FastQCFastQC Report
Thu 26 May 2016
SRR1545250_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545250_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1440659
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60800.42202908530054645No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC32580.22614650656400995No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28880.20046381551775955No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG23460.16284214376892797No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTA253.8912993E-545.026
CGTAGGA207.0338824E-445.035
TATTCGA207.0338824E-445.038
ACGTCCA253.8912993E-545.012
TCATACG253.8912993E-545.01
TTATTCG207.0338824E-445.037
TATCGGA253.8912993E-545.036
GCGTATT207.0338824E-445.022
GCGATCG302.165887E-644.9999969
CGGTCTA1150.044.99999631
TCGATGG1200.043.1249962
TATAGCG1500.042.0000041
AATACGG700.041.7857172
ACGGGAT4250.041.2941175
TACGGGA2900.040.344834
ACGGGTA950.040.2631575
ACGGGAC2850.040.2631575
TCGCCTA451.9288564E-840.033
AGGGTAC3900.039.8076936
CTCACGA1300.039.8076924