##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545248_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3271258
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.265791019846187	31.0	31.0	33.0	30.0	34.0
2	31.52771685999698	31.0	31.0	34.0	30.0	34.0
3	31.62213313654869	31.0	31.0	34.0	30.0	34.0
4	35.3741902962102	37.0	35.0	37.0	33.0	37.0
5	35.235319867769526	37.0	35.0	37.0	32.0	37.0
6	35.22676933461072	37.0	35.0	37.0	32.0	37.0
7	35.86114057649993	37.0	35.0	37.0	35.0	37.0
8	35.94558545978336	37.0	35.0	37.0	35.0	37.0
9	37.56515352809225	39.0	37.0	39.0	35.0	39.0
10	36.81640274169754	39.0	37.0	39.0	32.0	39.0
11	36.49023678352487	39.0	35.0	39.0	32.0	39.0
12	35.564557121449916	37.0	35.0	39.0	31.0	39.0
13	35.134542735547	37.0	35.0	39.0	30.0	39.0
14	35.920414715072916	38.0	35.0	40.0	29.0	41.0
15	36.35061068249585	38.0	35.0	40.0	31.0	41.0
16	36.2715704478216	38.0	35.0	40.0	31.0	41.0
17	36.35509030470846	38.0	35.0	40.0	31.0	41.0
18	36.43299152802989	37.0	35.0	40.0	31.0	41.0
19	36.368588475748474	37.0	35.0	40.0	31.0	41.0
20	36.249313872522436	37.0	35.0	40.0	31.0	41.0
21	36.11748752314859	37.0	34.0	40.0	31.0	41.0
22	36.00363040762912	37.0	34.0	40.0	30.0	41.0
23	35.94969366525049	37.0	34.0	40.0	30.0	41.0
24	35.75305677509998	36.0	34.0	40.0	30.0	41.0
25	35.72212891798812	36.0	34.0	40.0	30.0	41.0
26	35.58821193559175	36.0	34.0	40.0	30.0	41.0
27	35.55395447256071	36.0	34.0	40.0	30.0	41.0
28	35.612715658624296	36.0	34.0	40.0	30.0	41.0
29	35.6219903780136	36.0	34.0	40.0	30.0	41.0
30	35.53418348537474	36.0	34.0	40.0	30.0	41.0
31	35.40331364875531	36.0	34.0	40.0	29.0	41.0
32	35.26362640916736	36.0	34.0	40.0	29.0	41.0
33	35.09460886301233	36.0	34.0	40.0	28.0	41.0
34	34.94082398881409	36.0	34.0	40.0	27.0	41.0
35	34.78603521947826	35.0	34.0	40.0	27.0	41.0
36	34.573008610143255	35.0	33.0	40.0	25.0	41.0
37	34.388061718152464	35.0	33.0	40.0	25.0	41.0
38	34.45204046883492	35.0	33.0	40.0	26.0	41.0
39	34.46494406738937	35.0	33.0	40.0	26.0	41.0
40	34.28694710108466	35.0	33.0	40.0	24.0	41.0
41	34.31827174744395	35.0	33.0	40.0	25.0	41.0
42	34.24303861083412	35.0	33.0	40.0	25.0	41.0
43	34.13403436842952	35.0	33.0	40.0	24.0	41.0
44	34.09124685365691	35.0	33.0	40.0	24.0	41.0
45	34.04384398907087	35.0	33.0	39.0	24.0	41.0
46	33.985228007084736	35.0	33.0	39.0	24.0	41.0
47	33.99625893157923	35.0	33.0	39.0	24.0	41.0
48	33.95093477799672	35.0	33.0	39.0	24.0	41.0
49	33.93481131723637	35.0	33.0	39.0	24.0	41.0
50	33.73951397291195	35.0	33.0	39.0	24.0	41.0
51	33.56237264073943	35.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	7.0
13	6.0
14	39.0
15	89.0
16	270.0
17	650.0
18	1635.0
19	3211.0
20	5615.0
21	9211.0
22	13658.0
23	19793.0
24	28407.0
25	39044.0
26	51543.0
27	62192.0
28	71062.0
29	82672.0
30	100836.0
31	124588.0
32	158219.0
33	207232.0
34	343885.0
35	501185.0
36	239020.0
37	279462.0
38	378953.0
39	548334.0
40	439.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.12594115169149	24.934505318748933	28.371317701018995	13.568235828540578
2	31.13930481790186	26.50347358722546	29.714531840655795	12.64268975421688
