##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545250_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1440659
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.20562673054484	31.0	31.0	33.0	30.0	34.0
2	31.46181712674547	31.0	31.0	34.0	30.0	34.0
3	31.549752578507476	31.0	31.0	34.0	30.0	34.0
4	35.312550714638235	37.0	35.0	37.0	33.0	37.0
5	35.1622174296624	37.0	35.0	37.0	32.0	37.0
6	35.15416417070244	37.0	35.0	37.0	32.0	37.0
7	35.82506894414292	37.0	35.0	37.0	35.0	37.0
8	35.90992039059903	37.0	35.0	37.0	35.0	37.0
9	37.530989637381225	39.0	37.0	39.0	35.0	39.0
10	36.75979881429262	39.0	37.0	39.0	32.0	39.0
11	36.40339386350274	38.0	35.0	39.0	32.0	39.0
12	35.455140321200226	37.0	35.0	39.0	30.0	39.0
13	35.0035761411965	37.0	34.0	39.0	29.0	39.0
14	35.77112279866367	38.0	34.0	40.0	27.0	41.0
15	36.206482588870784	38.0	35.0	40.0	30.0	41.0
16	36.15280784696448	38.0	35.0	40.0	31.0	41.0
17	36.23026753728676	37.0	34.0	40.0	31.0	41.0
18	36.285338168157764	37.0	35.0	40.0	31.0	41.0
19	36.21933018153498	37.0	35.0	40.0	31.0	41.0
20	36.07248071889323	37.0	34.0	40.0	31.0	41.0
21	35.948556875707574	37.0	34.0	40.0	30.0	41.0
22	35.81921606709152	36.0	34.0	40.0	30.0	41.0
23	35.78274248104513	36.0	34.0	40.0	30.0	41.0
24	35.57950077013367	36.0	34.0	40.0	30.0	41.0
25	35.56739103424197	36.0	34.0	40.0	30.0	41.0
26	35.40714284226871	36.0	34.0	40.0	29.0	41.0
27	35.35683045050911	36.0	34.0	40.0	29.0	41.0
28	35.369725937921466	36.0	34.0	40.0	29.0	41.0
29	35.39315549342349	36.0	34.0	40.0	29.0	41.0
30	35.313590516562215	36.0	34.0	40.0	29.0	41.0
31	35.19371967967437	36.0	34.0	40.0	29.0	41.0
32	35.03222275361484	35.0	34.0	40.0	29.0	41.0
33	34.84955634886535	35.0	34.0	40.0	27.0	41.0
34	34.6850732893766	35.0	34.0	40.0	26.0	41.0
35	34.51598053390844	35.0	33.0	40.0	26.0	41.0
36	34.30173552520062	35.0	33.0	40.0	24.0	41.0
37	34.076175555769964	35.0	33.0	40.0	23.0	41.0
38	34.1617676355057	35.0	33.0	40.0	24.0	41.0
39	34.17694610591403	35.0	33.0	40.0	24.0	41.0
40	33.97795939219482	35.0	33.0	40.0	23.0	41.0
41	33.998431273465826	35.0	33.0	40.0	23.0	41.0
42	33.94269497500797	35.0	33.0	40.0	23.0	41.0
43	33.798864963881115	35.0	33.0	39.0	23.0	41.0
44	33.741087932675256	35.0	33.0	39.0	23.0	41.0
45	33.68199691946533	35.0	33.0	39.0	23.0	41.0
46	33.599407632201654	35.0	33.0	39.0	23.0	41.0
47	33.63180947052703	35.0	33.0	39.0	23.0	41.0
48	33.58535642369221	35.0	33.0	39.0	23.0	41.0
49	33.56496575525506	35.0	33.0	39.0	24.0	41.0
50	33.35318003774661	35.0	32.0	39.0	23.0	40.0
51	33.204103122251695	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	1.0
14	15.0
15	50.0
16	140.0
17	390.0
18	889.0
19	1759.0
20	2984.0
21	4892.0
22	7134.0
23	10096.0
24	14346.0
25	19483.0
26	25223.0
27	29988.0
28	33767.0
29	39498.0
30	46999.0
31	57259.0
32	70727.0
33	92317.0
34	153264.0
35	215237.0
36	103138.0
37	121770.0
38	163630.0
39	225498.0
40	162.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.29830306824863	23.653550215561072	28.545616971122246	14.502529745068054
2	30.80777616354738	26.375984879142116	29.224472966885294	13.591765990425214
3	28.391381999487734	25.971170138110406	32.078028180159215	13.559419682242641
4	26.235146554458762	27.538786069430728	30.73544815254686	15.490619223563662
5	23.637446474148287	32.27745080549943	29.618806393462993	14.46629632688929
6	21.896160021212516	41.575695567098116	25.99588105165761	10.532263360031763
7	86.19124997657323	5.1703421836812185	6.7559359987339125	1.8824718410116483
8	87.73623737470145	4.0530062978123205	6.631687304212863	1.5790690232733768
9	83.00888690522879	5.66580988283834	8.236716669246505	3.0885865426863677
10	52.952711224516	22.960534033383333	13.579271708294607	10.507483033806057
11	47.22255578870503	20.97401258729512	19.216483567589552	12.586948056410296
12	42.067623219651566	22.002986133429214	23.200285424934005	12.729105221985215
