##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545247_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1257924
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21322273841663	31.0	31.0	33.0	30.0	34.0
2	31.463229098101316	31.0	31.0	34.0	30.0	34.0
3	31.546231727830936	31.0	31.0	34.0	30.0	34.0
4	35.29670472937952	37.0	35.0	37.0	33.0	37.0
5	35.126585548888485	37.0	35.0	37.0	32.0	37.0
6	35.115249410934204	37.0	35.0	37.0	32.0	37.0
7	35.810805740251396	37.0	35.0	37.0	35.0	37.0
8	35.92703613254855	37.0	35.0	37.0	35.0	37.0
9	37.62740435829192	39.0	37.0	39.0	35.0	39.0
10	36.836534639612566	39.0	37.0	39.0	32.0	39.0
11	36.36566358539944	38.0	35.0	39.0	32.0	39.0
12	34.95613884463608	35.0	34.0	39.0	30.0	39.0
13	34.27234634206836	35.0	33.0	39.0	25.0	39.0
14	34.89459856080335	35.0	33.0	40.0	25.0	41.0
15	35.53377231056884	35.0	33.0	40.0	30.0	41.0
16	35.63050390961616	35.0	34.0	40.0	31.0	41.0
17	35.73434166134043	35.0	34.0	40.0	31.0	41.0
18	35.84169790861769	36.0	34.0	40.0	31.0	41.0
19	35.73931493476553	36.0	34.0	40.0	30.0	41.0
20	35.51935331546262	35.0	34.0	40.0	30.0	41.0
21	35.37189130662902	35.0	34.0	40.0	30.0	41.0
22	35.24547587930591	35.0	34.0	40.0	30.0	41.0
23	35.211434872059044	35.0	34.0	40.0	29.0	41.0
24	35.04970888543346	35.0	34.0	40.0	29.0	41.0
25	35.059703129918816	35.0	34.0	39.0	29.0	41.0
26	34.90179454402651	35.0	34.0	39.0	29.0	41.0
27	34.7996111052814	35.0	34.0	39.0	29.0	41.0
28	34.93297528308546	35.0	34.0	39.0	29.0	41.0
29	34.99947930081627	35.0	34.0	39.0	29.0	41.0
30	34.945977658427694	36.0	34.0	39.0	29.0	41.0
31	34.75582308629138	35.0	34.0	39.0	29.0	41.0
32	34.554672619331534	35.0	34.0	39.0	28.0	41.0
33	34.327346485161264	35.0	33.0	39.0	27.0	41.0
34	34.17879379040387	35.0	33.0	39.0	26.0	41.0
35	33.99887274588926	35.0	33.0	39.0	25.0	41.0
36	33.704967072732536	35.0	33.0	39.0	23.0	41.0
37	33.501314864809004	35.0	33.0	39.0	23.0	41.0
38	33.660524006219774	35.0	33.0	39.0	23.0	41.0
39	33.67535161106712	35.0	33.0	39.0	23.0	41.0
40	33.52065148609932	35.0	33.0	39.0	23.0	41.0
41	33.626587933770246	35.0	33.0	39.0	23.0	41.0
42	33.58426025737644	35.0	33.0	39.0	23.0	41.0
43	33.399234770940055	35.0	33.0	38.0	23.0	41.0
44	33.38897659954019	35.0	33.0	38.0	23.0	41.0
45	33.3206290682108	35.0	32.0	38.0	23.0	40.0
46	33.24989824504501	35.0	32.0	38.0	23.0	40.0
47	33.28018544840547	35.0	32.0	38.0	23.0	40.0
48	33.333422368918946	35.0	33.0	38.0	23.0	40.0
49	33.38464565426846	35.0	33.0	38.0	24.0	40.0
50	33.12961673360235	35.0	32.0	38.0	24.0	40.0
51	32.932619935703585	35.0	32.0	38.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	3.0
13	5.0
14	12.0
15	36.0
16	121.0
17	366.0
18	764.0
19	1589.0
20	2778.0
21	4369.0
22	6967.0
23	10089.0
24	14178.0
25	18994.0
26	23794.0
27	27572.0
28	31453.0
29	35875.0
30	42935.0
31	52709.0
32	66509.0
33	88549.0
34	156673.0
35	231010.0
36	75698.0
37	84658.0
38	114610.0
39	165444.0
40	163.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.447289343394353	23.902159430935413	30.252543078914147	14.398008146756084
2	28.242008261230406	27.57328741640989	30.880800429914686	13.303903892445012
3	25.58071870796646	26.9796903469526	33.50782718192832	13.931763763152624
4	24.062661973219367	28.638137121161535	32.688779290322785	14.610421615296312
5	22.937236271825643	33.00223224932508	30.329097783331903	13.731433695517376
6	20.807457366263783	40.64736820348447	27.78872173517637	10.756452695075378
7	86.72900747580935	5.122328534951237	6.483857530343645	1.6648064588957678
8	89.26978100425781	3.895068382509595	5.4491368317958795	1.3860137814367164
9	86.29257411417542	4.388738906325025	6.498325813006191	2.8203611664933654
10	62.21154855142282	18.665992540089864	11.076026850588748	8.04643205789857
11	56.47415901119622	16.430563372667983	16.93432989592376	10.160947720212032
12	49.21378398059024	20.38803616116713	20.44368340217692	9.954496456065707