3	29.818100559478957	25.379991428374037	30.768805150801313	14.033102861345698
4	26.112400795045822	28.8408312643026	30.160690474429103	14.886077466222474
5	23.0293972532891	33.63079280203518	28.92352116525202	14.416288779423695
6	21.57185400845791	41.61111719100114	26.749647994746972	10.067380805793979
7	86.73042603182017	5.309486442218865	6.187925256888939	1.7721622690720207
8	88.41543528514106	3.907701563129536	6.098846376531597	1.5780167751977985
9	83.48827882117521	5.874926404459691	7.526462296767787	3.1103324775973036
10	51.79716793967336	23.722953065762468	13.465217356747772	11.0146616378164
11	45.84713281557126	20.588623703786126	19.68010471812373	13.884138762518884
12	41.14863456199419	21.321185916855228	24.524938112493725	13.005241408656854
13	20.678161123335425	41.1370793743569	24.019566784399153	14.165192717908523
14	15.048339201616015	42.88221840038297	26.849028722283595	15.220413675717415
15	14.394554021724975	23.406988993225237	47.63827860719026	14.560178377859526
16	16.198752895674996	18.917584611180164	45.15690905455944	19.7267534385854
17	16.03652172956092	19.09500870918772	29.210719545813873	35.657750015437486
18	19.844536872359196	23.347715160345043	37.05146460474838	19.756283362547375
19	30.82401326951283	23.39470014288081	26.544650406663123	19.236636180943233
20	31.970116695167427	22.009850644614396	26.137009065014134	19.883023595204047
21	21.769148138116893	30.209754167968413	27.913573310328932	20.107524383585766
22	21.764960146830365	24.822438340234857	23.42967139858733	29.982930114347443
23	19.285913859438786	31.265769927043358	24.877004504077636	24.57131170944022
24	17.713124431029286	23.896372588160272	41.557376397703884	16.833126583106562
25	16.865927419971154	25.79863771062998	37.74566237209049	19.589772497308374
26	15.700259655459764	35.634303378088795	27.896607360226554	20.768829606224884
27	16.174175194986148	37.18147575030768	27.842438597016805	18.801910457689367
28	14.608141577338138	30.18869193441789	37.5174321316142	17.685734356629773
29	15.23704336374569	25.933111970990975	36.53634167650488	22.293502988758455
30	17.599223295747386	33.98634409147795	30.416127373628125	17.998305239146532
31	30.187713717475052	28.36129097735489	24.330884326457895	17.120110978712166
32	31.416659890476385	25.46540199519573	26.33164366735977	16.786294446968107
33	29.691666019616918	26.800729260730886	23.59945317672895	19.908151542923243
34	19.21104969403208	26.519033350472508	26.72834120696075	27.54157574853466
35	20.182969365302277	25.64985702747995	32.43415835742702	21.733015249790753
36	32.238606676697465	26.698902990837166	23.685903099052414	17.37658723341296
37	19.519035184629278	37.48371421636569	26.17577091137416	16.821479687630873
38	19.58521767466828	36.762493205977634	24.321346711265207	19.330942408088873
39	20.74036960704414	34.87698004865407	25.219777834704573	19.162872509597225
40	26.035885888548076	25.292624427666667	26.260967493239605	22.410522190545656
41	16.848258376441112	25.131432617054354	27.296532404353307	30.72377660215122
