##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545246_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3070071
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.352340385613232	31.0	31.0	34.0	30.0	34.0
2	31.62615294564849	31.0	31.0	34.0	30.0	34.0
3	31.70529541499203	31.0	31.0	34.0	30.0	34.0
4	35.46498175449363	37.0	35.0	37.0	33.0	37.0
5	35.34149210229992	37.0	35.0	37.0	33.0	37.0
6	35.32850054607857	37.0	35.0	37.0	33.0	37.0
7	35.924309893810275	37.0	35.0	37.0	35.0	37.0
8	36.02317112535834	37.0	35.0	37.0	35.0	37.0
9	37.706148815450845	39.0	38.0	39.0	35.0	39.0
10	36.887028997049256	39.0	37.0	39.0	32.0	39.0
11	36.60532834582653	39.0	35.0	39.0	32.0	39.0
12	35.95645833597985	37.0	35.0	39.0	31.0	39.0
13	35.67265252171693	37.0	35.0	39.0	31.0	39.0
14	36.4906883261006	38.0	35.0	41.0	31.0	41.0
15	36.82850526909638	38.0	35.0	41.0	31.0	41.0
16	36.68050120013511	38.0	35.0	41.0	31.0	41.0
17	36.76605785338515	38.0	35.0	40.0	32.0	41.0
18	36.784870121896205	38.0	35.0	40.0	32.0	41.0
19	36.742012155419204	38.0	35.0	40.0	31.0	41.0
20	36.648597703440736	38.0	35.0	40.0	31.0	41.0
21	36.55981962632134	38.0	35.0	40.0	31.0	41.0
22	36.47433463265182	38.0	35.0	40.0	31.0	41.0
23	36.40260958134193	38.0	35.0	40.0	31.0	41.0
24	36.198689541707665	38.0	35.0	40.0	31.0	41.0
25	36.179801379186344	38.0	35.0	40.0	31.0	41.0
26	36.079650275189074	38.0	35.0	40.0	30.0	41.0
27	36.063501137270116	38.0	34.0	40.0	30.0	41.0
28	36.08152221886725	38.0	35.0	40.0	30.0	41.0
29	36.0583569565655	37.0	35.0	40.0	30.0	41.0
30	35.95816350827066	37.0	35.0	40.0	30.0	41.0
31	35.86206703362886	37.0	34.0	40.0	30.0	41.0
32	35.72858543010894	37.0	34.0	40.0	30.0	41.0
33	35.57082034910593	37.0	34.0	40.0	29.0	41.0
34	35.40958075562422	37.0	34.0	40.0	29.0	41.0
35	35.22965885805247	37.0	34.0	40.0	27.0	41.0
36	35.0769487090038	37.0	34.0	40.0	27.0	41.0
37	34.919849736374175	37.0	34.0	40.0	26.0	41.0
38	34.948129212646876	37.0	34.0	40.0	27.0	41.0
39	34.93301913864533	37.0	34.0	40.0	27.0	41.0
40	34.81968853489056	37.0	34.0	40.0	26.0	41.0
41	34.80119515151278	37.0	34.0	40.0	26.0	41.0
42	34.74705861851403	36.0	33.0	40.0	26.0	41.0
43	34.621289214483966	36.0	33.0	40.0	26.0	41.0
44	34.55692751079698	36.0	33.0	40.0	26.0	41.0
45	34.50033435708816	36.0	33.0	40.0	25.0	41.0
46	34.41515652243873	36.0	33.0	40.0	25.0	41.0
47	34.43694396644247	36.0	33.0	40.0	25.0	41.0
48	34.40573882493271	36.0	33.0	40.0	25.0	41.0
49	34.394126064185485	36.0	33.0	40.0	25.0	41.0
50	34.20334383146188	35.0	33.0	40.0	24.0	41.0
51	34.03585552255958	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	4.0
13	6.0
14	22.0
15	75.0
16	205.0
17	521.0
18	1249.0
19	2436.0
20	4524.0
21	7240.0
22	11299.0
23	16195.0
24	23673.0
25	33371.0
26	44189.0
27	52592.0
28	60371.0
29	69928.0
30	85334.0
31	105412.0
32	133985.0
33	176052.0
34	286506.0
35	403973.0
36	236965.0
37	291909.0
38	412952.0
39	608745.0
