##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545249_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3529419
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14345222258961	31.0	31.0	33.0	30.0	34.0
2	31.415153032269618	31.0	31.0	34.0	30.0	34.0
3	31.516366574781856	31.0	31.0	34.0	30.0	34.0
4	35.28929350694831	37.0	35.0	37.0	33.0	37.0
5	35.14179075932894	37.0	35.0	37.0	32.0	37.0
6	35.13147603047414	37.0	35.0	37.0	32.0	37.0
7	35.788108184378224	37.0	35.0	37.0	35.0	37.0
8	35.86220366581582	37.0	35.0	37.0	35.0	37.0
9	37.476192540471956	39.0	37.0	39.0	35.0	39.0
10	36.68740860747902	39.0	35.0	39.0	32.0	39.0
11	36.37498154795449	38.0	35.0	39.0	32.0	39.0
12	35.63747602650748	37.0	35.0	39.0	31.0	39.0
13	35.29487261217781	37.0	35.0	39.0	30.0	39.0
14	36.11920120563753	38.0	35.0	40.0	30.0	41.0
15	36.49534101788424	38.0	35.0	40.0	31.0	41.0
16	36.37075620661644	38.0	35.0	40.0	31.0	41.0
17	36.42102255357043	38.0	35.0	40.0	31.0	41.0
18	36.463870682398436	38.0	35.0	40.0	31.0	41.0
19	36.377576592634654	38.0	35.0	40.0	31.0	41.0
20	36.24661311110979	37.0	35.0	40.0	31.0	41.0
21	36.13141256393758	37.0	34.0	40.0	30.0	41.0
22	36.01459220341932	37.0	34.0	40.0	30.0	41.0
23	35.929794960586996	37.0	34.0	40.0	30.0	41.0
24	35.7230498844144	37.0	34.0	40.0	30.0	41.0
25	35.67300057034883	37.0	34.0	40.0	30.0	41.0
26	35.5313415607498	36.0	34.0	40.0	29.0	41.0
27	35.49548353425875	37.0	34.0	40.0	29.0	41.0
28	35.4979981124372	36.0	34.0	40.0	29.0	41.0
29	35.453007138002036	36.0	34.0	40.0	29.0	41.0
30	35.33214758576412	36.0	34.0	40.0	29.0	41.0
31	35.208162306600606	36.0	34.0	40.0	28.0	41.0
32	35.06885807550761	36.0	34.0	40.0	27.0	41.0
33	34.883616255253344	36.0	34.0	40.0	27.0	41.0
34	34.703332758167846	36.0	34.0	40.0	26.0	41.0
35	34.53170592666952	36.0	33.0	40.0	25.0	41.0
36	34.320339126638125	36.0	33.0	40.0	24.0	41.0
37	34.131281097540416	35.0	33.0	40.0	23.0	41.0
38	34.16546887745547	35.0	33.0	40.0	24.0	41.0
39	34.14456005365189	35.0	33.0	40.0	23.0	41.0
40	34.01612106695181	35.0	33.0	40.0	23.0	41.0
41	33.967335700295145	35.0	33.0	40.0	23.0	41.0
42	33.87329132641945	35.0	33.0	40.0	23.0	41.0
43	33.73179919981164	35.0	33.0	40.0	23.0	41.0
44	33.67118214074328	35.0	33.0	39.0	23.0	41.0
45	33.60683755598301	35.0	33.0	39.0	23.0	41.0
46	33.527547451861054	35.0	32.0	39.0	23.0	41.0
47	33.50371095072588	35.0	32.0	39.0	23.0	41.0
48	33.45971164092447	35.0	32.0	39.0	23.0	41.0
49	33.4300413184153	35.0	32.0	39.0	23.0	41.0
50	33.23715914715708	35.0	32.0	39.0	23.0	41.0
51	33.07638226008304	35.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	3.0
12	5.0
13	14.0
14	45.0
15	143.0
16	432.0
17	1207.0
18	2496.0
19	4666.0
20	8081.0
21	12659.0
22	18870.0
23	26301.0
24	36260.0
25	49431.0
26	64145.0
27	75323.0
28	84860.0
29	97409.0
30	114339.0
31	139027.0
32	170160.0
33	216589.0
34	342907.0
35	471164.0
36	273318.0
37	323500.0
38	424203.0
39	571433.0