42	19.104485185821478	25.075704820591955	25.9851103153588	29.834699678227768
43	19.205975193641102	25.235887844981963	27.62016936603594	27.937967595340996
44	17.440477027492175	29.43332503886884	31.381505219093082	21.744692714545902
45	16.643199649798333	40.05593566756275	22.424186658465945	20.87667802417296
46	19.535084056347742	36.29527233865382	25.24673993919159	18.922903665806853
47	20.847820624359194	28.08683998632942	26.15088751789067	24.91445187142072
48	20.732482732942493	25.674526436007188	33.73815211151184	19.854838719538478
49	20.315701176733842	26.53972875266946	34.018533542753275	19.126036527843418
50	18.825418233596984	36.342257321189585	26.367440293611814	18.464884151601616
51	17.63691521732618	34.760541663176674	26.286767965107	21.315775154390145
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	8312.0
1	7927.0
2	7542.0
3	16402.0
4	25262.0
5	18396.0
6	11530.0
7	11678.5
8	11827.0
9	12470.5
10	13114.0
11	13893.5
12	14673.0
13	14866.5
14	15060.0
15	14243.0
16	13426.0
17	12666.0
18	11906.0
19	11551.5
20	11197.0
21	11680.5
22	12164.0
23	13707.0
24	15250.0
25	17855.5
26	26255.5
27	32050.0
28	40483.0
29	48916.0
30	54734.5
31	60553.0
32	71267.0
33	81981.0
34	90406.5
35	98832.0
36	105770.0
37	112708.0
38	134075.5
39	155443.0
40	206031.0
41	256619.0
42	305405.0
43	354191.0
44	358539.0
45	362887.0
46	336523.5
47	310160.0
48	273622.5
49	237085.0
50	211390.0
51	185695.0
52	166940.5
53	148186.0
54	133862.5
55	119539.0
56	110276.5
57	101014.0
58	97990.0
59	94966.0
60	90795.0
61	86624.0
62	76935.0
63	67246.0
64	59484.5
65	51723.0
66	42979.5
67	34236.0
68	30092.5
69	25949.0
70	23465.0
71	20981.0
72	17112.0
73	13243.0
74	11264.0
75	7259.5
76	5234.0
77	3959.0
78	2684.0
79	1774.5
80	865.0
81	678.5
82	492.0
83	278.5
84	65.0
85	61.0
86	57.0
87	33.0
88	9.0
89	7.0
90	5.0
91	6.5
92	8.0
93	4.0
94	0.0
95	0.5
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3271258.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.621295524278974
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.88236108988254	12.3224467423794
2	8.01899066079074	2.5053404583729058
3	2.9243170233698974	1.3704486128622297
4	1.5324456413608676	0.9575514495436538
5	0.9469331967539839	0.7396161654122096
6	0.6446299847120867	0.6041973296999366
7	0.44656511887565087	0.48831479849539206
8	0.3374689103729235	0.4217361263353482
9	0.2754351670756396	0.38723887284009484
>10	3.1994657888680043	12.272865362059566
>50	1.3668913704122068	15.527588200047063
>100	1.3776816307034785	45.8359135979641
>500	0.03951866355103861	4.0529634397789245
>1k	0.006485114018631978	1.4629519189178808
>5k	6.079794392467479E-4	0.6076136889439936
>10k+	2.0265981308224931E-4	0.4432132363474136
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14000	0.42796991249238064	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7105	0.21719473058988314	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6904	0.21105030541767114	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	5184	0.1584711447400358	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	3936	0.12032068396928644	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.0569279463741473E-5	0.0	0.0	0.027909752150395963	0.0