40	334.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.889685938859397	23.756681848725975	29.47208712762669	14.881545084787943
2	32.20638871218288	25.479019866315795	28.945454355941607	13.369137065559721
3	28.663213326336752	25.533350857358023	32.12251443044802	13.6809213858572
4	25.426284929566776	28.22319092946059	31.471389423892802	14.879134717079834
5	22.596024652198597	32.56953992269234	30.57798989013609	14.25644553497297
6	20.91557491667131	40.61453301894321	28.007072149145735	10.462819915239745
7	86.92649127658612	4.64393168757335	6.762319177634654	1.6672578582058852
8	89.0739660418277	3.0354672579233513	6.370308699701082	1.52025800054787
9	85.0355578095751	4.416477664523068	7.97108601071441	2.576878515187434
10	47.004417813138524	24.971148875710043	15.527751638317158	12.496681672834278
11	38.87809109300729	24.931801251502	22.383684286128886	13.806423369361816
12	35.026486358133084	23.208779210643662	26.996834926618963	14.76789950460429
13	21.747347211188277	35.86376341133479	26.923090703765485	15.465798673711456
14	16.587824841835904	37.46193491942043	29.964714171105488	15.985526067638176
15	16.054840425514588	24.59161367929276	43.99510630210181	15.358439593090845
16	18.566313287217138	21.276967210204585	41.58053673677254	18.576182765805743
17	18.83839168540402	21.665036411210036	30.413922023301744	29.082649880084205
18	21.195926739153588	24.112732246257497	35.7429844456366	18.948356568952317
19	28.107688714690966	24.741251912415056	28.03762518847284	19.11343418442114
20	28.69529727488387	25.252966462339145	27.959060230203146	18.09267603257384
21	22.190724579333835	29.01584360752569	30.233958758608516	18.559473054531963
22	21.574517332009584	25.50445901739732	27.522523094742763	25.39850055585034
23	19.238186999584048	29.846052420286046	28.41644378908501	22.499316791044897
24	18.819141316275747	24.58920331158465	39.304791322415674	17.28686404972393
25	18.772302008650616	26.77687258698577	35.515660712732696	18.93516469163091
26	17.92623037056798	32.91005322026754	28.670965590046617	20.492750819117862
27	17.2094391302351	33.90755458098526	30.152755424874538	18.730250863905102
28	16.461541117452985	28.982815055417284	36.639478370369936	17.916165456759796
29	17.301163393289603	26.306655448685063	35.89086376178271	20.501317396242627
30	19.218610905089818	31.656010561319263	31.315692698963637	17.809685834627278
31	27.145072540667627	27.825154532256747	28.049839889696358	16.979933037379265
32	28.220780561752477	26.023404670445732	28.53412836380657	17.221686403995218
33	27.269239050171805	26.745537806780362	26.966900765487186	19.018322377560647
34	20.320832970963863	27.088135746697716	29.37629129749768	23.21473998484074
35	20.389430733035162	26.644888668698542	32.44859809431118	20.51708250395512
36	27.70473386446112	27.74066137232657	26.59156742629079	17.963037336921523
37	20.52691941000713	33.72365003936391	28.505399386528847	17.24403116410011
38	20.526561112104574	33.313268650790164	26.361247020020055	19.798923217085207