40	426.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.957033721414206	23.770711270041897	27.332090635880864	14.940164372663038
2	32.12041415315098	26.018899994588345	28.684664529771048	13.176021322489623
3	29.571411045274026	25.41744122757882	31.556695308774618	13.454452418372542
4	26.922703141791892	27.57184681104737	30.589085625707803	14.916364421452935
5	22.76946998925319	32.23638224874972	29.84522381729117	15.148923944705913
6	21.277354714756168	41.352698560301285	27.193030920953277	10.176915803989269
7	85.36640166554325	4.967616483052876	7.711127525521906	1.954854325881965
8	86.27232980839055	4.378652690428651	7.514437928735579	1.8345795724452096
9	81.63386098391831	5.929729510721169	9.245742712894105	3.1906667924664087
10	48.24527776384725	23.780627916379437	15.332155235748434	12.641939084024878
11	40.987964308006504	22.050088130652664	22.2608026986878	14.701144862653031
12	37.49738979701758	21.0474018528262	26.919076482559877	14.536131867596339
13	21.803418636325127	36.41709867828104	25.836660368179576	15.942822317214251
14	16.242248370057506	37.53255139160298	28.953745644821428	17.271454593518083
15	15.638069608623969	23.834347806253664	43.537335748461715	16.990246836660653
16	17.520674082618132	19.646633057735567	41.941209020521505	20.891483839124795
17	17.283241236022132	20.170090317981515	29.922290325971503	32.62437812002486
18	20.56621783925343	23.437398619999495	35.113683016949814	20.88270052379726
19	29.487969549662424	24.19038374304666	26.210007936150397	20.11163877114052
20	30.430192618105135	23.33828882317458	26.97718803009787	19.254330528622415
21	22.51058885329285	28.7446177402003	28.43091171663098	20.313881689875867
22	22.86036313625557	24.46405484868756	24.72812097401867	27.947461041038203
23	20.336916642654217	29.909087019704945	26.355272638357757	23.39872369928308
24	19.40356188936479	24.203133716909214	39.15885305768457	17.234451336041428
25	18.754219887182565	26.141186410567858	34.92413340552652	20.18046029672306
26	17.521722413802383	33.12477209421721	27.21685920543863	22.13664628654178
27	17.8340117736092	33.280803441019614	28.54050482529844	20.34467996007275
28	16.804918883249623	28.691322849454824	35.73942340084869	18.76433486644686
29	17.045270057196383	25.257613221892893	34.929771727301286	22.767344993609427
30	19.241750554411365	31.878192983037717	30.36074776046709	18.51930870208383
31	28.357103534604423	28.081647432622763	25.266084871192678	18.295164161580136
32	29.615610954664213	25.1158901790918	26.533403939855255	18.735094926388733
33	27.94066105497817	26.77126178558001	24.135842188190182	21.152234971251644
34	19.6190081143667	27.011527959701016	27.515661926226386	25.8538019997059
35	21.228564814775464	25.150145108869193	30.997679788089766	22.623610288265574
36	29.40175139307631	27.870111199605375	23.973719187209	18.754418220109315
37	20.306231705558336	34.558974154102984	27.261200781205062	17.873593359133615
38	20.740665814968413	34.064501834437905	24.90166228492565	20.293170065668033