13	20.34264391171486	50.560526709085764	19.01339031610813	10.083439063091252
14	11.758262025368783	51.53260451346823	24.917960067539852	11.791173393623144
15	9.286332083655292	21.694474387959843	56.760344822103725	12.258848706281142
16	11.128573745313709	15.506342195553943	54.00946321081401	19.355620848318342
17	11.399416816914218	16.60823706360639	29.18125419341709	42.8110919260623
18	18.39316206702472	21.15429866987195	39.73992069473196	20.71261856837138
19	31.524003039929283	22.041236195509427	25.360117145391932	21.07464361916936
20	34.709966579856975	20.094218728635436	25.568635307061477	19.62717938444612
21	19.218569643317085	32.013539768698266	28.351315341785355	20.416575246199294
22	20.74417850362979	25.303197967444774	22.26366616743142	31.68895736149402
23	16.257341461010363	32.88688346831764	24.07546083865162	26.780314232020373
24	15.02698096228389	22.609235534102222	47.52369777506431	14.840085728549578
25	12.84147531965365	24.974004788842567	43.62624451079715	18.558275380706625
26	12.877407538134259	39.28528273568197	27.809470206467164	20.02783951971661
27	13.318451671166143	42.68676009043472	27.37828358469987	16.61650465369927
28	10.442522759721573	32.02840553165374	42.252314130265425	15.276757578359264
29	11.394647053399092	24.2134659963559	41.9237569201319	22.468130030113105
30	15.107033493279404	36.91693615830527	31.188768160874584	16.78726218754074
31	31.517563859183863	28.53018147360254	24.297811314515027	15.654443352698575
32	35.46708704182447	24.11552685217867	25.99075937815003	14.426626727846834
33	33.350504481987784	25.78661349970269	22.76059602964885	18.102285988660682
34	19.434639930552244	25.218137184758383	26.680705670612852	28.666517214076524
35	19.23494583138568	23.661524861597364	33.957377393228846	23.146151913788117
36	35.33329517522521	23.90533927327883	24.5023546732553	16.259010878240655
37	19.092488894400617	37.382783061615804	28.23143528543855	15.293292758545032
38	17.807832587660304	38.257398698172544	23.347117949892045	20.587650764275107
39	18.835637129111138	37.527227400065506	25.910547855037347	17.72658761578601
40	26.101258899583758	24.778682972898203	24.17236653406724	24.947691593450795
41	14.820529698137566	22.58133241753874	26.424330881674884	36.173807002648815
42	18.560342278229847	22.285130103249482	24.80944794757076	34.345079670949914
43	18.422496112642737	24.029273628613492	27.64483386913677	29.903396389607
44	15.791415061641246	29.732797847882704	32.33502182961769	22.140765260858366
45	13.403671445969708	44.86479310355793	22.132656662882656	19.59887878758971
46	20.02688556701359	38.746060970297094	24.317764825219964	16.909288637469356
47	18.94176436732267	28.458476028758493	25.873105211443615	26.72665439247522
48	20.222922847485222	23.441797755667274	36.05893519799289	20.27634419885462
49	20.65172458749495	23.309595810239728	37.145010350386826	18.893669251878492
50	18.610186306962902	38.75822386726066	25.455273927518675	17.176315898257766
51	15.50952203789736	37.422690083025685	24.505693507715886	22.56209437136107
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5046.0
1	4078.0
2	3110.0
3	5743.0
4	8376.0
5	5962.0
6	3548.0
7	3824.5
8	4101.0
9	4823.5
10	5546.0
11	6174.0
12	6802.0
13	6656.0
14	6510.0
15	6019.5
16	5529.0
17	5196.5
18	4864.0
19	4670.0
20	4476.0
21	4323.0
22	4170.0
23	4992.0
24	5814.0
25	5609.0
26	6676.5
27	7949.0
28	9986.5
29	12024.0
30	13826.5
31	15629.0
32	18830.5
33	22032.0
34	30106.0
35	38180.0
36	37389.5
37	36599.0
38	47561.5
39	58524.0
40	85214.5
41	111905.0
42	138269.0
43	164633.0
44	163125.5
45	161618.0
46	147692.0
47	133766.0
48	118662.0
49	103558.0
50	95079.0
51	86600.0
52	76936.0
53	67272.0
54	55325.5
55	43379.0
56	37680.5
57	31982.0
58	28596.0
59	25210.0
60	22835.5
61	20461.0
62	17075.0
63	13689.0
64	12062.0
65	10435.0
66	8785.0
67	7135.0
68	5993.5
69	4852.0
70	4157.5
71	3463.0
72	2583.0
73	1703.0
74	1321.0
75	781.0
76	623.0
77	388.5
78	154.0
