FastQCFastQC Report
Thu 26 May 2016
SRR1545247_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545247_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1257924
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50450.40105761556342034No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45530.361945554739396No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC42300.33626832781630683No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT14060.11177145837109395No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13900.11049952143372731No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACG406.8157533E-945.0000041
TCGCAAT207.03354E-445.00000411
GATCGAC253.8910155E-545.0000049
ACGCATT207.03354E-445.00000443
TAGACTC207.03354E-445.00000434
TAAGTCG406.8157533E-945.0000041
ATAGACG406.8157533E-945.0000041
TCGATGC207.03354E-445.00000420
ATCGTAT406.8157533E-945.00000413
ATCCGAT207.03354E-445.00000432
ACTACGG207.03354E-445.0000042
TACTCTA207.03354E-445.00000428
GCGATCG253.8910155E-545.00000433
ACATGTC253.8910155E-545.00000419
ACGTAAG207.03354E-445.0000041
GTCCACA207.03354E-445.00000421
CCAGTAC406.8157533E-945.00000433
CGATCGC253.8910155E-545.00000434
GCATGCG253.8910155E-545.0000041
ATGCACG650.045.0000041