2	3.0569279463741473E-5	0.0	0.0	0.09458135066081612	0.0
3	3.0569279463741473E-5	0.0	0.0	0.1312644860173059	0.0
4	3.0569279463741473E-5	0.0	0.0	0.1822846134422904	0.0
5	3.0569279463741473E-5	0.0	0.0	0.32238362122461756	0.0
6	3.0569279463741473E-5	0.0	0.0	0.45404550787495207	0.0
7	3.0569279463741473E-5	0.0	0.0	0.5483517350205945	0.0
8	3.0569279463741473E-5	0.0	0.0	0.777804746675438	0.0
9	3.0569279463741473E-5	0.0	0.0	0.8803952485557544	0.0
10	3.0569279463741473E-5	0.0	0.0	1.060417735317728	0.0
11	3.0569279463741473E-5	0.0	0.0	1.2316668388736076	0.0
12	3.0569279463741473E-5	0.0	0.0	1.390321399290426	0.0
13	3.0569279463741473E-5	0.0	0.0	1.450848572628634	0.0
14	3.0569279463741473E-5	0.0	0.0	1.4727667460041367	0.0
15	3.0569279463741473E-5	0.0	0.0	1.513760149765014	0.0
16	3.0569279463741473E-5	0.0	0.0	1.596511189273362	0.0
17	3.0569279463741473E-5	0.0	0.0	1.7081807671544098	0.0
18	3.0569279463741473E-5	0.0	0.0	1.8912296125832937	0.0
19	3.0569279463741473E-5	0.0	0.0	1.9609887083195516	0.0
20	3.0569279463741473E-5	0.0	0.0	2.038634678157455	0.0
21	3.0569279463741473E-5	0.0	0.0	2.1402775323743954	0.0
22	3.0569279463741473E-5	0.0	0.0	2.248523350955504	0.0
23	3.0569279463741473E-5	0.0	0.0	2.3794515748987086	0.0
24	3.0569279463741473E-5	0.0	0.0	2.482286631014735	0.0
25	3.0569279463741473E-5	0.0	0.0	2.561430495546362	0.0
26	3.0569279463741473E-5	0.0	0.0	2.644150965775246	0.0
27	6.113855892748295E-5	0.0	0.0	2.7245176014854224	0.0
28	6.113855892748295E-5	0.0	0.0	2.8135353432838377	0.0
29	6.113855892748295E-5	0.0	0.0	2.912212977392795	0.0
30	9.170783839122441E-5	0.0	0.0	3.0452810508984616	3.0569279463741473E-5
31	9.170783839122441E-5	0.0	0.0	3.164531810086517	3.0569279463741473E-5
32	9.170783839122441E-5	0.0	0.0	3.2776687133818245	3.0569279463741473E-5
33	9.170783839122441E-5	0.0	0.0	3.3886963363941334	3.0569279463741473E-5
34	9.170783839122441E-5	0.0	0.0	3.50403422781083	3.0569279463741473E-5
35	9.170783839122441E-5	0.0	0.0	3.6664488096016883	3.0569279463741473E-5
36	1.222771178549659E-4	0.0	0.0	3.7928527801842593	3.0569279463741473E-5
37	1.5284639731870736E-4	0.0	0.0	3.9161692535409927	3.0569279463741473E-5
38	1.5284639731870736E-4	0.0	0.0	4.045385597834228	3.0569279463741473E-5
39	1.5284639731870736E-4	0.0	0.0	4.184047849481759	3.0569279463741473E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATGCGT	50	2.1827873E-11	45.000004	38
TCCGATT	35	1.2128476E-7	45.0	33
GACCGAC	35	1.2128476E-7	45.0	9
CATACGT	40	6.8212103E-9	45.0	27
GCGTATT	35	1.2128476E-7	45.0	9
TCTATCG	40	6.8212103E-9	45.0	1
CGTAAGG	460	0.0	43.043476	2
ACGTAAG	80	0.0	42.1875	1
ACGGGTA	360	0.0	41.875	5
ACTACGG	135	0.0	41.666668	2
CGGTCTA	200	0.0	41.625004	31
TATACGG	220	0.0	40.909092	2
ACGTATA	50	1.0822987E-9	40.500004	13
CTACGAA	510	0.0	40.14706	11
CGTACAT	515	0.0	39.757282	35
GCTACGA	515	0.0	39.757282	10
GGTATGC	1455	0.0	39.587627	8
TGCGTAG	205	0.0	39.512196	1
GCGTAAG	245	0.0	39.489796	1
CGCATCG	80	0.0	39.375	21
>>END_MODULE