13	21.582275889020234	40.817639705162705	23.45857000164508	14.141514404171978
14	15.000079824580279	43.30351596040423	26.78281258785042	14.913591627165069
15	14.14172264220749	23.869007169635562	48.27283902713966	13.716431161017283
16	16.54229071556836	18.266987538341827	46.802609083759585	18.388112662330226
17	16.277550759756473	18.737466673237734	29.60166146187266	35.383321105133135
18	20.325004043288523	24.23828261927354	35.81860801202783	19.618105325410106
19	30.77418042715174	23.36701467869912	26.472121438869294	19.38668345527984
20	32.382402775396535	22.51247519364402	26.278460065844868	18.826661965114578
21	22.5593287516338	28.98604041622618	28.67680693349363	19.777823898646382
22	22.134870222585636	25.60585121114712	24.129929428129763	28.129349138137478
23	19.815862046466233	31.038087430821587	24.951081414824746	24.19496910788743
24	18.66777634402034	24.04191415178748	40.210209355579636	17.080100148612544
25	17.67031615392678	25.63188096558589	36.62566922498662	20.072133655500714
26	16.844860581164593	34.770476566626805	27.539133132823242	20.845529719385365
27	17.963654133282063	36.812875218910236	27.427239895075793	17.79623075273191
28	15.495408698380395	30.880173587226402	37.01195078085793	16.612466933535277
29	15.788469027021662	26.3078910415303	35.92814121870616	21.975498712741878
30	18.795356847109552	32.66331588529972	30.423438162674167	18.11788910491657
31	29.437500477212165	28.22305625411704	24.53488299451848	17.804560274152315
32	31.546812951572857	25.04860622812199	27.109676890922835	16.29490392938232
33	30.09122908335699	27.318192577146988	23.55588657690682	19.034691762589205
34	20.368178729317624	27.136956073574662	27.472427548781496	25.022437648326218
35	21.672720609110137	25.4657070132488	30.945074441627064	21.916497936014004
36	32.175830644170475	26.168718621131028	24.25056866336864	17.404882071329858
37	20.808116285672043	35.214023582263394	27.554403922093982	16.423456209970578
38	20.392473166793806	35.62112894168571	23.41005053937122	20.57634735214926
39	21.895604719784487	32.97657530338547	25.62264907934494	19.505170897485108
40	24.948999034469644	25.007791573162002	26.96495145624329	23.078257936125066
41	17.481721906433098	25.481741341983078	27.70620944998088	29.33032730160295
42	20.456402243695422	25.627924442911194	25.25469247059852	28.66098084279486
43	21.04689589972367	24.972807583196303	27.7293238719225	26.25097264515753
44	18.361597019141936	30.08248308586556	30.433503001057154	21.122416893935345
45	17.27799569502568	38.75899848610948	23.575669190280284	20.387336628584556
46	21.089168220932226	34.55633845344387	25.475633026274778	18.878860299349117
47	21.603030279892742	28.786062489457947	26.37952492574579	23.23138230490352
48	22.31978559811864	25.166954844970252	32.37650269772375	20.136756859187358
49	21.708884614610398	25.722048034961777	32.89189183561134	19.677175514816483
50	19.316090761241902	35.83457292808361	26.443106939254886	18.40622937141961
51	18.73781373663025	33.7536502392308	25.059781669361037	22.44875435477792
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3394.0
1	3600.0
2	3806.0
3	7729.0
4	11652.0
5	8736.0
6	5820.0
7	5800.0
8	5780.0
9	6085.5
10	6391.0
11	6642.5
12	6894.0
13	6930.5
14	6967.0
15	6988.5
16	7010.0
17	6270.0
18	5530.0
19	5347.5
20	5165.0
21	5478.0
22	5791.0
23	6011.5
24	6232.0
25	8143.0
26	11936.0
27	13818.0
28	17301.0
29	20784.0
30	24330.0
31	27876.0
32	29990.0
33	32104.0
34	36734.5
35	41365.0
36	44858.0
37	48351.0
38	57178.5
39	66006.0
40	87570.0
41	109134.0
42	126139.0
43	143144.0
44	144784.5
45	146425.0
46	138947.5
47	131470.0
48	118433.0
49	105396.0
50	95176.5
51	84957.0
52	78231.0
53	71505.0
54	64510.5
55	57516.0
56	53293.5
57	49071.0
58	47415.0
59	45759.0
60	45740.5
61	45722.0
62	39667.0
63	33612.0
64	28013.5
65	22415.0
