FastQCFastQC Report
Thu 26 May 2016
SRR1545248_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545248_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3271258
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140000.42796991249238064No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA71050.21719473058988314No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC69040.21105030541767114No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG51840.1584711447400358No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT39360.12032068396928644No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGCGT502.1827873E-1145.00000438
TCCGATT351.2128476E-745.033
GACCGAC351.2128476E-745.09
CATACGT406.8212103E-945.027
GCGTATT351.2128476E-745.09
TCTATCG406.8212103E-945.01
CGTAAGG4600.043.0434762
ACGTAAG800.042.18751
ACGGGTA3600.041.8755
ACTACGG1350.041.6666682
CGGTCTA2000.041.62500431
TATACGG2200.040.9090922
ACGTATA501.0822987E-940.50000413
CTACGAA5100.040.1470611
CGTACAT5150.039.75728235
GCTACGA5150.039.75728210
GGTATGC14550.039.5876278
TGCGTAG2050.039.5121961
GCGTAAG2450.039.4897961
CGCATCG800.039.37521