39	20.72173575138816	32.447099757627754	27.52095961298615	19.310204877997936
40	24.2664420464543	26.32427719098353	27.623074515214796	21.786206247347373
41	17.80561426755277	26.446000760243006	29.249714420285393	26.498670551918828
42	19.685245064364963	26.418444394282737	27.3320714732656	26.5642390680867
43	19.89312299292101	26.110568778376788	29.287824288102783	24.708483940599418
44	18.54966872101655	29.2603982122889	31.204392341414906	20.985540725279645
45	17.838317094295213	35.723538641288755	26.713942446282186	19.72420181813385
46	20.530437243959504	33.144184613320014	27.48275202755897	18.842626115161508
47	19.50687785396494	28.915878492712384	28.365728349604947	23.211515303717732
48	19.86719525379055	27.136766543835634	32.778101874516906	20.217936327856915
49	20.767663027988604	26.85533982764568	33.181480167722505	19.195516976643212
50	19.88716221872393	33.868858407509144	27.6420317315137	18.601947642253226
51	18.163293291914094	33.182196763527614	27.880364981787064	20.774144962771217
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	9703.0
1	8354.0
2	7005.0
3	19664.5
4	32324.0
5	22729.5
6	13135.0
7	12978.5
8	12822.0
9	13514.0
10	14206.0
11	14529.5
12	14853.0
13	14920.5
14	14988.0
15	14075.0
16	13162.0
17	13065.5
18	12969.0
19	13608.0
20	14247.0
21	14859.5
22	15472.0
23	18127.5
24	20783.0
25	23757.0
26	31222.5
27	35714.0
28	43001.0
29	50288.0
30	59213.5
31	68139.0
32	77922.5
33	87706.0
34	101373.5
35	115041.0
36	124793.5
37	134546.0
38	148354.5
39	162163.0
40	192743.5
41	223324.0
42	256620.0
43	289916.0
44	284792.5
45	279669.0
46	268242.5
47	256816.0
48	240669.0
49	224522.0
50	206876.5
51	189231.0
52	169273.5
53	149316.0
54	134212.0
55	119108.0
56	112011.0
57	104914.0
58	97328.5
59	89743.0
60	82976.0
61	76209.0
62	67480.5
63	58752.0
64	51429.5
65	44107.0
66	36092.0
67	28077.0
68	24252.5
69	20428.0
70	17521.0
71	14614.0
72	12812.0
73	11010.0
74	9453.0
75	5419.5
76	2943.0
77	2390.5
78	1838.0
79	1335.0
80	832.0
81	685.0
82	538.0
83	328.5
84	119.0
85	90.0
86	61.0
87	51.0
88	41.0
89	39.5
90	38.0
91	24.0
92	10.0
93	6.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3070071.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.341806358338168
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.49161134910871	12.663529070627346
2	7.0922491633553335	2.318003249452775
3	2.69752889429801	1.3224748450992045
4	1.5117291419868248	0.988175396184216
5	0.9928103146083629	0.811215695594533
6	0.7293939849066715	0.7151769156168876
7	0.5406109086162875	0.6184191148668835
8	0.4546796942383176	0.5944230014648795
9	0.3643866037154312	0.5359261785741075
>10	5.008221047941632	19.952875993483612
>50	1.7700889873999637	20.248976255755686
>100	1.3382331406683787	37.26706555619146
>500	0.007219193182106897	0.7367685867711207
>1k	8.250506493836454E-4	0.30717229990141925
>5k	2.0626266234591135E-4	0.29301534105596816