39	20.64101768591374	33.19305528757	25.624075803977934	20.541851222538327
40	24.934330551289037	25.806542096588704	26.997644654828456	22.261482697293804
41	18.48227711133192	24.710186010785346	28.199088858534505	28.608448019348227
42	19.74528952215648	26.245254530561546	25.15159010590695	28.857865841375023
43	20.017373964383374	25.928516846540468	27.430605433925525	26.623503755150637
44	18.702993325530347	28.60686135593422	30.229366363132286	22.46077895540314
45	18.182511059185664	36.554911729097626	24.084218960684463	21.178358251032254
46	21.150308308534633	34.16701162429284	24.8660756912115	19.81660437596103
47	20.35201827836253	28.965503954050227	27.04105123251164	23.641426535075603
48	20.603192763454835	26.313707723565834	32.3259153985401	20.75718411443923
49	21.04978184794721	27.021727938791056	31.834248073124783	20.094242140136945
50	19.682077985073466	33.449188095831076	27.245589146542248	19.623144772553218
51	18.02749971029226	32.70815961493946	26.784153425818815	22.480187248949473
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	11217.0
1	10592.0
2	9967.0
3	24660.0
4	39353.0
5	28256.0
6	17159.0
7	16775.0
8	16391.0
9	17511.5
10	18632.0
11	19636.5
12	20641.0
13	20190.0
14	19739.0
15	19755.0
16	19771.0
17	18371.5
18	16972.0
19	16581.0
20	16190.0
21	16253.5
22	16317.0
23	17816.5
24	19316.0
25	21794.0
26	28980.5
27	33689.0
28	41277.5
29	48866.0
30	56775.5
31	64685.0
32	74204.5
33	83724.0
34	96949.5
35	110175.0
36	114180.5
37	118186.0
38	135538.0
39	152890.0
40	195980.5
41	239071.0
42	277396.5
43	315722.0
44	320371.5
45	325021.0
46	305335.5
47	285650.0
48	261203.5
49	236757.0
50	217762.0
51	198767.0
52	183778.5
53	168790.0
54	154366.0
55	139942.0
56	138428.5
57	136915.0
58	132956.0
59	128997.0
60	121849.5
61	114702.0
62	104765.0
63	94828.0
64	84302.5
65	73777.0
66	62695.0
67	51613.0
68	46647.0
69	41681.0
70	37721.0
71	33761.0
72	30100.0
73	26439.0
74	22444.5
75	14161.0
76	9872.0
77	7727.5
78	5583.0
79	4198.5
80	2814.0
81	2034.0
82	1254.0
83	888.0
84	522.0
85	362.0
86	202.0
87	146.0
88	90.0
89	60.5
90	31.0
91	19.5
92	8.0
93	6.5
94	5.0
95	3.5
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3529419.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.58068758964544
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.95637423123158	13.88107348570414
2	7.021083423936196	2.4687094843412076
3	2.5727400725985743	1.3569161839715178
4	1.4618758535712035	1.0280313070592637
5	1.0229246591901286	0.8991859430483093
6	0.7034996655941219	0.7420804704138179
7	0.5621332311347237	0.6917882104236269
8	0.4460717833223855	0.6273798932117497
9	0.36748572132522916	0.5814586494246923
>10	4.25102900623267	17.87180602834377
>50	1.5293492030750495	19.032901067590853
>100	1.0800939978150126	36.09034191044548
>500	0.02017129781416276	2.2245263134992515
>1k	0.00466773833716163	1.3430333629693976
>5k	3.334098812258307E-4	0.49014489824986807