79	157.0
80	160.0
81	101.5
82	43.0
83	66.5
84	90.0
85	51.5
86	13.0
87	10.0
88	7.0
89	4.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1257924.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.974637902140856
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.2697451309359	14.248449653235662
2	8.104265163016708	2.913424635363197
3	2.8580767882105054	1.541186860937927
4	1.3561388637727096	0.9750442008534073
5	0.8188837016098407	0.7359569010200822
6	0.5380309406115803	0.5802546802584845
7	0.3785024684758756	0.47624113711442434
8	0.3076881554255046	0.4424466544440866
9	0.25700043094782277	0.41575407182831414
>10	3.4095295788446722	15.263553087469116
>50	1.38964528479635	17.429962948092903
>100	1.2987979090740878	42.310274775800835
>500	0.010956467422769105	1.2155210471628843
>1k	0.0022825973797435633	1.037948135122077
>5k	4.565194759487127E-4	0.4139812112966146
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5045	0.40105761556342034	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4553	0.361945554739396	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4230	0.33626832781630683	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1406	0.11177145837109395	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1390	0.11049952143372731	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.033149856430118196	0.0
2	0.0	0.0	0.0	0.12838613461544576	0.0
3	0.0	0.0	0.0	0.1747323367707429	0.0
4	0.0	0.0	0.0	0.23785220728756268	0.0
5	0.0	0.0	0.0	0.43603588134100313	0.0
6	0.0	0.0	0.0	0.5741205351038695	0.0
7	0.0	0.0	0.0	0.6884358673496968	0.0
8	0.0	0.0	0.0	0.9703288910935796	0.0
9	0.0	0.0	0.0	1.1045182379857608	0.0
10	0.0	0.0	0.0	1.415029842820393	0.0
11	0.0	0.0	0.0	1.6710866475240158	0.0
12	0.0	0.0	0.0	1.9371599556094008	0.0
13	0.0	0.0	0.0	2.0279444545139453	0.0
14	0.0	0.0	0.0	2.0595043897723553	0.0
15	0.0	0.0	0.0	2.114436166254877	0.0
16	0.0	0.0	0.0	2.2408348994056873	0.0
17	0.0	0.0	0.0	2.3797940098129935	0.0
18	0.0	0.0	0.0	2.628696169243929	0.0
19	0.0	0.0	0.0	2.738321234033217	0.0
20	0.0	0.0	0.0	2.8433355274245504	0.0
21	0.0	0.0	0.0	2.969018796048092	0.0
22	7.949605858541534E-5	0.0	0.0	3.0931121434999254	0.0
23	7.949605858541534E-5	0.0	0.0	3.2411338045859686	0.0
24	1.5899211717083068E-4	0.0	0.0	3.356880065886333	0.0
25	1.5899211717083068E-4	0.0	0.0	3.4414638722212154	0.0
26	1.5899211717083068E-4	0.0	0.0	3.532963835653028	0.0
27	1.5899211717083068E-4	0.0	0.0	3.6254177517878663	0.0
28	1.5899211717083068E-4	0.0	0.0	3.7154072901065565	0.0
29	1.5899211717083068E-4	0.0	0.0	3.8092126392373467	0.0
30	1.5899211717083068E-4	0.0	0.0	3.969556189404129	0.0
31	1.5899211717083068E-4	0.0	0.0	4.093411048680206	0.0
32	1.5899211717083068E-4	0.0	0.0	4.1971534051341735	0.0
33	1.5899211717083068E-4	0.0	0.0	4.305109052693168	0.0
34	1.5899211717083068E-4	0.0	0.0	4.4210143061107035	0.0
35	1.5899211717083068E-4	0.0	0.0	4.593520753241054	0.0
36	1.5899211717083068E-4	0.0	0.0	4.718806541571669	0.0
37	1.5899211717083068E-4	0.0	0.0	4.847987636772968	0.0
38	3.1798423434166136E-4	0.0	0.0	4.969934590642996	0.0
39	3.9748029292707666E-4	0.0	0.0	5.106349827175569	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGTACG	40	6.8157533E-9	45.000004	1
TCGCAAT	20	7.03354E-4	45.000004	11
GATCGAC	25	3.8910155E-5	45.000004	9
ACGCATT	20	7.03354E-4	45.000004	43
TAGACTC	20	7.03354E-4	45.000004	34
TAAGTCG	40	6.8157533E-9	45.000004	1
ATAGACG	40	6.8157533E-9	45.000004	1
TCGATGC	20	7.03354E-4	45.000004	20
ATCGTAT	40	6.8157533E-9	45.000004	13
ATCCGAT	20	7.03354E-4	45.000004	32
ACTACGG	20	7.03354E-4	45.000004	2
TACTCTA	20	7.03354E-4	45.000004	28
GCGATCG	25	3.8910155E-5	45.000004	33
ACATGTC	25	3.8910155E-5	45.000004	19
ACGTAAG	20	7.03354E-4	45.000004	1
GTCCACA	20	7.03354E-4	45.000004	21
CCAGTAC	40	6.8157533E-9	45.000004	33
CGATCGC	25	3.8910155E-5	45.000004	34
GCATGCG	25	3.8910155E-5	45.000004	1
ATGCACG	65	0.0	45.000004	1
>>END_MODULE