66	19826.5
67	17238.0
68	14600.5
69	11963.0
70	10984.5
71	10006.0
72	7901.0
73	5796.0
74	4897.5
75	3130.0
76	2261.0
77	1787.0
78	1313.0
79	1020.0
80	727.0
81	485.5
82	244.0
83	199.0
84	154.0
85	91.0
86	28.0
87	16.0
88	4.0
89	5.0
90	6.0
91	3.0
92	0.0
93	2.0
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1440659.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.226241698767655
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.18316726709658	15.031684706523787
2	6.908683561461122	2.656560399459089
3	2.5846948616594445	1.490819043834819
4	1.3943385762449305	1.0723156190720242
5	0.9167712393770635	0.8813032715371101
6	0.6716108599061182	0.7747531632043329
7	0.5138058064789726	0.6914988235116476
8	0.4239265618022408	0.6520411631789957
9	0.37437805970973903	0.64780947564256
>10	5.622040989608215	26.689399084475628
>50	1.4022546406895027	18.691729150399524
>100	0.997614654923591	28.936633375331848
>500	0.004475280694984329	0.5758416284753669
>1k	0.0018647002895768037	0.7716607652411778
>5k	3.729400579153607E-4	0.4359503301120945
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6080	0.42202908530054645	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3258	0.22614650656400995	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2888	0.20046381551775955	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2346	0.16284214376892797	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.024155612119176016	0.0
2	0.0	0.0	0.0	0.08308697616854509	0.0
3	0.0	0.0	0.0	0.11112969828391035	0.0
4	0.0	0.0	0.0	0.14583603753560004	0.0
5	0.0	0.0	0.0	0.2430137874403311	0.0
6	0.0	0.0	0.0	0.33692914145540337	0.0
7	0.0	0.0	0.0	0.4014135197850428	0.0
8	0.0	0.0	0.0	0.5873700854955961	0.0
9	0.0	0.0	0.0	0.6863525650414151	0.0
10	0.0	0.0	0.0	0.8803610014583604	0.0
11	0.0	0.0	0.0	1.0733976603762583	0.0
12	0.0	0.0	0.0	1.236378629502193	0.0
13	0.0	0.0	0.0	1.3056524826485656	0.0
14	0.0	0.0	0.0	1.3323069511938634	0.0
15	0.0	0.0	0.0	1.3741627963314011	0.0
16	0.0	0.0	0.0	1.4655098812418483	0.0
17	0.0	0.0	0.0	1.5677547566773262	0.0
18	0.0	0.0	0.0	1.7266403777715615	0.0
19	0.0	0.0	0.0	1.793345961813309	0.0
20	0.0	0.0	0.0	1.871851701200631	0.0
21	0.0	0.0	0.0	1.9679188482493082	0.0
22	0.0	0.0	0.0	2.0693307715427456	0.0
23	0.0	0.0	0.0	2.1820569614322336	0.0
24	0.0	0.0	0.0	2.2724322688436334	0.0
25	6.941267850337935E-5	0.0	0.0	2.348230913769324	0.0
26	6.941267850337935E-5	0.0	0.0	2.4249319235155578	0.0
27	6.941267850337935E-5	0.0	0.0	2.498648188086147	0.0
28	1.388253570067587E-4	0.0	0.0	2.58374813193129	0.0
29	1.388253570067587E-4	0.0	0.0	2.668501012383916	0.0
30	1.388253570067587E-4	0.0	0.0	2.7813660276304106	0.0
31	1.388253570067587E-4	0.0	0.0	2.8813202846752772	0.0
32	1.388253570067587E-4	0.0	0.0	2.974819162619329	0.0
33	1.388253570067587E-4	0.0	0.0	3.0786605296603846	0.0
34	1.388253570067587E-4	0.0	0.0	3.1824324840229368	0.0
35	1.388253570067587E-4	0.0	0.0	3.321049602994185	0.0
36	2.0823803551013806E-4	0.0	0.0	3.4382182043078897	0.0
37	2.0823803551013806E-4	0.0	0.0	3.5514302829469013	0.0
38	2.0823803551013806E-4	0.0	0.0	3.6667247419410143	0.0
39	2.0823803551013806E-4	0.0	0.0	3.7887522307499553	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACTA	25	3.8912993E-5	45.0	26
CGTAGGA	20	7.0338824E-4	45.0	35
TATTCGA	20	7.0338824E-4	45.0	38
ACGTCCA	25	3.8912993E-5	45.0	12
TCATACG	25	3.8912993E-5	45.0	1
TTATTCG	20	7.0338824E-4	45.0	37
TATCGGA	25	3.8912993E-5	45.0	36
GCGTATT	20	7.0338824E-4	45.0	22
GCGATCG	30	2.165887E-6	44.999996	9
CGGTCTA	115	0.0	44.999996	31
TCGATGG	120	0.0	43.124996	2
TATAGCG	150	0.0	42.000004	1
AATACGG	70	0.0	41.785717	2
ACGGGAT	425	0.0	41.294117	5
TACGGGA	290	0.0	40.34483	4
ACGGGTA	95	0.0	40.263157	5
ACGGGAC	285	0.0	40.263157	5
TCGCCTA	45	1.9288564E-8	40.0	33
AGGGTAC	390	0.0	39.807693	6
CTCACGA	130	0.0	39.80769	24
>>END_MODULE