>10k+	1.6670494061291535E-4	0.6706227913031261
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22882	0.6483220042732245	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9920	0.2810660904811812	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	6804	0.19277960480181014	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02073995748308716	0.0
2	0.0	0.0	0.0	0.06372153603751779	0.0
3	0.0	0.0	0.0	0.09919479665066687	0.0
4	0.0	0.0	0.0	0.1399663797355882	0.0
5	0.0	0.0	0.0	0.247944491713792	0.0
6	0.0	0.0	0.0	0.3930675275449019	0.0
7	0.0	0.0	0.0	0.49084566043306277	0.0
8	0.0	0.0	0.0	0.7401501493588605	0.0
9	0.0	0.0	0.0	0.8491765925213187	0.0
10	0.0	0.0	0.0	1.0233695687590507	0.0
11	0.0	0.0	0.0	1.1701359345546676	0.0
12	2.8333275250119072E-5	0.0	0.0	1.2973806737029523	0.0
13	5.6666550500238143E-5	0.0	0.0	1.3541605573041908	0.0
14	5.6666550500238143E-5	0.0	0.0	1.3797455048550484	0.0
15	5.6666550500238143E-5	0.0	0.0	1.41346210240269	0.0
16	5.6666550500238143E-5	0.0	0.0	1.483615291921985	0.0
17	8.499982575035722E-5	0.0	0.0	1.5638834607055723	0.0
18	8.499982575035722E-5	0.0	0.0	1.6931398623966154	0.0
19	1.1333310100047629E-4	0.0	0.0	1.7477947503540952	0.0
20	1.1333310100047629E-4	0.0	0.0	1.81086462106086	0.0
21	1.1333310100047629E-4	0.0	0.0	1.8924077872307028	0.0
22	1.1333310100047629E-4	0.0	0.0	1.979305942422818	0.0
23	1.1333310100047629E-4	0.0	0.0	2.08442239360076	0.0
24	1.4166637625059536E-4	0.0	0.0	2.1670705575053573	0.0
25	1.6999965150071443E-4	0.0	0.0	2.2390087433654093	0.0
26	1.6999965150071443E-4	0.0	0.0	2.3093885990867054	0.0
27	1.6999965150071443E-4	0.0	0.0	2.379740121532751	0.0
28	1.6999965150071443E-4	0.0	0.0	2.458761626205333	0.0
29	1.6999965150071443E-4	0.0	0.0	2.551128103520721	0.0
30	1.6999965150071443E-4	0.0	0.0	2.66987286009397	0.0
31	1.6999965150071443E-4	0.0	0.0	2.768614324340635	0.0
32	1.6999965150071443E-4	0.0	0.0	2.865514125696042	0.0
33	1.983329267508335E-4	0.0	0.0	2.9647939221724595	0.0
34	1.983329267508335E-4	0.0	0.0	3.070108706277152	0.0
35	1.983329267508335E-4	0.0	0.0	3.2037000990814635	0.0
36	1.983329267508335E-4	0.0	0.0	3.3180815312661944	0.0
37	1.983329267508335E-4	0.0	0.0	3.427957972686156	0.0
38	2.549994772510716E-4	0.0	0.0	3.5531910492916823	0.0
39	3.1166602775130976E-4	0.0	0.0	3.7038957403470656	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATAG	130	0.0	41.538464	1
ACGTTAG	115	0.0	41.08696	1
CGGTCTA	210	0.0	40.714287	31
TTAACGG	450	0.0	40.0	2
ACATACG	2495	0.0	39.138275	17
CATACGA	2470	0.0	39.07895	18
TAGGGTA	1515	0.0	38.61386	5
TAGGGCG	1820	0.0	38.4478	5
TAAGTAG	410	0.0	38.414635	1
ACGGGCT	745	0.0	38.355705	5
CGTTTTT	15185	0.0	38.302605	1
TAATGCG	65	9.094947E-12	38.076927	1
CGAGGGA	1395	0.0	38.064514	4
AGGGCGA	3935	0.0	38.02414	6
TCTAGCG	125	0.0	37.8	1
GGACTAA	4645	0.0	37.782562	8
ATAGCGG	590	0.0	37.75424	2
GACCGAT	1490	0.0	37.75168	9
GGACCGA	1570	0.0	37.691086	8
CATATGC	2150	0.0	37.67442	33
>>END_MODULE