FastQCFastQC Report
Thu 26 May 2016
SRR1545245_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545245_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences168748
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7410.4391163154526275No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4430.2625216298859838No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT2960.17540948633465284No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC2270.1345201128309669No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG1790.10607533126318533No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCGG207.016165E-445.027
TGGGTAT406.7557266E-945.06
AGGGTAC800.045.06
TGCTGTA207.016165E-445.026
TGATTCA406.7557266E-945.044
TAGGGCG850.045.05
GCGATGT351.203698E-745.09
CCCCATT800.045.042
TATAGCG207.016165E-445.01
CGGTAGT207.016165E-445.012
CCTATGC406.7557266E-945.035
CGTCCCC800.045.039
CGATCAA207.016165E-445.010
CTAGGGA453.8016879E-1045.04
AATACGG207.016165E-445.02
GCTTTGA406.7557266E-945.040
AGCACGG207.016165E-445.02
GCCTTAT302.1544656E-644.99999625
CATGCAT600.044.99999625
CCAGGGA253.8766215E-544.9999964