>10k+	2.0626266234591135E-4	0.6267824993599135
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18595	0.6056863180037205	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8693	0.28315306062954243	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0077196911732660255	0.0
2	0.0	0.0	0.0	0.041073968647630625	0.0
3	0.0	0.0	0.0	0.07211559602367502	0.0
4	0.0	0.0	0.0	0.10944372296275884	0.0
5	0.0	0.0	0.0	0.20699847006795608	0.0
6	3.257253659605918E-5	0.0	0.0	0.3421419244050056	0.0
7	3.257253659605918E-5	0.0	0.0	0.42858943653094667	0.0
8	3.257253659605918E-5	0.0	0.0	0.6072823722969274	0.0
9	3.257253659605918E-5	0.0	0.0	0.7067914715978881	0.0
10	3.257253659605918E-5	0.0	0.0	0.856950865305721	0.0
11	3.257253659605918E-5	0.0	0.0	1.0023546686705291	0.0
12	3.257253659605918E-5	0.0	0.0	1.1198763807091106	0.0
13	3.257253659605918E-5	0.0	0.0	1.1715364237504604	0.0
14	3.257253659605918E-5	0.0	0.0	1.1954120930753718	0.0
15	6.514507319211836E-5	0.0	0.0	1.2227730238160617	0.0
16	6.514507319211836E-5	0.0	0.0	1.2851168588609188	0.0
17	1.302901463842367E-4	0.0	0.0	1.3532586054198747	0.0
18	1.302901463842367E-4	0.0	0.0	1.4537448808187172	0.0
19	1.302901463842367E-4	0.0	0.0	1.5063495274213528	0.0
20	1.302901463842367E-4	0.0	0.0	1.5657618341725648	0.0
21	1.302901463842367E-4	0.0	0.0	1.6387894612209295	0.0
22	2.2800775617241425E-4	0.0	0.0	1.7207093907600182	0.0
23	2.2800775617241425E-4	0.0	0.0	1.813573692595383	0.0
24	3.257253659605918E-4	0.0	0.0	1.8868944724731123	0.0
25	3.257253659605918E-4	0.0	0.0	1.949987475859679	0.0
26	3.257253659605918E-4	0.0	0.0	2.0123964559777283	0.0
27	3.257253659605918E-4	0.0	0.0	2.0787141404873046	0.0
28	3.257253659605918E-4	0.0	0.0	2.147702772997758	0.0
29	3.257253659605918E-4	0.0	0.0	2.2288409616585416	0.0
30	3.257253659605918E-4	0.0	0.0	2.337600661352783	0.0
31	3.9087043915271016E-4	0.0	0.0	2.4253510749425664	0.0
32	4.2344297574876933E-4	0.0	0.0	2.51310148853235	0.0
33	4.2344297574876933E-4	0.0	0.0	2.598441534414025	0.0
34	4.2344297574876933E-4	0.0	0.0	2.6927390278596164	0.0
35	4.2344297574876933E-4	0.0	0.0	2.808729830678183	0.0
36	4.2344297574876933E-4	0.0	0.0	2.9075874792472227	0.0
37	4.2344297574876933E-4	0.0	0.0	3.01390423869676	0.0
38	4.2344297574876933E-4	0.0	0.0	3.1305790647838436	0.0
39	4.2344297574876933E-4	0.0	0.0	3.292073701227105	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	11035	0.0	41.28908	1
TAGGGCG	1280	0.0	39.023438	5
GACCGAT	1200	0.0	38.4375	9
TAGTACG	100	0.0	38.25	1
AGGGCGA	2580	0.0	38.110462	6
ACGGGAT	870	0.0	38.01724	5
GGACGTA	30	1.1403664E-4	37.500004	8
GGACCGA	1300	0.0	37.384617	8
CGAGGGA	1505	0.0	37.375416	4
ACGGGTA	560	0.0	37.36607	5
AACGGGA	730	0.0	37.29452	4
GGGCGAT	4725	0.0	37.285713	7
CGACCCG	955	0.0	37.225132	32
ATAGGGC	1700	0.0	37.191177	4
TATCGAG	230	0.0	37.173916	1
ACGGGAC	715	0.0	37.132866	5
CGAGGGT	540	0.0	37.083336	4
TATTGCG	170	0.0	37.058823	1
CGTTGAT	705	0.0	37.021275	25
TACGGGT	335	0.0	36.9403	4
>>END_MODULE