48	340272.5
49	270460.0
50	225952.5
51	181445.0
52	156979.0
53	132513.0
54	109796.0
55	87079.0
56	71281.0
57	55483.0
58	47867.0
59	40251.0
60	35647.0
61	31043.0
62	27032.0
63	23021.0
64	18459.5
65	13898.0
66	11105.5
67	8313.0
68	6949.0
69	5585.0
70	4746.5
71	3908.0
72	3664.0
73	3420.0
74	2665.5
75	1467.0
76	1023.0
77	877.5
78	732.0
79	457.5
80	183.0
81	153.5
82	124.0
83	113.5
84	103.0
85	59.0
86	15.0
87	8.5
88	2.0
89	2.0
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3399823.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.882313738422054
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.52698518344924	11.388986029556678
2	9.74728742715035	2.901243791788564
3	3.8026659711451036	1.6977740407500843
4	2.022380611020506	1.2039081100683544
5	1.277133215864413	0.9503348602127047
6	0.8370833891198337	0.7474642573261797
7	0.5864850905531668	0.6109778584363368
8	0.42634553857449325	0.5076006452833705
9	0.3351514695147825	0.44890463872909686
>10	2.375132821174349	7.514777532626014
>50	0.6387867111790028	6.918819969257307
>100	1.1733983404768746	38.53468793087046
>500	0.23271134851665723	21.645581646494556
>1k	0.01783778618585831	3.6754132153756918
>5k	2.0503202512480814E-4	0.297384661085341
>10k+	4.100640502496163E-4	0.9561408121392531
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17655	0.519291739599385	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13680	0.4023738882877138	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	9746	0.2866619821090686	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4860	0.14294861820747728	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	3994	0.11747670393429306	No Hit
AGGAAGGGATAGCCTTCTGTAATTCCATTCCCCAAAATTTGACTCCCTTTT	3694	0.10865271515605371	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.050237909444109295	0.0
2	0.0	0.0	0.0	0.1933335941312239	0.0
3	0.0	0.0	0.0	0.2833382796692651	0.0
4	0.0	0.0	0.0	0.4052269779926779	0.0
5	0.0	0.0	0.0	0.7503625924055458	0.0
6	0.0	0.0	0.0	1.023759178051328	0.0
7	0.0	0.0	0.0	1.2217106596431637	0.0
8	0.0	0.0	0.0	1.6807639691831016	0.0
9	0.0	0.0	0.0	1.8647735485053192	0.0
10	2.941329592746446E-5	0.0	0.0	2.198290911026839	0.0
11	2.941329592746446E-5	0.0	0.0	2.541720554275914	0.0
12	2.941329592746446E-5	0.0	0.0	2.844501022553233	0.0
13	2.941329592746446E-5	0.0	0.0	2.959124636782562	0.0
14	2.941329592746446E-5	0.0	0.0	2.9949500312222135	0.0
15	2.941329592746446E-5	0.0	0.0	3.0551590479857333	0.0
16	2.941329592746446E-5	0.0	0.0	3.212520181197668	0.0
17	2.941329592746446E-5	0.0	0.0	3.406442041247441	0.0
18	2.941329592746446E-5	0.0	0.0	3.715075755414326	0.0
19	2.941329592746446E-5	0.0	0.0	3.8432294857702884	0.0
20	5.882659185492892E-5	0.0	0.0	3.976177583362428	0.0
21	5.882659185492892E-5	0.0	0.0	4.157275246387827	0.0
22	5.882659185492892E-5	0.0	0.0	4.339314134882904	0.0
23	8.823988778239338E-5	0.0	0.0	4.562178678125302	0.0
24	8.823988778239338E-5	0.0	0.0	4.716686721632273	0.0
25	8.823988778239338E-5	0.0	0.0	4.843928639814484	0.0
26	8.823988778239338E-5	0.0	0.0	4.982641743408407	0.0
27	8.823988778239338E-5	0.0	0.0	5.111942592305541	0.0
28	8.823988778239338E-5	0.0	0.0	5.240861068355617	0.0
29	8.823988778239338E-5	0.0	0.0	5.385398004543178	0.0
30	8.823988778239338E-5	0.0	0.0	5.602056342344881	0.0
31	1.470664796373223E-4	0.0	0.0	5.7978900666299396	0.0
32	1.470664796373223E-4	0.0	0.0	5.9595749543432115	0.0
33	1.470664796373223E-4	0.0	0.0	6.117730246545188	0.0
34	1.470664796373223E-4	0.0	0.0	6.292533464242109	0.0
35	1.470664796373223E-4	0.0	0.0	6.55690016803816	0.0
36	1.470664796373223E-4	0.0	0.0	6.749439603179342	0.0
37	1.470664796373223E-4	0.0	0.0	6.929801933806554	0.0
38	1.470664796373223E-4	0.0	0.0	7.11510569814958	0.0
39	1.470664796373223E-4	0.0	0.0	7.302880179350513	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGCGT	20	7.0352375E-4	45.000004	18
CTACGGC	20	7.0352375E-4	45.000004	12
GCTTACG	40	6.8212103E-9	45.000004	1
AGGTGCG	20	7.0352375E-4	45.000004	1
CCGGATG	25	3.892424E-5	45.0	23
CGCGATA	35	1.2128658E-7	45.0	11
TAAGTCG	90	0.0	45.0	1
GTGTCGC	25	3.892424E-5	45.0	45
ACCGTAG	55	1.8189894E-12	45.0	2
GAACCGT	25	3.892424E-5	45.0	45
CGCAATA	25	3.892424E-5	45.0	41
ATACGGT	55	1.8189894E-12	45.0	29
TACCGGA	35	1.2128658E-7	45.0	22
TCACGTA	25	3.892424E-5	45.0	41
CGCTGTA	30	2.166762E-6	44.999996	16
CCGTCAC	30	2.166762E-6	44.999996	29
GCGTGCC	30	2.166762E-6	44.999996	35
ACGCGAG	335	0.0	42.985077	1
TATTACG	105	0.0	42.857143	1
GACAGCG	100	0.0	42.75	1
>>END_MODULE