FastQCFastQC Report
Thu 26 May 2016
SRR1545249_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545249_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3529419
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228820.6483220042732245No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA99200.2810660904811812No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT68040.19277960480181014No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAG1300.041.5384641
ACGTTAG1150.041.086961
CGGTCTA2100.040.71428731
TTAACGG4500.040.02
ACATACG24950.039.13827517
CATACGA24700.039.0789518
TAGGGTA15150.038.613865
TAGGGCG18200.038.44785
TAAGTAG4100.038.4146351
ACGGGCT7450.038.3557055
CGTTTTT151850.038.3026051
TAATGCG659.094947E-1238.0769271
CGAGGGA13950.038.0645144
AGGGCGA39350.038.024146
TCTAGCG1250.037.81
GGACTAA46450.037.7825628
ATAGCGG5900.037.754242
GACCGAT14900.037.751689
GGACCGA15700.037.6910868
CATATGC21500.037.6744233
Produced by FastQC (version 0.11.5)