FastQCFastQC Report
Thu 26 May 2016
SRR1545246_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545246_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3070071
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185950.6056863180037205No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA86930.28315306062954243No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT110350.041.289081
TAGGGCG12800.039.0234385
GACCGAT12000.038.43759
TAGTACG1000.038.251
AGGGCGA25800.038.1104626
ACGGGAT8700.038.017245
GGACGTA301.1403664E-437.5000048
GGACCGA13000.037.3846178
CGAGGGA15050.037.3754164
ACGGGTA5600.037.366075
AACGGGA7300.037.294524
GGGCGAT47250.037.2857137
CGACCCG9550.037.22513232
ATAGGGC17000.037.1911774
TATCGAG2300.037.1739161
ACGGGAC7150.037.1328665
CGAGGGT5400.037.0833364
TATTGCG1700.037.0588231
CGTTGAT7050.037.02127525
TACGGGT3350.036.94034